Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors, supplement to: Dineshram, R; Chandramouli, K; Ko, W K Ginger; Zhang, Huoming; Qian, Pei Yuan; Ravasi, Timothy; Thiyagarajan, Vengatesen (2016): Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors. Global Change Biology, 22(6), 2054-2068

Handle URI:
http://hdl.handle.net/10754/624161
Title:
Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors, supplement to: Dineshram, R; Chandramouli, K; Ko, W K Ginger; Zhang, Huoming; Qian, Pei Yuan; Ravasi, Timothy; Thiyagarajan, Vengatesen (2016): Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors. Global Change Biology, 22(6), 2054-2068
Authors:
Dineshram, R; Chandramouli, Kondethimmanahalli ( 0000-0002-0104-1993 ) ; Ko, W K Ginger; Zhang, Huoming ( 0000-0001-5416-0358 ) ; Qian, Pei Yuan; Ravasi, Timothy ( 0000-0002-9950-465X ) ; Thiyagarajan, Vengatesen
Abstract:
The metamorphosis of planktonic larvae of the Pacific oyster (Crassostrea gigas) underpins their complex life-history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by different anthropogenically induced climate change-related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae might be affected in a future ocean, we examined changes in the proteome of metamorphosing larvae under multiple stressors: decreased pH (pH 7.4), increased temperature (30 °C), and reduced salinity (15 psu). Quantitative protein expression profiling using iTRAQ-LC-MS/MS identified more than 1300 proteins. Decreased pH had a negative effect on metamorphosis by down-regulating several proteins involved in energy production, metabolism, and protein synthesis. However, warming switched on these down-regulated pathways at pH 7.4. Under multiple stressors, cell signaling, energy production, growth, and developmental pathways were up-regulated, although metamorphosis was still reduced. Despite the lack of lethal effects, significant physiological responses to both individual and interacting climate change related stressors were observed at proteome level. The metamorphosing larvae of the C. gigas population in the Yellow Sea appear to have adequate phenotypic plasticity at the proteome level to survive in future coastal oceans, but with developmental and physiological costs.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Biosciences Core Lab
Citation:
Dineshram, R., Chandramouli, K., Ko, W. K. G., Zhang, H., Qian, P. Y., Ravasi, T., & Thiyagarajan, V. (2016). Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors, supplement to: Dineshram, R; Chandramouli, K; Ko, W K Ginger; Zhang, Huoming; Qian, Pei Yuan; Ravasi, Timothy; Thiyagarajan, Vengatesen (2016): Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors. Global Change Biology, 22(6), 2054-2068 [Data set]. PANGAEA - Data Publisher for Earth & Environmental Science. https://doi.org/10.1594/pangaea.867318
Publisher:
PANGAEA - Data Publisher for Earth & Environmental Science
Issue Date:
2016
DOI:
10.1594/PANGAEA.867318
Type:
Dataset
Is Documented By:
URL:https://cran.r-project.org/package=seacarb
Is Supplement To:
Dineshram R, Chandramouli K, Ko GWK, Zhang H, Qian P-Y, et al. (2016) Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors. Global Change Biology 22: 2054–2068. Available: http://dx.doi.org/10.1111/gcb.13249.; DOI:10.1111/gcb.13249; HANDLE:http://hdl.handle.net/10754/621623
Appears in Collections:
Biosciences Core Lab; Datasets; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributorYang, Yanen
dc.contributor.authorDineshram, Ren
dc.contributor.authorChandramouli, Kondethimmanahallien
dc.contributor.authorKo, W K Gingeren
dc.contributor.authorZhang, Huomingen
dc.contributor.authorQian, Pei Yuanen
dc.contributor.authorRavasi, Timothyen
dc.contributor.authorThiyagarajan, Vengatesenen
dc.coverage.spatialgeoLocationPoint: 36.06667 120.36667en
dc.date.accessioned2017-06-06T08:02:23Z-
dc.date.available2017-06-06T08:02:23Z-
dc.date.issued2016en
dc.identifier.citationDineshram, R., Chandramouli, K., Ko, W. K. G., Zhang, H., Qian, P. Y., Ravasi, T., & Thiyagarajan, V. (2016). Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors, supplement to: Dineshram, R; Chandramouli, K; Ko, W K Ginger; Zhang, Huoming; Qian, Pei Yuan; Ravasi, Timothy; Thiyagarajan, Vengatesen (2016): Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors. Global Change Biology, 22(6), 2054-2068 [Data set]. PANGAEA - Data Publisher for Earth & Environmental Science. https://doi.org/10.1594/pangaea.867318en
dc.identifier.doi10.1594/PANGAEA.867318en
dc.identifier.urihttp://hdl.handle.net/10754/624161-
dc.description.abstractThe metamorphosis of planktonic larvae of the Pacific oyster (Crassostrea gigas) underpins their complex life-history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by different anthropogenically induced climate change-related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae might be affected in a future ocean, we examined changes in the proteome of metamorphosing larvae under multiple stressors: decreased pH (pH 7.4), increased temperature (30 °C), and reduced salinity (15 psu). Quantitative protein expression profiling using iTRAQ-LC-MS/MS identified more than 1300 proteins. Decreased pH had a negative effect on metamorphosis by down-regulating several proteins involved in energy production, metabolism, and protein synthesis. However, warming switched on these down-regulated pathways at pH 7.4. Under multiple stressors, cell signaling, energy production, growth, and developmental pathways were up-regulated, although metamorphosis was still reduced. Despite the lack of lethal effects, significant physiological responses to both individual and interacting climate change related stressors were observed at proteome level. The metamorphosing larvae of the C. gigas population in the Yellow Sea appear to have adequate phenotypic plasticity at the proteome level to survive in future coastal oceans, but with developmental and physiological costs.en
dc.formattext/tab-separated-valuesen
dc.format.extent269779 data pointsen
dc.language.isoengen
dc.publisherPANGAEA - Data Publisher for Earth & Environmental Scienceen
dc.rightsCreative Commons Attribution 3.0 Unported (CC-BY)en
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/en
dc.titleQuantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors, supplement to: Dineshram, R; Chandramouli, K; Ko, W K Ginger; Zhang, Huoming; Qian, Pei Yuan; Ravasi, Timothy; Thiyagarajan, Vengatesen (2016): Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors. Global Change Biology, 22(6), 2054-2068en
dc.typeDataseten
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBiosciences Core Laben
kaust.authorChandramouli, Kondethimmanahallien
kaust.authorZhang, Huomingen
kaust.authorRavasi, Timothyen
dc.type.resourceSupplementary Dataseten
dc.relation.isDocumentedByURL:https://cran.r-project.org/package=seacarben
dc.relation.isSupplementToDineshram R, Chandramouli K, Ko GWK, Zhang H, Qian P-Y, et al. (2016) Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors. Global Change Biology 22: 2054–2068. Available: http://dx.doi.org/10.1111/gcb.13249.en
dc.relation.isSupplementToDOI:10.1111/gcb.13249en
dc.relation.isSupplementToHANDLE:http://hdl.handle.net/10754/621623en
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