Membrane biofilm communities in full-scale membrane bioreactors are not randomly assembled and consist of a core microbiome

Handle URI:
http://hdl.handle.net/10754/625148
Title:
Membrane biofilm communities in full-scale membrane bioreactors are not randomly assembled and consist of a core microbiome
Authors:
Matar, Gerald Kamil ( 0000-0002-2106-4826 ) ; Bagchi, Samik; Zhang, Kai; Oerther, Daniel B.; Saikaly, Pascal ( 0000-0001-7678-3986 )
Abstract:
Finding efficient biofouling control strategies requires a better understanding of the microbial ecology of membrane biofilm communities in membrane bioreactors (MBRs). Studies that characterized the membrane biofilm communities in lab-and pilot-scale MBRs are numerous, yet similar studies in full-scale MBRs are limited. Also, most of these studies have characterized the mature biofilm communities with very few studies addressing early biofilm communities. In this study, five full-scale MBRs located in Seattle (Washington, U.S.A.) were selected to address two questions concerning membrane biofilm communities (early and mature): (i) Is the assembly of biofilm communities (early and mature) the result of random immigration of species from the source community (i.e. activated sludge)? and (ii) Is there a core membrane biofilm community in full-scale MBRs? Membrane biofilm (early and mature) and activated sludge (AS) samples were collected from the five MBRs, and 16S rRNA gene sequencing was applied to investigate the bacterial communities of AS and membrane biofilms (early and mature). Alpha and beta diversity measures revealed clear differences in the bacterial community structure between the AS and biofilm (early and mature) samples in the five full-scale MBRs. These differences were mainly due to the presence of large number of unique but rare operational taxonomic units (∼13% of total reads in each MBR) in each sample. In contrast, a high percentage (∼87% of total reads in each MBR) of sequence reads was shared between AS and biofilm samples in each MBR, and these shared sequence reads mainly belong to the dominant taxa in these samples. Despite the large fraction of shared sequence reads between AS and biofilm samples, simulated biofilm communities from random sampling of the respective AS community revealed that biofilm communities differed significantly from the random assemblages (P < 0.001 for each MBR), indicating that the biofilm communities (early and mature) are unlikely to represent a random sample of the AS community. In addition to the presence of unique operational taxonomic units in each biofilm sample (early or mature), comparative analysis of operational taxonomic units and genera revealed the presence of a core biofilm community in the five full-scale MBRs. These findings provided insight into the membrane biofilm communities in full-scale MBRs. More comparative studies are needed in the future to elucidate the factors shaping the core and unique biofilm communities in full-scale MBRs.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Water Desalination and Reuse Research Center (WDRC)
Citation:
Matar GK, Bagchi S, Zhang K, Oerther DB, Saikaly PE (2017) Membrane biofilm communities in full-scale membrane bioreactors are not randomly assembled and consist of a core microbiome. Water Research 123: 124–133. Available: http://dx.doi.org/10.1016/j.watres.2017.06.052.
Publisher:
Elsevier BV
Journal:
Water Research
Issue Date:
21-Jun-2017
DOI:
10.1016/j.watres.2017.06.052
Type:
Article
ISSN:
0043-1354
Sponsors:
This work was sponsored by King Abdullah University of Science and Technology (KAUST). We thank Prof. Regina Lamendella for NMDS analysis (Fig. 4).
Additional Links:
http://www.sciencedirect.com/science/article/pii/S0043135417305298
Appears in Collections:
Articles; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorMatar, Gerald Kamilen
dc.contributor.authorBagchi, Samiken
dc.contributor.authorZhang, Kaien
dc.contributor.authorOerther, Daniel B.en
dc.contributor.authorSaikaly, Pascalen
dc.date.accessioned2017-07-03T11:45:32Z-
dc.date.available2017-07-03T11:45:32Z-
dc.date.issued2017-06-21en
dc.identifier.citationMatar GK, Bagchi S, Zhang K, Oerther DB, Saikaly PE (2017) Membrane biofilm communities in full-scale membrane bioreactors are not randomly assembled and consist of a core microbiome. Water Research 123: 124–133. Available: http://dx.doi.org/10.1016/j.watres.2017.06.052.en
dc.identifier.issn0043-1354en
dc.identifier.doi10.1016/j.watres.2017.06.052en
dc.identifier.urihttp://hdl.handle.net/10754/625148-
dc.description.abstractFinding efficient biofouling control strategies requires a better understanding of the microbial ecology of membrane biofilm communities in membrane bioreactors (MBRs). Studies that characterized the membrane biofilm communities in lab-and pilot-scale MBRs are numerous, yet similar studies in full-scale MBRs are limited. Also, most of these studies have characterized the mature biofilm communities with very few studies addressing early biofilm communities. In this study, five full-scale MBRs located in Seattle (Washington, U.S.A.) were selected to address two questions concerning membrane biofilm communities (early and mature): (i) Is the assembly of biofilm communities (early and mature) the result of random immigration of species from the source community (i.e. activated sludge)? and (ii) Is there a core membrane biofilm community in full-scale MBRs? Membrane biofilm (early and mature) and activated sludge (AS) samples were collected from the five MBRs, and 16S rRNA gene sequencing was applied to investigate the bacterial communities of AS and membrane biofilms (early and mature). Alpha and beta diversity measures revealed clear differences in the bacterial community structure between the AS and biofilm (early and mature) samples in the five full-scale MBRs. These differences were mainly due to the presence of large number of unique but rare operational taxonomic units (∼13% of total reads in each MBR) in each sample. In contrast, a high percentage (∼87% of total reads in each MBR) of sequence reads was shared between AS and biofilm samples in each MBR, and these shared sequence reads mainly belong to the dominant taxa in these samples. Despite the large fraction of shared sequence reads between AS and biofilm samples, simulated biofilm communities from random sampling of the respective AS community revealed that biofilm communities differed significantly from the random assemblages (P < 0.001 for each MBR), indicating that the biofilm communities (early and mature) are unlikely to represent a random sample of the AS community. In addition to the presence of unique operational taxonomic units in each biofilm sample (early or mature), comparative analysis of operational taxonomic units and genera revealed the presence of a core biofilm community in the five full-scale MBRs. These findings provided insight into the membrane biofilm communities in full-scale MBRs. More comparative studies are needed in the future to elucidate the factors shaping the core and unique biofilm communities in full-scale MBRs.en
dc.description.sponsorshipThis work was sponsored by King Abdullah University of Science and Technology (KAUST). We thank Prof. Regina Lamendella for NMDS analysis (Fig. 4).en
dc.publisherElsevier BVen
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0043135417305298en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Water Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Water Research, 20 June 2017. DOI: 10.1016/j.watres.2017.06.052. © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectBiofoulingen
dc.subjectMembrane bioreactoren
dc.subjectActivated sludgeen
dc.subjectEarly biofilmen
dc.subjectMature biofilmen
dc.subject16S rRNA gene sequencingen
dc.titleMembrane biofilm communities in full-scale membrane bioreactors are not randomly assembled and consist of a core microbiomeen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.identifier.journalWater Researchen
dc.eprint.versionPost-printen
dc.contributor.institutionBaswood Corporation, Allen, TX 75013, USAen
dc.contributor.institutionDepartment of Civil, Architectural, and Environmental Engineering, And Environmental Research Center, Missouri University of Science and Technology, Rolla, MO 65409, USAen
kaust.authorMatar, Gerald Kamilen
kaust.authorBagchi, Samiken
kaust.authorSaikaly, Pascalen
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