Supplementary Material for: Detection of antibiotic resistance in probiotics of dietary supplements

Handle URI:
http://hdl.handle.net/10754/624145
Title:
Supplementary Material for: Detection of antibiotic resistance in probiotics of dietary supplements
Authors:
Wong, Aloysius Tze ( 0000-0002-9105-5845 ) ; Ngu, Davey; Dan, Lydia; Ooi, Amanda Siok Lee; Lim, Renee
Abstract:
Abstract Background Probiotics are live microorganisms that confer nutrition- and health-promoting benefits if consumed in adequate amounts. Concomitant with the demand for natural approaches to maintaining health is an increase in inclusion of probiotics in food and health products. Since probiotic bacteria act as reservoir for antibiotic resistant determinants, the transfer of these genes to pathogens sharing the same intestinal habitat is thus conceivable considering the fact that dietary supplements contain high amounts of often heterogeneous populations of probiotics. Such events can confer pathogens protection against commonly-used drugs. Despite numerous reports of antibiotic resistant probiotics in food and biological sources, the antibiogram of probiotics from dietary supplements remained elusive. Findings Here, we screened five commercially available dietary supplements for resistance towards antibiotics of different classes. Probiotics of all batches of products were resistant towards vancomycin while batch-dependent resistance towards streptomycin, aztreonam, gentamycin and/or ciprofloxacin antibiotics was detected for probiotics of brands Bi and Bn, Bg, and L. Isolates of brand Cn was also resistant towards gentamycin, streptomycin and ciprofloxacin antibiotics. Additionally, we also report a discrepancy between the enumerated viable bacteria amounts and the claims of the manufacturers. Conclusions This short report has highlighted the present of antibiotic resistance in probiotic bacteria from dietary supplements and therefore serves as a platform for further screenings and for in-depth characterization of the resistant determinants and the molecular machinery that confers the resistance.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program
Citation:
Wong, A., Ngu, D., Dan, L., Ooi, A., & Lim, R. (2015). Detection of antibiotic resistance in probiotics of dietary supplements. Figshare. https://doi.org/10.6084/m9.figshare.c.3697372
Publisher:
Figshare
Issue Date:
2015
DOI:
10.6084/m9.figshare.c.3697372
Type:
Dataset
Is Supplement To:
Detection of antibiotic resistance in probiotics of dietary supplements 2015, 14 (1) Nutrition Journal; DOI:10.1186/s12937-015-0084-2; HANDLE:http://hdl.handle.net/10754/578844
Appears in Collections:
Bioscience Program; Datasets; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorWong, Aloysius Tzeen
dc.contributor.authorNgu, Daveyen
dc.contributor.authorDan, Lydiaen
dc.contributor.authorOoi, Amanda Siok Leeen
dc.contributor.authorLim, Reneeen
dc.date.accessioned2017-06-06T07:44:33Z-
dc.date.available2017-06-06T07:44:33Z-
dc.date.created2017-02-21en
dc.date.issued2015en
dc.identifier.citationWong, A., Ngu, D., Dan, L., Ooi, A., & Lim, R. (2015). Detection of antibiotic resistance in probiotics of dietary supplements. Figshare. https://doi.org/10.6084/m9.figshare.c.3697372en
dc.identifier.doi10.6084/m9.figshare.c.3697372en
dc.identifier.urihttp://hdl.handle.net/10754/624145-
dc.description.abstractAbstract Background Probiotics are live microorganisms that confer nutrition- and health-promoting benefits if consumed in adequate amounts. Concomitant with the demand for natural approaches to maintaining health is an increase in inclusion of probiotics in food and health products. Since probiotic bacteria act as reservoir for antibiotic resistant determinants, the transfer of these genes to pathogens sharing the same intestinal habitat is thus conceivable considering the fact that dietary supplements contain high amounts of often heterogeneous populations of probiotics. Such events can confer pathogens protection against commonly-used drugs. Despite numerous reports of antibiotic resistant probiotics in food and biological sources, the antibiogram of probiotics from dietary supplements remained elusive. Findings Here, we screened five commercially available dietary supplements for resistance towards antibiotics of different classes. Probiotics of all batches of products were resistant towards vancomycin while batch-dependent resistance towards streptomycin, aztreonam, gentamycin and/or ciprofloxacin antibiotics was detected for probiotics of brands Bi and Bn, Bg, and L. Isolates of brand Cn was also resistant towards gentamycin, streptomycin and ciprofloxacin antibiotics. Additionally, we also report a discrepancy between the enumerated viable bacteria amounts and the claims of the manufacturers. Conclusions This short report has highlighted the present of antibiotic resistance in probiotic bacteria from dietary supplements and therefore serves as a platform for further screenings and for in-depth characterization of the resistant determinants and the molecular machinery that confers the resistance.en
dc.publisherFigshareen
dc.rightsCC BYen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectSpace Scienceen
dc.subjectMedicineen
dc.subjectMicrobiologyen
dc.subjectPharmacologyen
dc.subjectBiotechnologyen
dc.subjectEcologyen
dc.subjectSociologyen
dc.subjectCanceren
dc.subjectInfectious Diseasesen
dc.subjectComputational Biologyen
dc.titleSupplementary Material for: Detection of antibiotic resistance in probiotics of dietary supplementsen
dc.typeDataseten
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
kaust.authorWong, Aloysius Tzeen
kaust.authorOoi, Amanda Siok Leeen
dc.type.resourceCollectionen
dc.relation.isSupplementToDetection of antibiotic resistance in probiotics of dietary supplements 2015, 14 (1) Nutrition Journalen
dc.relation.isSupplementToDOI:10.1186/s12937-015-0084-2en
dc.relation.isSupplementToHANDLE:http://hdl.handle.net/10754/578844en
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