Supplementary Material for: Transcriptome and proteome dynamics in larvae of the barnacle Balanus Amphitrite from the Red Sea

Handle URI:
http://hdl.handle.net/10754/624137
Title:
Supplementary Material for: Transcriptome and proteome dynamics in larvae of the barnacle Balanus Amphitrite from the Red Sea
Authors:
Chandramouli, Kondethimmanahalli ( 0000-0002-0104-1993 ) ; Al-Aqeel, Sarah ( 0000-0002-4918-5849 ) ; Ryu, Tae Woo; Zhang, Huoming ( 0000-0001-5416-0358 ) ; Seridi, Loqmane; Ghosheh, Yanal ( 0000-0002-3733-2098 ) ; Qian, Pei-Yuan; Ravasi, Timothy ( 0000-0002-9950-465X )
Abstract:
Abstract Background The barnacle Balanus amphitrite is widely distributed in marine shallow and tidal waters, and has significant economic and ecological importance. Nauplii, the first larval stage of most crustaceans, are extremely abundant in the marine zooplankton. However, a lack of genome information has hindered elucidation of the molecular mechanisms of development, settlement and survival strategies in extreme marine environments. We sequenced and constructed the genome dataset for nauplii to obtain comprehensive larval genetic information. We also investigated iTRAQ-based protein expression patterns to reveal the molecular basis of nauplii development, and to gain information on larval survival strategies in the Red Sea marine environment. Results A nauplii larval transcript dataset, containing 92,117 predicted open reading frames (ORFs), was constructed and used as a reference for the proteome analysis. Genes related to translation, oxidative phosphorylation and cytoskeletal development were highly abundant. We observed remarkable plasticity in the proteome of Red Sea larvae. The proteins associated with development, stress responses and osmoregulation showed the most significant differences between the two larval populations studied. The synergistic overexpression of heat shock and osmoregulatory proteins may facilitate larval survival in intertidal habitats or in extreme environments. Conclusions We presented, for the first time, comprehensive transcriptome and proteome datasets for Red Sea nauplii. The datasets provide a foundation for future investigations focused on the survival mechanisms of other crustaceans in extreme marine environments.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Chemical and Biological Engineering Program; Biosciences Core Lab; Computer Science Program
Citation:
Kondethimmanahalli Chandramouli, Al-Aqeel, S., Taewoo Ryu, Huoming Zhang, Loqmane Seridi, Yanal Ghosheh, … Ravasi, T. (2015). Transcriptome and proteome dynamics in larvae of the barnacle Balanus Amphitrite from the Red Sea. Figshare. https://doi.org/10.6084/m9.figshare.c.3629591
Publisher:
Figshare
Issue Date:
2015
DOI:
10.6084/m9.figshare.c.3629591
Type:
Dataset
Is Supplement To:
Transcriptome and proteome dynamics in larvae of the barnacle Balanus Amphitrite from the Red Sea 2015, 16 (1) BMC Genomics; DOI:10.1186/s12864-015-2262-1; HANDLE:http://hdl.handle.net/10754/584004
Appears in Collections:
Computer Science Program; Chemical and Biological Engineering Program; Datasets; Bioscience Core Lab; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorChandramouli, Kondethimmanahallien
dc.contributor.authorAl-Aqeel, Sarahen
dc.contributor.authorRyu, Tae Wooen
dc.contributor.authorZhang, Huomingen
dc.contributor.authorSeridi, Loqmaneen
dc.contributor.authorGhosheh, Yanalen
dc.contributor.authorQian, Pei-Yuanen
dc.contributor.authorRavasi, Timothyen
dc.date.accessioned2017-06-06T07:44:32Z-
dc.date.available2017-06-06T07:44:32Z-
dc.date.created2016-12-15en
dc.date.issued2015en
dc.identifier.citationKondethimmanahalli Chandramouli, Al-Aqeel, S., Taewoo Ryu, Huoming Zhang, Loqmane Seridi, Yanal Ghosheh, … Ravasi, T. (2015). Transcriptome and proteome dynamics in larvae of the barnacle Balanus Amphitrite from the Red Sea. Figshare. https://doi.org/10.6084/m9.figshare.c.3629591en
dc.identifier.doi10.6084/m9.figshare.c.3629591en
dc.identifier.urihttp://hdl.handle.net/10754/624137-
dc.description.abstractAbstract Background The barnacle Balanus amphitrite is widely distributed in marine shallow and tidal waters, and has significant economic and ecological importance. Nauplii, the first larval stage of most crustaceans, are extremely abundant in the marine zooplankton. However, a lack of genome information has hindered elucidation of the molecular mechanisms of development, settlement and survival strategies in extreme marine environments. We sequenced and constructed the genome dataset for nauplii to obtain comprehensive larval genetic information. We also investigated iTRAQ-based protein expression patterns to reveal the molecular basis of nauplii development, and to gain information on larval survival strategies in the Red Sea marine environment. Results A nauplii larval transcript dataset, containing 92,117 predicted open reading frames (ORFs), was constructed and used as a reference for the proteome analysis. Genes related to translation, oxidative phosphorylation and cytoskeletal development were highly abundant. We observed remarkable plasticity in the proteome of Red Sea larvae. The proteins associated with development, stress responses and osmoregulation showed the most significant differences between the two larval populations studied. The synergistic overexpression of heat shock and osmoregulatory proteins may facilitate larval survival in intertidal habitats or in extreme environments. Conclusions We presented, for the first time, comprehensive transcriptome and proteome datasets for Red Sea nauplii. The datasets provide a foundation for future investigations focused on the survival mechanisms of other crustaceans in extreme marine environments.en
dc.publisherFigshareen
dc.rightsCC BYen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectBiophysicsen
dc.subjectGeneticsen
dc.subjectEcologyen
dc.subjectMarine Biologyen
dc.subjectInorganic Chemistryen
dc.titleSupplementary Material for: Transcriptome and proteome dynamics in larvae of the barnacle Balanus Amphitrite from the Red Seaen
dc.typeDataseten
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentChemical and Biological Engineering Programen
dc.contributor.departmentBiosciences Core Laben
dc.contributor.departmentComputer Science Programen
kaust.authorChandramouli, Kondethimmanahallien
kaust.authorAl-Aqeel, Sarahen
kaust.authorRyu, Tae Wooen
kaust.authorZhang, Huomingen
kaust.authorSeridi, Loqmaneen
kaust.authorGhosheh, Yanalen
kaust.authorRavasi, Timothyen
dc.type.resourceCollectionen
dc.relation.isSupplementToTranscriptome and proteome dynamics in larvae of the barnacle Balanus Amphitrite from the Red Sea 2015, 16 (1) BMC Genomicsen
dc.relation.isSupplementToDOI:10.1186/s12864-015-2262-1en
dc.relation.isSupplementToHANDLE:http://hdl.handle.net/10754/584004en
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