Laser-Scribed Graphene Electrodes for Aptamer-Based Biosensing

Handle URI:
http://hdl.handle.net/10754/625082
Title:
Laser-Scribed Graphene Electrodes for Aptamer-Based Biosensing
Authors:
Fenzl, Christoph; Nayak, Pranati; Hirsch, Thomas; Wolfbeis, Otto S.; Alshareef, Husam N. ( 0000-0001-5029-2142 ) ; Baeumner, Antje J.
Abstract:
Graphene as a transducer material has produced some of the best performing sensing approaches to date opening the door toward integrated miniaturized all-carbon point-of-care devices. Addressing this opportunity, laser-scribed graphene(LSG) electrodes are demonstrated here as highly sensitive and reliable biosensor transducers in blood serum analysis. These flexible electrodes with large electrochemical surface areas were fabricated using a direct-write laser process on polyimide foils. A universal immobilization approach is established by anchoring 1-pyrenebutyric acid to the graphene and subsequently covalently attaching an aptamer against the coagulation factor thrombin as an exemplary bioreceptor to the carboxyl groups. The resulting biosensor displays extremely low detection limits of 1 pM in buffer and 5 pM in the complex matrix of serum.
KAUST Department:
Materials Science and Engineering Program
Citation:
Fenzl C, Nayak P, Hirsch T, Wolfbeis OS, Alshareef HN, et al. (2017) Laser-Scribed Graphene Electrodes for Aptamer-Based Biosensing. ACS Sensors 2: 616–620. Available: http://dx.doi.org/10.1021/acssensors.7b00066.
Publisher:
American Chemical Society (ACS)
Journal:
ACS Sensors
Issue Date:
25-Apr-2017
DOI:
10.1021/acssensors.7b00066
Type:
Article
ISSN:
2379-3694; 2379-3694
Sponsors:
Research reported in this publication was supported in part by funding from King Abdullah University of Science and Technology (KAUST).
Additional Links:
http://pubs.acs.org/doi/full/10.1021/acssensors.7b00066
Appears in Collections:
Articles; Materials Science and Engineering Program

Full metadata record

DC FieldValue Language
dc.contributor.authorFenzl, Christophen
dc.contributor.authorNayak, Pranatien
dc.contributor.authorHirsch, Thomasen
dc.contributor.authorWolfbeis, Otto S.en
dc.contributor.authorAlshareef, Husam N.en
dc.contributor.authorBaeumner, Antje J.en
dc.date.accessioned2017-06-21T06:42:08Z-
dc.date.available2017-06-21T06:42:08Z-
dc.date.issued2017-04-25en
dc.identifier.citationFenzl C, Nayak P, Hirsch T, Wolfbeis OS, Alshareef HN, et al. (2017) Laser-Scribed Graphene Electrodes for Aptamer-Based Biosensing. ACS Sensors 2: 616–620. Available: http://dx.doi.org/10.1021/acssensors.7b00066.en
dc.identifier.issn2379-3694en
dc.identifier.issn2379-3694en
dc.identifier.doi10.1021/acssensors.7b00066en
dc.identifier.urihttp://hdl.handle.net/10754/625082-
dc.description.abstractGraphene as a transducer material has produced some of the best performing sensing approaches to date opening the door toward integrated miniaturized all-carbon point-of-care devices. Addressing this opportunity, laser-scribed graphene(LSG) electrodes are demonstrated here as highly sensitive and reliable biosensor transducers in blood serum analysis. These flexible electrodes with large electrochemical surface areas were fabricated using a direct-write laser process on polyimide foils. A universal immobilization approach is established by anchoring 1-pyrenebutyric acid to the graphene and subsequently covalently attaching an aptamer against the coagulation factor thrombin as an exemplary bioreceptor to the carboxyl groups. The resulting biosensor displays extremely low detection limits of 1 pM in buffer and 5 pM in the complex matrix of serum.en
dc.description.sponsorshipResearch reported in this publication was supported in part by funding from King Abdullah University of Science and Technology (KAUST).en
dc.publisherAmerican Chemical Society (ACS)en
dc.relation.urlhttp://pubs.acs.org/doi/full/10.1021/acssensors.7b00066en
dc.subjectlaser-scribed grapheneen
dc.subjectbiosensoren
dc.subjectcarbon nanomaterialen
dc.subjectaptameren
dc.subjectimmobilizationen
dc.subjectelectrochemistryen
dc.titleLaser-Scribed Graphene Electrodes for Aptamer-Based Biosensingen
dc.typeArticleen
dc.contributor.departmentMaterials Science and Engineering Programen
dc.identifier.journalACS Sensorsen
dc.contributor.institutionInstitute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitaetsstrasse 31, 93053 Regensburg, Germanyen
kaust.authorNayak, Pranatien
kaust.authorAlshareef, Husam N.en
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