Performance of thermophilic anaerobic digesters using inoculum mixes with enhanced methanogenic diversity

Handle URI:
http://hdl.handle.net/10754/624042
Title:
Performance of thermophilic anaerobic digesters using inoculum mixes with enhanced methanogenic diversity
Authors:
Ghanimeh, Sophia; El-Fadel, Mutasem ( 0000-0003-2379-9006 ) ; Saikaly, Pascal ( 0000-0001-7678-3986 )
Abstract:
BACKGROUND Reportedly, various mixes of seeds were quasi-randomly selected to startup anaerobic digesters. In contrast, this study examines the impact of inoculating thermophilic anaerobic digesters with a designed mix of non-acclimated seeds based on their methanogen composition, using Quantitative Polymerase Chain Reaction (QPCR) of 16S rRNA gene, to achieve high abundance and diversity of methanogens. RESULTS Based on QPCR results, two seed mixes were selected to inoculate two anaerobic digesters: digester (A) was inoculated with a control seed consisting of digestate, manure, and activated sludge; and digester (B) was inoculated with a further methanogen-enriched seed consisting of the control seed with added compost and leachate. Both seed combinations yielded a balanced microflora that is able to achieve a successful startup. However, upon reaching steady state, digester B exhibited lower propionate levels, resulting in lower VFA concentration and increased buffering capacity, indicating greater stability. Acetotrophs and hydrogenotrophs were dominated by Methanosarcinaceae and Methanobacteriales, respectively, in both digesters, exhibiting an average ratio of 66-to-34% in A and 76-to-24% in B during steady state. CONCLUSION The inoculation strategy in digester B resulted in improved stability, lower propionate concentration and 10% higher relative abundance of acetotrophs.
KAUST Department:
Water Desalination and Reuse Research Center (WDRC)
Citation:
Ghanimeh S, El-Fadel M, Saikaly PE (2017) Performance of thermophilic anaerobic digesters using inoculum mixes with enhanced methanogenic diversity. Journal of Chemical Technology & Biotechnology. Available: http://dx.doi.org/10.1002/jctb.5341.
Publisher:
Wiley-Blackwell
Journal:
Journal of Chemical Technology & Biotechnology
Issue Date:
30-May-2017
DOI:
10.1002/jctb.5341
Type:
Article
ISSN:
0268-2575
Sponsors:
This study was supported by the National Council for Scientific Research-Lebanon, and the Masri Institute of Energy and Natural Resources at the American University of Beirut. The experimental setup was obtained through a grant from the US Agency for International Development. Special thanks are extended to Mr. Khaled Salam for his assistance in performing the QPCR analysis.
Additional Links:
http://onlinelibrary.wiley.com/doi/10.1002/jctb.5341/abstract
Appears in Collections:
Articles; Water Desalination and Reuse Research Center (WDRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorGhanimeh, Sophiaen
dc.contributor.authorEl-Fadel, Mutasemen
dc.contributor.authorSaikaly, Pascalen
dc.date.accessioned2017-06-05T06:02:24Z-
dc.date.available2017-06-05T06:02:24Z-
dc.date.issued2017-05-30en
dc.identifier.citationGhanimeh S, El-Fadel M, Saikaly PE (2017) Performance of thermophilic anaerobic digesters using inoculum mixes with enhanced methanogenic diversity. Journal of Chemical Technology & Biotechnology. Available: http://dx.doi.org/10.1002/jctb.5341.en
dc.identifier.issn0268-2575en
dc.identifier.doi10.1002/jctb.5341en
dc.identifier.urihttp://hdl.handle.net/10754/624042-
dc.description.abstractBACKGROUND Reportedly, various mixes of seeds were quasi-randomly selected to startup anaerobic digesters. In contrast, this study examines the impact of inoculating thermophilic anaerobic digesters with a designed mix of non-acclimated seeds based on their methanogen composition, using Quantitative Polymerase Chain Reaction (QPCR) of 16S rRNA gene, to achieve high abundance and diversity of methanogens. RESULTS Based on QPCR results, two seed mixes were selected to inoculate two anaerobic digesters: digester (A) was inoculated with a control seed consisting of digestate, manure, and activated sludge; and digester (B) was inoculated with a further methanogen-enriched seed consisting of the control seed with added compost and leachate. Both seed combinations yielded a balanced microflora that is able to achieve a successful startup. However, upon reaching steady state, digester B exhibited lower propionate levels, resulting in lower VFA concentration and increased buffering capacity, indicating greater stability. Acetotrophs and hydrogenotrophs were dominated by Methanosarcinaceae and Methanobacteriales, respectively, in both digesters, exhibiting an average ratio of 66-to-34% in A and 76-to-24% in B during steady state. CONCLUSION The inoculation strategy in digester B resulted in improved stability, lower propionate concentration and 10% higher relative abundance of acetotrophs.en
dc.description.sponsorshipThis study was supported by the National Council for Scientific Research-Lebanon, and the Masri Institute of Energy and Natural Resources at the American University of Beirut. The experimental setup was obtained through a grant from the US Agency for International Development. Special thanks are extended to Mr. Khaled Salam for his assistance in performing the QPCR analysis.en
dc.publisherWiley-Blackwellen
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1002/jctb.5341/abstracten
dc.rightsThis is the peer reviewed version of the following article: Performance of thermophilic anaerobic digesters using inoculum mixes with enhanced methanogenic diversity, which has been published in final form at http://doi.org/10.1002/jctb.5341. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.en
dc.subjectAnaerobic digestionen
dc.subjectBiomassen
dc.subjectWaste treatment and minimizationen
dc.titlePerformance of thermophilic anaerobic digesters using inoculum mixes with enhanced methanogenic diversityen
dc.typeArticleen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.identifier.journalJournal of Chemical Technology & Biotechnologyen
dc.eprint.versionPost-printen
dc.contributor.institutionDepartment of Civil and Environmental Engineering; American University of Beiruten
dc.contributor.institutionDepartment of Civil and Environmental Engineering; Notre Dame University-Louaizeen
kaust.authorSaikaly, Pascalen
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