Handle URI:
http://hdl.handle.net/10754/625119
Title:
Plasmonics Enhanced Smartphone Fluorescence Microscopy
Authors:
Wei, Qingshan; Acuna, Guillermo; Kim, Seungkyeum; Vietz, Carolin; Tseng, Derek; Chae, Jongjae ( 0000-0002-6560-197X ) ; Shir, Daniel; Luo, Wei; Tinnefeld, Philip; Ozcan, Aydogan ( 0000-0002-0717-683X )
Abstract:
Smartphone fluorescence microscopy has various applications in point-of-care (POC) testing and diagnostics, ranging from e.g., quantification of immunoassays, detection of microorganisms, to sensing of viruses. An important need in smartphone-based microscopy and sensing techniques is to improve the detection sensitivity to enable quantification of extremely low concentrations of target molecules. Here, we demonstrate a general strategy to enhance the detection sensitivity of a smartphone-based fluorescence microscope by using surface-enhanced fluorescence (SEF) created by a thin metal-film. In this plasmonic design, the samples are placed on a silver-coated glass slide with a thin spacer, and excited by a laser-diode from the backside through a glass hemisphere, generating surface plasmon polaritons. We optimized this mobile SEF system by tuning the metal-film thickness, spacer distance, excitation angle and polarization, and achieved ~10-fold enhancement in fluorescence intensity compared to a bare glass substrate, which enabled us to image single fluorescent particles as small as 50 nm in diameter and single quantum-dots. Furthermore, we quantified the detection limit of this platform by using DNA origami-based brightness standards, demonstrating that ~80 fluorophores per diffraction-limited spot can be readily detected by our mobile microscope, which opens up new opportunities for POC diagnostics and sensing applications in resource-limited-settings.
Citation:
Wei Q, Acuna G, Kim S, Vietz C, Tseng D, et al. (2017) Plasmonics Enhanced Smartphone Fluorescence Microscopy. Scientific Reports 7. Available: http://dx.doi.org/10.1038/s41598-017-02395-8.
Publisher:
Springer Nature
Journal:
Scientific Reports
Issue Date:
12-May-2017
DOI:
10.1038/s41598-017-02395-8
Type:
Article
ISSN:
2045-2322
Sponsors:
The Ozcan Research Group at UCLA gratefully acknowledges the support of the Presidential Early Career Award for Scientists and Engineers (PECASE), the Army Research Office (ARO; W911NF-13-1-0419 and W911NF-13-1-0197), the ARO Life Sciences Division, the National Science Foundation (NSF) CBET Division Biophotonics Program, the NSF Emerging Frontiers in Research and Innovation (EFRI) Award, the NSF EAGER Award, NSF INSPIRE Award, NSF Partnerships for Innovation: Building Innovation Capacity (PFI:BIC) Program, Office of Naval Research (ONR), the National Institutes of Health (NIH), the Howard Hughes Medical Institute (HHMI), Vodafone Americas Foundation, the Mary Kay Foundation, Steven & Alexandra Cohen Foundation, and KAUST. This work is based upon research performed in a laboratory renovated by the National Science Foundation under Grant No. 0963183, which is an award funded under the American Recovery and Reinvestment Act of 2009 (ARRA). G.A. acknowlegdes support from Deutsche Forschungsgemeinschaft (DFG, AC 279/3-1). C.V. is greatful for funding of the Studienstiftung des Deutschen Volkes.
