Supplementary Material for: Cytotoxicity and intracellular dissolution of nickel nanowires

Handle URI:
http://hdl.handle.net/10754/624123
Title:
Supplementary Material for: Cytotoxicity and intracellular dissolution of nickel nanowires
Authors:
Perez, Jose E.; Contreras, Maria F. ( 0000-0001-6239-5325 ) ; Vidal, Enrique Vilanova; Felix Servin, Laura P.; Margineanu, Michael B. ( 0000-0002-9836-1802 ) ; Luongo, Giovanni; Porter, Alexandra E.; Dunlop, Iain E.; Ravasi, Timothy ( 0000-0002-9950-465X ) ; Kosel, Jürgen ( 0000-0002-8998-8275 )
Abstract:
<p>The assessment of cytotoxicity of nanostructures is a fundamental step for their development as biomedical tools. As widely used nanostructures, nickel nanowires (Ni NWs) seem promising candidates for such applications. In this work, Ni NWs were synthesized and then characterized using vibrating sample magnetometry, energy dispersive X-Ray analysis, and electron microscopy. After exposure to the NWs, cytotoxicity was evaluated in terms of cell viability, cell membrane damage, and induced apoptosis/necrosis on the model human cell line HCT 116. The influence of NW to cell ratio (10:1 to 1000:1) and exposure times up to 72 hours was analyzed for Ni NWs of 5.4 μm in length, as well as for Ni ions. The results show that cytotoxicity markedly increases past 24 hours of incubation. Cellular uptake of NWs takes place through the phagocytosis pathway, with a fraction of the dose of NWs dissolved inside the cells. Cell death results from a combination of apoptosis and necrosis, where the latter is the outcome of the secondary necrosis pathway. The cytotoxicity of Ni ions and Ni NWs dissolution studies suggest a synergistic toxicity between NW aspect ratio and dissolved Ni, with the cytotoxic effects markedly increasing after 24 hours of incubation.</p>
KAUST Department:
Bioscience Program; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division; Chemical and Biological Engineering Program; Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Perez, J. E., Contreras, M. F., Vilanova, E., Felix, L. P., Margineanu, M. B., Luongo, G., … Kosel, J. (2016). Cytotoxicity and intracellular dissolution of nickel nanowires. Figshare. https://doi.org/10.6084/m9.figshare.c.2169857
Publisher:
Figshare
Issue Date:
2016
DOI:
10.6084/M9.FIGSHARE.C.2169857
Type:
Dataset
Is Supplement To:
Cytotoxicity and intracellular dissolution of nickel nanowires 2015:1 Nanotoxicology; DOI:10.3109/17435390.2015.1132343; HANDLE:http://hdl.handle.net/10754/592811
Appears in Collections:
Bioscience Program; Chemical and Biological Engineering Program; Datasets; Biological and Environmental Sciences and Engineering (BESE) Division; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorPerez, Jose E.en
dc.contributor.authorContreras, Maria F.en
dc.contributor.authorVidal, Enrique Vilanovaen
dc.contributor.authorFelix Servin, Laura P.en
dc.contributor.authorMargineanu, Michael B.en
dc.contributor.authorLuongo, Giovannien
dc.contributor.authorPorter, Alexandra E.en
dc.contributor.authorDunlop, Iain E.en
dc.contributor.authorRavasi, Timothyen
dc.contributor.authorKosel, Jürgenen
dc.date.accessioned2017-06-06T07:44:31Z-
dc.date.available2017-06-06T07:44:31Z-
dc.date.created2015-12-23en
dc.date.issued2016en
dc.identifier.citationPerez, J. E., Contreras, M. F., Vilanova, E., Felix, L. P., Margineanu, M. B., Luongo, G., … Kosel, J. (2016). Cytotoxicity and intracellular dissolution of nickel nanowires. Figshare. https://doi.org/10.6084/m9.figshare.c.2169857en
dc.identifier.doi10.6084/M9.FIGSHARE.C.2169857en
dc.identifier.urihttp://hdl.handle.net/10754/624123-
dc.description.abstract<p>The assessment of cytotoxicity of nanostructures is a fundamental step for their development as biomedical tools. As widely used nanostructures, nickel nanowires (Ni NWs) seem promising candidates for such applications. In this work, Ni NWs were synthesized and then characterized using vibrating sample magnetometry, energy dispersive X-Ray analysis, and electron microscopy. After exposure to the NWs, cytotoxicity was evaluated in terms of cell viability, cell membrane damage, and induced apoptosis/necrosis on the model human cell line HCT 116. The influence of NW to cell ratio (10:1 to 1000:1) and exposure times up to 72 hours was analyzed for Ni NWs of 5.4 μm in length, as well as for Ni ions. The results show that cytotoxicity markedly increases past 24 hours of incubation. Cellular uptake of NWs takes place through the phagocytosis pathway, with a fraction of the dose of NWs dissolved inside the cells. Cell death results from a combination of apoptosis and necrosis, where the latter is the outcome of the secondary necrosis pathway. The cytotoxicity of Ni ions and Ni NWs dissolution studies suggest a synergistic toxicity between NW aspect ratio and dissolved Ni, with the cytotoxic effects markedly increasing after 24 hours of incubation.</p>en
dc.publisherFigshareen
dc.rightsCC-BYen
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/en
dc.subjectBiochemistryen
dc.subjectSpace Scienceen
dc.subjectMedicineen
dc.subjectCell Biologyen
dc.subjectPharmacologyen
dc.subjectBiotechnologyen
dc.subjectImmunologyen
dc.subjectHematologyen
dc.titleSupplementary Material for: Cytotoxicity and intracellular dissolution of nickel nanowiresen
dc.typeDataseten
dc.contributor.departmentBioscience Programen
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.contributor.departmentChemical and Biological Engineering Programen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
kaust.authorContreras, Maria F.en
kaust.authorVidal, Enrique Vilanovaen
kaust.authorFelix Servin, Laura P.en
kaust.authorMargineanu, Michael B.en
kaust.authorRavasi, Timothyen
kaust.authorKosel, Jürgenen
dc.type.resourceCollectionen
dc.relation.isSupplementToCytotoxicity and intracellular dissolution of nickel nanowires 2015:1 Nanotoxicologyen
dc.relation.isSupplementToDOI:10.3109/17435390.2015.1132343en
dc.relation.isSupplementToHANDLE:http://hdl.handle.net/10754/592811en
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