Engineering Strategies to Decode and Enhance the Genomes of Coral Symbionts

Handle URI:
http://hdl.handle.net/10754/625146
Title:
Engineering Strategies to Decode and Enhance the Genomes of Coral Symbionts
Authors:
Levin, Rachel A.; Voolstra, Christian R. ( 0000-0003-4555-3795 ) ; Agrawal, Shobhit; Steinberg, Peter D.; Suggett, David J.; van Oppen, Madeleine J. H.
Abstract:
Elevated sea surface temperatures from a severe and prolonged El Niño event (2014–2016) fueled by climate change have resulted in mass coral bleaching (loss of dinoflagellate photosymbionts, Symbiodinium spp., from coral tissues) and subsequent coral mortality, devastating reefs worldwide. Genetic variation within and between Symbiodinium species strongly influences the bleaching tolerance of corals, thus recent papers have called for genetic engineering of Symbiodinium to elucidate the genetic basis of bleaching-relevant Symbiodinium traits. However, while Symbiodinium has been intensively studied for over 50 years, genetic transformation of Symbiodinium has seen little success likely due to the large evolutionary divergence between Symbiodinium and other model eukaryotes rendering standard transformation systems incompatible. Here, we integrate the growing wealth of Symbiodinium next-generation sequencing data to design tailored genetic engineering strategies. Specifically, we develop a testable expression construct model that incorporates endogenous Symbiodinium promoters, terminators, and genes of interest, as well as an internal ribosomal entry site from a Symbiodinium virus. Furthermore, we assess the potential for CRISPR/Cas9 genome editing through new analyses of the three currently available Symbiodinium genomes. Finally, we discuss how genetic engineering could be applied to enhance the stress tolerance of Symbiodinium, and in turn, coral reefs.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Red Sea Research Center (RSRC)
Citation:
Levin RA, Voolstra CR, Agrawal S, Steinberg PD, Suggett DJ, et al. (2017) Engineering Strategies to Decode and Enhance the Genomes of Coral Symbionts. Frontiers in Microbiology 8. Available: http://dx.doi.org/10.3389/fmicb.2017.01220.
Publisher:
Frontiers Media SA
Journal:
Frontiers in Microbiology
Issue Date:
30-Jun-2017
DOI:
10.3389/fmicb.2017.01220
Type:
Article
ISSN:
1664-302X
Sponsors:
Funding from the University of New South Wales and King Abdullah University of Science and Technology (KAUST) supported the analyses presented here.
Additional Links:
http://journal.frontiersin.org/article/10.3389/fmicb.2017.01220/full
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLevin, Rachel A.en
dc.contributor.authorVoolstra, Christian R.en
dc.contributor.authorAgrawal, Shobhiten
dc.contributor.authorSteinberg, Peter D.en
dc.contributor.authorSuggett, David J.en
dc.contributor.authorvan Oppen, Madeleine J. H.en
dc.date.accessioned2017-07-03T11:45:32Z-
dc.date.available2017-07-03T11:45:32Z-
dc.date.issued2017-06-30en
dc.identifier.citationLevin RA, Voolstra CR, Agrawal S, Steinberg PD, Suggett DJ, et al. (2017) Engineering Strategies to Decode and Enhance the Genomes of Coral Symbionts. Frontiers in Microbiology 8. Available: http://dx.doi.org/10.3389/fmicb.2017.01220.en
dc.identifier.issn1664-302Xen
dc.identifier.doi10.3389/fmicb.2017.01220en
dc.identifier.urihttp://hdl.handle.net/10754/625146-
dc.description.abstractElevated sea surface temperatures from a severe and prolonged El Niño event (2014–2016) fueled by climate change have resulted in mass coral bleaching (loss of dinoflagellate photosymbionts, Symbiodinium spp., from coral tissues) and subsequent coral mortality, devastating reefs worldwide. Genetic variation within and between Symbiodinium species strongly influences the bleaching tolerance of corals, thus recent papers have called for genetic engineering of Symbiodinium to elucidate the genetic basis of bleaching-relevant Symbiodinium traits. However, while Symbiodinium has been intensively studied for over 50 years, genetic transformation of Symbiodinium has seen little success likely due to the large evolutionary divergence between Symbiodinium and other model eukaryotes rendering standard transformation systems incompatible. Here, we integrate the growing wealth of Symbiodinium next-generation sequencing data to design tailored genetic engineering strategies. Specifically, we develop a testable expression construct model that incorporates endogenous Symbiodinium promoters, terminators, and genes of interest, as well as an internal ribosomal entry site from a Symbiodinium virus. Furthermore, we assess the potential for CRISPR/Cas9 genome editing through new analyses of the three currently available Symbiodinium genomes. Finally, we discuss how genetic engineering could be applied to enhance the stress tolerance of Symbiodinium, and in turn, coral reefs.en
dc.description.sponsorshipFunding from the University of New South Wales and King Abdullah University of Science and Technology (KAUST) supported the analyses presented here.en
dc.publisherFrontiers Media SAen
dc.relation.urlhttp://journal.frontiersin.org/article/10.3389/fmicb.2017.01220/fullen
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleEngineering Strategies to Decode and Enhance the Genomes of Coral Symbiontsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalFrontiers in Microbiologyen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionCentre for Marine Bio-Innovation, The University of New South Wales, Sydney, NSW, Australiaen
dc.contributor.institutionSchool of Biological, Earth and Environmental Sciences, The University of New South Wales, Sydney, NSW, Australiaen
dc.contributor.institutionClimate Change Cluster, University of Technology Sydney, Ultimo, NSW, Australiaen
dc.contributor.institutionSydney Institute of Marine Science, Mosman, NSW, Australiaen
dc.contributor.institutionAustralian Institute of Marine Science, Townsville, QLD, Australiaen
dc.contributor.institutionSchool of BioSciences, The University of Melbourne, Parkville, VIC, Australiaen
kaust.authorVoolstra, Christian R.en
kaust.authorAgrawal, Shobhiten
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