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorWei, Qingshanen
dc.contributor.authorAcuna, Guillermoen
dc.contributor.authorKim, Seungkyeumen
dc.contributor.authorVietz, Carolinen
dc.contributor.authorTseng, Dereken
dc.contributor.authorChae, Jongjaeen
dc.contributor.authorShir, Danielen
dc.contributor.authorLuo, Weien
dc.contributor.authorTinnefeld, Philipen
dc.contributor.authorOzcan, Aydoganen
dc.date.accessioned2017-06-21T06:51:52Z-
dc.date.available2017-06-21T06:51:52Z-
dc.date.issued2017-05-12en
dc.identifier.citationWei Q, Acuna G, Kim S, Vietz C, Tseng D, et al. (2017) Plasmonics Enhanced Smartphone Fluorescence Microscopy. Scientific Reports 7. Available: http://dx.doi.org/10.1038/s41598-017-02395-8.en
dc.identifier.issn2045-2322en
dc.identifier.doi10.1038/s41598-017-02395-8en
dc.identifier.urihttp://hdl.handle.net/10754/625119-
dc.description.abstractSmartphone fluorescence microscopy has various applications in point-of-care (POC) testing and diagnostics, ranging from e.g., quantification of immunoassays, detection of microorganisms, to sensing of viruses. An important need in smartphone-based microscopy and sensing techniques is to improve the detection sensitivity to enable quantification of extremely low concentrations of target molecules. Here, we demonstrate a general strategy to enhance the detection sensitivity of a smartphone-based fluorescence microscope by using surface-enhanced fluorescence (SEF) created by a thin metal-film. In this plasmonic design, the samples are placed on a silver-coated glass slide with a thin spacer, and excited by a laser-diode from the backside through a glass hemisphere, generating surface plasmon polaritons. We optimized this mobile SEF system by tuning the metal-film thickness, spacer distance, excitation angle and polarization, and achieved ~10-fold enhancement in fluorescence intensity compared to a bare glass substrate, which enabled us to image single fluorescent particles as small as 50 nm in diameter and single quantum-dots. Furthermore, we quantified the detection limit of this platform by using DNA origami-based brightness standards, demonstrating that ~80 fluorophores per diffraction-limited spot can be readily detected by our mobile microscope, which opens up new opportunities for POC diagnostics and sensing applications in resource-limited-settings.en
dc.description.sponsorshipThe Ozcan Research Group at UCLA gratefully acknowledges the support of the Presidential Early Career Award for Scientists and Engineers (PECASE), the Army Research Office (ARO; W911NF-13-1-0419 and W911NF-13-1-0197), the ARO Life Sciences Division, the National Science Foundation (NSF) CBET Division Biophotonics Program, the NSF Emerging Frontiers in Research and Innovation (EFRI) Award, the NSF EAGER Award, NSF INSPIRE Award, NSF Partnerships for Innovation: Building Innovation Capacity (PFI:BIC) Program, Office of Naval Research (ONR), the National Institutes of Health (NIH), the Howard Hughes Medical Institute (HHMI), Vodafone Americas Foundation, the Mary Kay Foundation, Steven & Alexandra Cohen Foundation, and KAUST. This work is based upon research performed in a laboratory renovated by the National Science Foundation under Grant No. 0963183, which is an award funded under the American Recovery and Reinvestment Act of 2009 (ARRA). G.A. acknowlegdes support from Deutsche Forschungsgemeinschaft (DFG, AC 279/3-1). C.V. is greatful for funding of the Studienstiftung des Deutschen Volkes.en
dc.publisherSpringer Natureen
dc.titlePlasmonics Enhanced Smartphone Fluorescence Microscopyen
dc.typeArticleen
dc.identifier.journalScientific Reportsen
dc.contributor.institutionElectrical Engineering Department, University of California, Los Angeles, Los Angeles, CA, 90095, USAen
dc.contributor.institutionBioengineering Department, University of California, Los Angeles, Los Angeles, CA, 90095, USAen
dc.contributor.institutionCalifornia NanoSystems Institute (CNSI), University of California, Los Angeles, Los Angeles, CA, 90095, USAen
dc.contributor.institutionDepartment of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC, 27695, USAen
dc.contributor.institutionInstitute for Physical & Theoretical Chemistry, Braunschweig University of Technology, Braunschweig, 38106, Germanyen
dc.contributor.institutionBraunschweig Integrated Centre of Systems Biology (BRICS), Braunschweig University of Technology, Braunschweig, 38106, Germanyen
dc.contributor.institutionLaboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology, Braunschweig, 38106, Germanyen
dc.contributor.institutionChemical and Biomolecular Engineering Department, University of California, Los Angeles, Los Angeles, CA, 90095, USAen
dc.contributor.institutionDepartment of Surgery, University of California, Los Angeles, Los Angeles, CA, 90095, USAen
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