Thermoresponsive pegylated bubble liposome nanovectors for efficient siRNA delivery via endosomal escape

Handle URI:
http://hdl.handle.net/10754/625030
Title:
Thermoresponsive pegylated bubble liposome nanovectors for efficient siRNA delivery via endosomal escape
Authors:
Alamoudi, Kholod ( 0000-0002-7839-4746 ) ; Martins, Patricia; Croissant, Jonas G.; Patil, Sachin ( 0000-0003-4952-3120 ) ; Omar, Haneen; Khashab, Niveen M. ( 0000-0003-2728-0666 )
Abstract:
Improving the delivery of siRNA into cancer cells via bubble liposomes. Designing a thermoresponsive pegylated liposome through the introduction of ammonium bicarbonate salt into liposomes so as to control their endosomal escape for gene therapy.A sub-200 nm nanovector was fully characterized and examined for cellular uptake, cytotoxicity, endosomal escape and gene silencing.The siRNA-liposomes were internalized into cancer cells within 5 min and then released siRNAs in the cytosol prior to lysosomal degradation upon external temperature elevation. This was confirmed by confocal bioimaging and gene silencing reaching up to 90% and further demonstrated by the protein inhibition of both target genes.The thermoresponsiveness of ammonium bicarbonate containing liposomes enabled the rapid endosomal escape of the particles and resulted in an efficient gene silencing.
KAUST Department:
Advanced Membranes and Porous Materials Research Center
Citation:
Alamoudi K, Martins P, Croissant JG, Patil S, Omar H, et al. (2017) Thermoresponsive pegylated bubble liposome nanovectors for efficient siRNA delivery via endosomal escape. Nanomedicine. Available: http://dx.doi.org/10.2217/nnm-2017-0021.
Publisher:
Future Medicine Ltd
Journal:
Nanomedicine
Issue Date:
19-May-2017
DOI:
10.2217/nnm-2017-0021
Type:
Article
ISSN:
1743-5889; 1748-6963
Sponsors:
We gratefully acknowledge support from King Abdullah University of Science and Technology (KAUST). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.
Additional Links:
http://www.futuremedicine.com/doi/10.2217/nnm-2017-0021
Appears in Collections:
Articles; Advanced Membranes and Porous Materials Research Center

Full metadata record

DC FieldValue Language
dc.contributor.authorAlamoudi, Kholoden
dc.contributor.authorMartins, Patriciaen
dc.contributor.authorCroissant, Jonas G.en
dc.contributor.authorPatil, Sachinen
dc.contributor.authorOmar, Haneenen
dc.contributor.authorKhashab, Niveen M.en
dc.date.accessioned2017-06-14T12:17:35Z-
dc.date.available2017-06-14T12:17:35Z-
dc.date.issued2017-05-19en
dc.identifier.citationAlamoudi K, Martins P, Croissant JG, Patil S, Omar H, et al. (2017) Thermoresponsive pegylated bubble liposome nanovectors for efficient siRNA delivery via endosomal escape. Nanomedicine. Available: http://dx.doi.org/10.2217/nnm-2017-0021.en
dc.identifier.issn1743-5889en
dc.identifier.issn1748-6963en
dc.identifier.doi10.2217/nnm-2017-0021en
dc.identifier.urihttp://hdl.handle.net/10754/625030-
dc.description.abstractImproving the delivery of siRNA into cancer cells via bubble liposomes. Designing a thermoresponsive pegylated liposome through the introduction of ammonium bicarbonate salt into liposomes so as to control their endosomal escape for gene therapy.A sub-200 nm nanovector was fully characterized and examined for cellular uptake, cytotoxicity, endosomal escape and gene silencing.The siRNA-liposomes were internalized into cancer cells within 5 min and then released siRNAs in the cytosol prior to lysosomal degradation upon external temperature elevation. This was confirmed by confocal bioimaging and gene silencing reaching up to 90% and further demonstrated by the protein inhibition of both target genes.The thermoresponsiveness of ammonium bicarbonate containing liposomes enabled the rapid endosomal escape of the particles and resulted in an efficient gene silencing.en
dc.description.sponsorshipWe gratefully acknowledge support from King Abdullah University of Science and Technology (KAUST). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.en
dc.publisherFuture Medicine Ltden
dc.relation.urlhttp://www.futuremedicine.com/doi/10.2217/nnm-2017-0021en
dc.subjectLiposomeen
dc.subjectGene silencingen
dc.subjectNanomedicineen
dc.subjectSirna Deliveryen
dc.subjectLipoplexen
dc.subjectEndosomal Escapeen
dc.subjectBubble Generationen
dc.subjectMultidrug-resistant Cellen
dc.titleThermoresponsive pegylated bubble liposome nanovectors for efficient siRNA delivery via endosomal escapeen
dc.typeArticleen
dc.contributor.departmentAdvanced Membranes and Porous Materials Research Centeren
dc.identifier.journalNanomedicineen
kaust.authorAlamoudi, Kholoden
kaust.authorMartins, Patriciaen
kaust.authorCroissant, Jonas G.en
kaust.authorPatil, Sachinen
kaust.authorOmar, Haneenen
kaust.authorKhashab, Niveen M.en
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