Supplementary Material for: CRISPR/Cas9-mediated viral interference in plants

Handle URI:
http://hdl.handle.net/10754/624131
Title:
Supplementary Material for: CRISPR/Cas9-mediated viral interference in plants
Authors:
Ali, Zahir ( 0000-0002-7814-8908 ) ; Abulfaraj, Aala Abdulaziz Hussien; Idris, Ali; Ali, Shakila; Tashkandi, Manal; Mahfouz, Magdy
Abstract:
Abstract Background The CRISPR/Cas9 system provides bacteria and archaea with molecular immunity against invading phages and conjugative plasmids. Recently, CRISPR/Cas9 has been used for targeted genome editing in diverse eukaryotic species. Results In this study, we investigate whether the CRISPR/Cas9 system could be used in plants to confer molecular immunity against DNA viruses. We deliver sgRNAs specific for coding and non-coding sequences of tomato yellow leaf curl virus (TYLCV) into Nicotiana benthamiana plants stably overexpressing the Cas9 endonuclease, and subsequently challenge these plants with TYLCV. Our data demonstrate that the CRISPR/Cas9 system targeted TYLCV for degradation and introduced mutations at the target sequences. All tested sgRNAs exhibit interference activity, but those targeting the stem-loop sequence within the TYLCV origin of replication in the intergenic region (IR) are the most effective. N. benthamiana plants expressing CRISPR/Cas9 exhibit delayed or reduced accumulation of viral DNA, abolishing or significantly attenuating symptoms of infection. Moreover, this system could simultaneously target multiple DNA viruses. Conclusions These data establish the efficacy of the CRISPR/Cas9 system for viral interference in plants, thereby extending the utility of this technology and opening the possibility of producing plants resistant to multiple viral infections.
KAUST Department:
Center for Desert Agriculture; Bioscience Program
Citation:
Ali, Z., Aala Abulfaraj, Idris, A., Ali, S., Tashkandi, M., & Magdy Mahfouz. (2015). CRISPR/Cas9-mediated viral interference in plants. Figshare. https://doi.org/10.6084/m9.figshare.c.3612878
Publisher:
Figshare
Issue Date:
2015
DOI:
10.6084/m9.figshare.c.3612878
Type:
Dataset
Is Supplement To:
CRISPR/Cas9-mediated viral interference in plants 2015, 16 (1) Genome Biology; DOI:10.1186/s13059-015-0799-6; HANDLE:http://hdl.handle.net/10754/582543
Appears in Collections:
Bioscience Program; Center for Desert Agriculture; Datasets

Full metadata record

DC FieldValue Language
dc.contributor.authorAli, Zahiren
dc.contributor.authorAbulfaraj, Aala Abdulaziz Hussienen
dc.contributor.authorIdris, Alien
dc.contributor.authorAli, Shakilaen
dc.contributor.authorTashkandi, Manalen
dc.contributor.authorMahfouz, Magdyen
dc.date.accessioned2017-06-06T07:44:32Z-
dc.date.available2017-06-06T07:44:32Z-
dc.date.created2016-12-14en
dc.date.issued2015en
dc.identifier.citationAli, Z., Aala Abulfaraj, Idris, A., Ali, S., Tashkandi, M., & Magdy Mahfouz. (2015). CRISPR/Cas9-mediated viral interference in plants. Figshare. https://doi.org/10.6084/m9.figshare.c.3612878en
dc.identifier.doi10.6084/m9.figshare.c.3612878en
dc.identifier.urihttp://hdl.handle.net/10754/624131-
dc.description.abstractAbstract Background The CRISPR/Cas9 system provides bacteria and archaea with molecular immunity against invading phages and conjugative plasmids. Recently, CRISPR/Cas9 has been used for targeted genome editing in diverse eukaryotic species. Results In this study, we investigate whether the CRISPR/Cas9 system could be used in plants to confer molecular immunity against DNA viruses. We deliver sgRNAs specific for coding and non-coding sequences of tomato yellow leaf curl virus (TYLCV) into Nicotiana benthamiana plants stably overexpressing the Cas9 endonuclease, and subsequently challenge these plants with TYLCV. Our data demonstrate that the CRISPR/Cas9 system targeted TYLCV for degradation and introduced mutations at the target sequences. All tested sgRNAs exhibit interference activity, but those targeting the stem-loop sequence within the TYLCV origin of replication in the intergenic region (IR) are the most effective. N. benthamiana plants expressing CRISPR/Cas9 exhibit delayed or reduced accumulation of viral DNA, abolishing or significantly attenuating symptoms of infection. Moreover, this system could simultaneously target multiple DNA viruses. Conclusions These data establish the efficacy of the CRISPR/Cas9 system for viral interference in plants, thereby extending the utility of this technology and opening the possibility of producing plants resistant to multiple viral infections.en
dc.publisherFigshareen
dc.rightsCC BYen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectMicrobiologyen
dc.subjectGeneticsen
dc.subjectMolecular Biologyen
dc.subjectEvolutionary Biologyen
dc.subjectEcologyen
dc.subjectInfectious Diseasesen
dc.subjectPlant Biologyen
dc.subjectVirologyen
dc.subjectComputational Biologyen
dc.titleSupplementary Material for: CRISPR/Cas9-mediated viral interference in plantsen
dc.typeDataseten
dc.contributor.departmentCenter for Desert Agricultureen
dc.contributor.departmentBioscience Programen
kaust.authorAli, Zahiren
kaust.authorAbulfaraj, Aala Abdulaziz Hussienen
kaust.authorTashkandi, Manalen
dc.type.resourceCollectionen
dc.relation.isSupplementToCRISPR/Cas9-mediated viral interference in plants 2015, 16 (1) Genome Biologyen
dc.relation.isSupplementToDOI:10.1186/s13059-015-0799-6en
dc.relation.isSupplementToHANDLE:http://hdl.handle.net/10754/582543en
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