Anti-cancer agents in Saudi Arabian herbals revealed by automated high-content imaging

Handle URI:
http://hdl.handle.net/10754/625051
Title:
Anti-cancer agents in Saudi Arabian herbals revealed by automated high-content imaging
Authors:
Hajjar, Dina ( 0000-0003-3901-1172 ) ; Kremb, Stephan; Sioud, Salim; Emwas, Abdul-Hamid M.; Voolstra, Christian R. ( 0000-0003-4555-3795 ) ; Ravasi, Timothy ( 0000-0002-9950-465X )
Abstract:
Natural products have been used for medical applications since ancient times. Commonly, natural products are structurally complex chemical compounds that efficiently interact with their biological targets, making them useful drug candidates in cancer therapy. Here, we used cell-based phenotypic profiling and image-based high-content screening to study the mode of action and potential cellular targets of plants historically used in Saudi Arabia's traditional medicine. We compared the cytological profiles of fractions taken from Juniperus phoenicea (Arar), Anastatica hierochuntica (Kaff Maryam), and Citrullus colocynthis (Hanzal) with a set of reference compounds with established modes of action. Cluster analyses of the cytological profiles of the tested compounds suggested that these plants contain possible topoisomerase inhibitors that could be effective in cancer treatment. Using histone H2AX phosphorylation as a marker for DNA damage, we discovered that some of the compounds induced double-strand DNA breaks. Furthermore, chemical analysis of the active fraction isolated from Juniperus phoenicea revealed possible anti-cancer compounds. Our results demonstrate the usefulness of cell-based phenotypic screening of natural products to reveal their biological activities.
KAUST Department:
KAUST Environmental Epigenetics Research Program (KEEP); Red Sea Research Center (RSRC); Analytical Core Lab; Imaging and Characterization Core Lab
Citation:
Hajjar D, Kremb S, Sioud S, Emwas A-H, Voolstra CR, et al. (2017) Anti-cancer agents in Saudi Arabian herbals revealed by automated high-content imaging. PLOS ONE 12: e0177316. Available: http://dx.doi.org/10.1371/journal.pone.0177316.
Publisher:
Public Library of Science (PLoS)
Journal:
PLOS ONE
Issue Date:
13-Jun-2017
DOI:
10.1371/journal.pone.0177316
Type:
Article
ISSN:
1932-6203
Sponsors:
We thank Najeh Kharbatia, staff scientist, King Abdullah of Science and Technology (KAUST) Analytical Core Lab. We also thank Dr. Amal Aldhebiani (Assistant Professor of Plant Taxonomy in the Biological Sciences Department at King Abdulaziz University, Jeddah Saudi Arabia) for taxonomic plant identifications. The project was funded by: King Abdullah of Science and Technology (KAUST).
Additional Links:
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0177316
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Analytical Core Lab; Advanced Nanofabrication, Imaging and Characterization Core Lab

Full metadata record

DC FieldValue Language
dc.contributor.authorHajjar, Dinaen
dc.contributor.authorKremb, Stephanen
dc.contributor.authorSioud, Salimen
dc.contributor.authorEmwas, Abdul-Hamid M.en
dc.contributor.authorVoolstra, Christian R.en
dc.contributor.authorRavasi, Timothyen
dc.date.accessioned2017-06-19T09:21:45Z-
dc.date.available2017-06-19T09:21:45Z-
dc.date.issued2017-06-13en
dc.identifier.citationHajjar D, Kremb S, Sioud S, Emwas A-H, Voolstra CR, et al. (2017) Anti-cancer agents in Saudi Arabian herbals revealed by automated high-content imaging. PLOS ONE 12: e0177316. Available: http://dx.doi.org/10.1371/journal.pone.0177316.en
dc.identifier.issn1932-6203en
dc.identifier.doi10.1371/journal.pone.0177316en
dc.identifier.urihttp://hdl.handle.net/10754/625051-
dc.description.abstractNatural products have been used for medical applications since ancient times. Commonly, natural products are structurally complex chemical compounds that efficiently interact with their biological targets, making them useful drug candidates in cancer therapy. Here, we used cell-based phenotypic profiling and image-based high-content screening to study the mode of action and potential cellular targets of plants historically used in Saudi Arabia's traditional medicine. We compared the cytological profiles of fractions taken from Juniperus phoenicea (Arar), Anastatica hierochuntica (Kaff Maryam), and Citrullus colocynthis (Hanzal) with a set of reference compounds with established modes of action. Cluster analyses of the cytological profiles of the tested compounds suggested that these plants contain possible topoisomerase inhibitors that could be effective in cancer treatment. Using histone H2AX phosphorylation as a marker for DNA damage, we discovered that some of the compounds induced double-strand DNA breaks. Furthermore, chemical analysis of the active fraction isolated from Juniperus phoenicea revealed possible anti-cancer compounds. Our results demonstrate the usefulness of cell-based phenotypic screening of natural products to reveal their biological activities.en
dc.description.sponsorshipWe thank Najeh Kharbatia, staff scientist, King Abdullah of Science and Technology (KAUST) Analytical Core Lab. We also thank Dr. Amal Aldhebiani (Assistant Professor of Plant Taxonomy in the Biological Sciences Department at King Abdulaziz University, Jeddah Saudi Arabia) for taxonomic plant identifications. The project was funded by: King Abdullah of Science and Technology (KAUST).en
dc.publisherPublic Library of Science (PLoS)en
dc.relation.urlhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0177316en
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleAnti-cancer agents in Saudi Arabian herbals revealed by automated high-content imagingen
dc.typeArticleen
dc.contributor.departmentKAUST Environmental Epigenetics Research Program (KEEP)en
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.contributor.departmentAnalytical Core Laben
dc.contributor.departmentImaging and Characterization Core Laben
dc.identifier.journalPLOS ONEen
dc.eprint.versionPublisher's Version/PDFen
kaust.authorHajjar, Dinaen
kaust.authorKremb, Stephanen
kaust.authorSioud, Salimen
kaust.authorEmwas, Abdul-Hamid M.en
kaust.authorVoolstra, Christian R.en
kaust.authorRavasi, Timothyen
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