Supplementary Material for: Herboxidiene triggers splicing repression and abiotic stress responses in plants

Handle URI:
http://hdl.handle.net/10754/624149
Title:
Supplementary Material for: Herboxidiene triggers splicing repression and abiotic stress responses in plants
Authors:
Alshareef, Sahar ( 0000-0002-0377-1113 ) ; Ling, Yu ( 0000-0002-8067-523X ) ; Butt, Haroon; Mariappan, Kiruthiga; Benhamed, Moussa ( 0000-0002-4181-1702 ) ; Mahfouz, Magdy
Abstract:
Abstract Background Constitutive and alternative splicing of pre-mRNAs from multiexonic genes controls the diversity of the proteome; these precisely regulated processes also fine-tune responses to cues related to growth, development, and stresses. Small-molecule inhibitors that perturb splicing provide invaluable tools for use as chemical probes to uncover the molecular underpinnings of splicing regulation and as potential anticancer compounds. Results Here, we show that herboxidiene (GEX1A) inhibits both constitutive and alternative splicing. Moreover, GEX1A activates genome-wide transcriptional patterns involved in abiotic stress responses in plants. GEX1A treatment -activated ABA-inducible promoters, and led to stomatal closure. Interestingly, GEX1A and pladienolide B (PB) elicited similar cellular changes, including alterations in the patterns of transcription and splicing, suggesting that these compounds might target the same spliceosome complex in plant cells. Conclusions Our study establishes GEX1A as a potent splicing inhibitor in plants that can be used to probe the assembly, dynamics, and molecular functions of the spliceosome and to study the interplay between splicing stress and abiotic stresses, as well as having potential biotechnological applications.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Plant Science Program; Desert Agriculture Initiative
Citation:
AlShareef, S., Ling, Y., Butt, H., Kiruthiga Mariappan, Benhamed, M., & Magdy Mahfouz. (2017). Herboxidiene triggers splicing repression and abiotic stress responses in plants. Figshare. https://doi.org/10.6084/m9.figshare.c.3727597
Publisher:
Figshare
Issue Date:
2017
DOI:
10.6084/m9.figshare.c.3727597
Type:
Dataset
Is Supplement To:
AlShareef S, Ling Y, Butt H, Mariappan KG, Benhamed M, et al. (2017) Herboxidiene triggers splicing repression and abiotic stress responses in plants. BMC Genomics 18. Available: http://dx.doi.org/10.1186/s12864-017-3656-z.; DOI:10.1186/s12864-017-3656-z; HANDLE:http://hdl.handle.net/10754/623088
Appears in Collections:
Bioscience Program; Datasets; Plant Science Program; Desert Agriculture Initiative; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorAlshareef, Saharen
dc.contributor.authorLing, Yuen
dc.contributor.authorButt, Haroonen
dc.contributor.authorMariappan, Kiruthigaen
dc.contributor.authorBenhamed, Moussaen
dc.contributor.authorMahfouz, Magdyen
dc.date.accessioned2017-06-06T07:44:34Z-
dc.date.available2017-06-06T07:44:34Z-
dc.date.created2017-03-28en
dc.date.issued2017en
dc.identifier.citationAlShareef, S., Ling, Y., Butt, H., Kiruthiga Mariappan, Benhamed, M., & Magdy Mahfouz. (2017). Herboxidiene triggers splicing repression and abiotic stress responses in plants. Figshare. https://doi.org/10.6084/m9.figshare.c.3727597en
dc.identifier.doi10.6084/m9.figshare.c.3727597en
dc.identifier.urihttp://hdl.handle.net/10754/624149-
dc.description.abstractAbstract Background Constitutive and alternative splicing of pre-mRNAs from multiexonic genes controls the diversity of the proteome; these precisely regulated processes also fine-tune responses to cues related to growth, development, and stresses. Small-molecule inhibitors that perturb splicing provide invaluable tools for use as chemical probes to uncover the molecular underpinnings of splicing regulation and as potential anticancer compounds. Results Here, we show that herboxidiene (GEX1A) inhibits both constitutive and alternative splicing. Moreover, GEX1A activates genome-wide transcriptional patterns involved in abiotic stress responses in plants. GEX1A treatment -activated ABA-inducible promoters, and led to stomatal closure. Interestingly, GEX1A and pladienolide B (PB) elicited similar cellular changes, including alterations in the patterns of transcription and splicing, suggesting that these compounds might target the same spliceosome complex in plant cells. Conclusions Our study establishes GEX1A as a potent splicing inhibitor in plants that can be used to probe the assembly, dynamics, and molecular functions of the spliceosome and to study the interplay between splicing stress and abiotic stresses, as well as having potential biotechnological applications.en
dc.publisherFigshareen
dc.rightsCC BYen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectBiophysicsen
dc.subjectBiochemistryen
dc.subjectCell Biologyen
dc.subjectGeneticsen
dc.subjectMolecular Biologyen
dc.subjectEcologyen
dc.subjectDevelopmental Biologyen
dc.titleSupplementary Material for: Herboxidiene triggers splicing repression and abiotic stress responses in plantsen
dc.typeDataseten
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentPlant Science Programen
dc.contributor.departmentDesert Agriculture Initiativeen
kaust.authorAlshareef, Saharen
kaust.authorLing, Yuen
kaust.authorButt, Haroonen
kaust.authorMariappan, Kiruthigaen
kaust.authorBenhamed, Moussaen
dc.type.resourceCollectionen
dc.relation.isSupplementToAlShareef S, Ling Y, Butt H, Mariappan KG, Benhamed M, et al. (2017) Herboxidiene triggers splicing repression and abiotic stress responses in plants. BMC Genomics 18. Available: http://dx.doi.org/10.1186/s12864-017-3656-z.en
dc.relation.isSupplementToDOI:10.1186/s12864-017-3656-zen
dc.relation.isSupplementToHANDLE:http://hdl.handle.net/10754/623088en
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