The quest for epigenetic regulation underlying unisexual flower development in Cucumis melo

Handle URI:
http://hdl.handle.net/10754/624888
Title:
The quest for epigenetic regulation underlying unisexual flower development in Cucumis melo
Authors:
Latrasse, David; Rodriguez-Granados, Natalia Y.; Veluchamy, Alaguraj; Mariappan, Kiruthiga Gayathri; Bevilacqua, Claudia; Crapart, Nicolas; Camps, Celine; Sommard, Vivien; Raynaud, Cécile; Dogimont, Catherine; Boualem, Adnane; Benhamed, Moussa ( 0000-0002-4181-1702 ) ; Bendahmane, Abdelhafid
Abstract:
BackgroundMelon (Cucumis melo) is an important vegetable crop from the Cucurbitaceae family and a reference model specie for sex determination, fruit ripening and vascular fluxes studies. Nevertheless, the nature and role of its epigenome in gene expression regulation and more specifically in sex determination remains largely unknown.ResultsWe have investigated genome wide H3K27me3 and H3K9ac histone modifications and gene expression dynamics, in five melon organs. H3K9ac and H3K27me3 were mainly distributed along gene-rich regions and constrained to gene bodies. H3K9ac was preferentially located at the TSS, whereas H3K27me3 distributed uniformly from TSS to TES. As observed in other species, H3K9ac and H3K27me3 correlated with high and low gene expression levels, respectively. Comparative analyses of unisexual flowers pointed out sex-specific epigenetic states of TFs involved in ethylene response and flower development. Chip-qPCR analysis of laser dissected carpel and stamina primordia, revealed sex-specific histone modification of MADS-box genes. Using sex transition mutants, we demonstrated that the female promoting gene, CmACS11, represses the expression of the male promoting gene CmWIP1 via deposition of H3K27me3.ConclusionsOur findings reveal the organ-specific landscapes of H3K9ac and H3K27me3 in melon. Our results also provide evidence that the sex determination genes recruit histone modifiers to orchestrate unisexual flower development in monoecious species.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Latrasse D, Rodriguez-Granados NY, Veluchamy A, Mariappan KG, Bevilacqua C, et al. (2017) The quest for epigenetic regulation underlying unisexual flower development in Cucumis melo. Epigenetics & Chromatin 10. Available: http://dx.doi.org/10.1186/s13072-017-0132-6.
Publisher:
Springer Nature
Journal:
Epigenetics & Chromatin
Issue Date:
8-May-2017
DOI:
10.1186/s13072-017-0132-6
Type:
Article
ISSN:
1756-8935
Sponsors:
This work was supported by the Plant Biology and Breeding department in INRA, the grants Program Saclay Plant Sciences (SPS, ANR-10-LABX-40), Inititiative d’Excellence Paris-Saclay (Lidex-3P, ANR-11-IDEX-0003-02), L’Agence Nationale de la Recherche MELODY (ANR-11-BSV7-0024), and the European Research Council (ERC-SEXYPARTH).
Additional Links:
http://link.springer.com/article/10.1186/s13072-017-0132-6
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLatrasse, Daviden
dc.contributor.authorRodriguez-Granados, Natalia Y.en
dc.contributor.authorVeluchamy, Alagurajen
dc.contributor.authorMariappan, Kiruthiga Gayathrien
dc.contributor.authorBevilacqua, Claudiaen
dc.contributor.authorCrapart, Nicolasen
dc.contributor.authorCamps, Celineen
dc.contributor.authorSommard, Vivienen
dc.contributor.authorRaynaud, Cécileen
dc.contributor.authorDogimont, Catherineen
dc.contributor.authorBoualem, Adnaneen
dc.contributor.authorBenhamed, Moussaen
dc.contributor.authorBendahmane, Abdelhafiden
dc.date.accessioned2017-06-08T09:42:44Z-
dc.date.available2017-06-08T09:42:44Z-
dc.date.issued2017-05-08en
dc.identifier.citationLatrasse D, Rodriguez-Granados NY, Veluchamy A, Mariappan KG, Bevilacqua C, et al. (2017) The quest for epigenetic regulation underlying unisexual flower development in Cucumis melo. Epigenetics & Chromatin 10. Available: http://dx.doi.org/10.1186/s13072-017-0132-6.en
dc.identifier.issn1756-8935en
dc.identifier.doi10.1186/s13072-017-0132-6en
dc.identifier.urihttp://hdl.handle.net/10754/624888-
dc.description.abstractBackgroundMelon (Cucumis melo) is an important vegetable crop from the Cucurbitaceae family and a reference model specie for sex determination, fruit ripening and vascular fluxes studies. Nevertheless, the nature and role of its epigenome in gene expression regulation and more specifically in sex determination remains largely unknown.ResultsWe have investigated genome wide H3K27me3 and H3K9ac histone modifications and gene expression dynamics, in five melon organs. H3K9ac and H3K27me3 were mainly distributed along gene-rich regions and constrained to gene bodies. H3K9ac was preferentially located at the TSS, whereas H3K27me3 distributed uniformly from TSS to TES. As observed in other species, H3K9ac and H3K27me3 correlated with high and low gene expression levels, respectively. Comparative analyses of unisexual flowers pointed out sex-specific epigenetic states of TFs involved in ethylene response and flower development. Chip-qPCR analysis of laser dissected carpel and stamina primordia, revealed sex-specific histone modification of MADS-box genes. Using sex transition mutants, we demonstrated that the female promoting gene, CmACS11, represses the expression of the male promoting gene CmWIP1 via deposition of H3K27me3.ConclusionsOur findings reveal the organ-specific landscapes of H3K9ac and H3K27me3 in melon. Our results also provide evidence that the sex determination genes recruit histone modifiers to orchestrate unisexual flower development in monoecious species.en
dc.description.sponsorshipThis work was supported by the Plant Biology and Breeding department in INRA, the grants Program Saclay Plant Sciences (SPS, ANR-10-LABX-40), Inititiative d’Excellence Paris-Saclay (Lidex-3P, ANR-11-IDEX-0003-02), L’Agence Nationale de la Recherche MELODY (ANR-11-BSV7-0024), and the European Research Council (ERC-SEXYPARTH).en
dc.publisherSpringer Natureen
dc.relation.urlhttp://link.springer.com/article/10.1186/s13072-017-0132-6en
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleThe quest for epigenetic regulation underlying unisexual flower development in Cucumis meloen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalEpigenetics & Chromatinen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionInstitute of Plant Sciences Paris‑Saclay (IPS2), CNRS, INRA, University Paris‑Sud, University of Evry, University Paris‑Diderot, Sorbonne Paris‑Cite, University of Paris-Saclay, Batiment 630, 91405 Orsay, France.en
dc.contributor.institutionUMR 1313 Génétique Animale et Biologie Intégrative, Institut National de la Recherche Agronomique, 78350 Jouy‑en‑Josas, France.en
dc.contributor.institutionUR 1052, Unité de Génétique et d’Amélioration des Fruits et Légumes, INRA, BP94, 84143 Montfavet, France.en
kaust.authorVeluchamy, Alagurajen
kaust.authorMariappan, Kiruthiga Gayathrien
kaust.authorBenhamed, Moussaen
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