Comparative proteome analysis between C . briggsae embryos and larvae reveals a role of chromatin modification proteins in embryonic cell division

Handle URI:
http://hdl.handle.net/10754/625145
Title:
Comparative proteome analysis between C . briggsae embryos and larvae reveals a role of chromatin modification proteins in embryonic cell division
Authors:
An, Xiaomeng; Shao, Jiaofang; Zhang, Huoming ( 0000-0001-5416-0358 ) ; Ren, Xiaoliang; Ho, Vincy Wing Sze; Li, Runsheng; Wong, Ming-Kin; Zhao, Zhongying ( 0000-0003-2743-9008 )
Abstract:
Caenorhabditis briggsae has emerged as a model for comparative biology against model organism C. elegans. Most of its cell fate specifications are completed during embryogenesis whereas its cell growth is achieved mainly in larval stages. The molecular mechanism underlying the drastic developmental changes is poorly understood. To gain insights into the molecular changes between the two stages, we compared the proteomes between the two stages using iTRAQ. We identified a total of 2,791 proteins in the C. briggsae embryos and larvae, 247 of which undergo up- or down-regulation between the two stages. The proteins that are upregulated in the larval stages are enriched in the Gene Ontology categories of energy production, protein translation, and cytoskeleton; whereas those upregulated in the embryonic stage are enriched in the categories of chromatin dynamics and posttranslational modification, suggesting a more active chromatin modification in the embryos than in the larva. Perturbation of a subset of chromatin modifiers followed by cell lineage analysis suggests their roles in controlling cell division pace. Taken together, we demonstrate a general molecular switch from chromatin modification to metabolism during the transition from C. briggsae embryonic to its larval stages using iTRAQ approach. The switch might be conserved across metazoans.
KAUST Department:
Biosciences Core Lab
Citation:
An X, Shao J, Zhang H, Ren X, Ho VWS, et al. (2017) Comparative proteome analysis between C . briggsae embryos and larvae reveals a role of chromatin modification proteins in embryonic cell division. Scientific Reports 7. Available: http://dx.doi.org/10.1038/s41598-017-04533-8.
Publisher:
Springer Nature
Journal:
Scientific Reports
Issue Date:
21-Jun-2017
DOI:
10.1038/s41598-017-04533-8
Type:
Article
ISSN:
2045-2322
Sponsors:
We thank Mr. Chung Wai Shing for the logistic support and the members of Zhao’s lab for helpful discussion and comments. This work was supported by General Research Fund (HKBU12103314, HKBU263512, HKBU12123716) and Collaborative Research Fund (HKBU5/CRF/11G) both from Hong Kong Research Grant Council to ZZ.
Additional Links:
https://www.nature.com/articles/s41598-017-04533-8
Appears in Collections:
Articles; Bioscience Core Lab

Full metadata record

DC FieldValue Language
dc.contributor.authorAn, Xiaomengen
dc.contributor.authorShao, Jiaofangen
dc.contributor.authorZhang, Huomingen
dc.contributor.authorRen, Xiaoliangen
dc.contributor.authorHo, Vincy Wing Szeen
dc.contributor.authorLi, Runshengen
dc.contributor.authorWong, Ming-Kinen
dc.contributor.authorZhao, Zhongyingen
dc.date.accessioned2017-07-03T11:45:31Z-
dc.date.available2017-07-03T11:45:31Z-
dc.date.issued2017-06-21en
dc.identifier.citationAn X, Shao J, Zhang H, Ren X, Ho VWS, et al. (2017) Comparative proteome analysis between C . briggsae embryos and larvae reveals a role of chromatin modification proteins in embryonic cell division. Scientific Reports 7. Available: http://dx.doi.org/10.1038/s41598-017-04533-8.en
dc.identifier.issn2045-2322en
dc.identifier.doi10.1038/s41598-017-04533-8en
dc.identifier.urihttp://hdl.handle.net/10754/625145-
dc.description.abstractCaenorhabditis briggsae has emerged as a model for comparative biology against model organism C. elegans. Most of its cell fate specifications are completed during embryogenesis whereas its cell growth is achieved mainly in larval stages. The molecular mechanism underlying the drastic developmental changes is poorly understood. To gain insights into the molecular changes between the two stages, we compared the proteomes between the two stages using iTRAQ. We identified a total of 2,791 proteins in the C. briggsae embryos and larvae, 247 of which undergo up- or down-regulation between the two stages. The proteins that are upregulated in the larval stages are enriched in the Gene Ontology categories of energy production, protein translation, and cytoskeleton; whereas those upregulated in the embryonic stage are enriched in the categories of chromatin dynamics and posttranslational modification, suggesting a more active chromatin modification in the embryos than in the larva. Perturbation of a subset of chromatin modifiers followed by cell lineage analysis suggests their roles in controlling cell division pace. Taken together, we demonstrate a general molecular switch from chromatin modification to metabolism during the transition from C. briggsae embryonic to its larval stages using iTRAQ approach. The switch might be conserved across metazoans.en
dc.description.sponsorshipWe thank Mr. Chung Wai Shing for the logistic support and the members of Zhao’s lab for helpful discussion and comments. This work was supported by General Research Fund (HKBU12103314, HKBU263512, HKBU12123716) and Collaborative Research Fund (HKBU5/CRF/11G) both from Hong Kong Research Grant Council to ZZ.en
dc.publisherSpringer Natureen
dc.relation.urlhttps://www.nature.com/articles/s41598-017-04533-8en
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleComparative proteome analysis between C . briggsae embryos and larvae reveals a role of chromatin modification proteins in embryonic cell divisionen
dc.typeArticleen
dc.contributor.departmentBiosciences Core Laben
dc.identifier.journalScientific Reportsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionCore Laboratory, The University of Hong Kong-Shenzhen Hospital, Shenzhen, China.en
dc.contributor.institutionDepartment of Bioinformatics, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, China.en
dc.contributor.institutionDepartment of Biology, Hong Kong Baptist University, Hong Kong, China.en
dc.contributor.institutionDepartment of Biology, Hong Kong Baptist University, Hong Kong, China. zyzhao@hkbu.edu.hk.en
kaust.authorZhang, Huomingen
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