RRM domain of Arabidopsis splicing factor SF1 is important for pre-mRNA splicing of a specific set of genes

Handle URI:
http://hdl.handle.net/10754/623904
Title:
RRM domain of Arabidopsis splicing factor SF1 is important for pre-mRNA splicing of a specific set of genes
Authors:
Lee, Keh Chien; Jang, Yun Hee; Kim, SoonKap; Park, Hyo-Young; Thu, May Phyo; Lee, Jeong Hwan; Kim, Jeong-Kook
Abstract:
The RNA recognition motif of Arabidopsis splicing factor SF1 affects the alternative splicing of FLOWERING LOCUS M pre-mRNA and a heat shock transcription factor HsfA2 pre-mRNA. Splicing factor 1 (SF1) plays a crucial role in 3' splice site recognition by binding directly to the intron branch point. Although plant SF1 proteins possess an RNA recognition motif (RRM) domain that is absent in its fungal and metazoan counterparts, the role of the RRM domain in SF1 function has not been characterized. Here, we show that the RRM domain differentially affects the full function of the Arabidopsis thaliana AtSF1 protein under different experimental conditions. For example, the deletion of RRM domain influences AtSF1-mediated control of flowering time, but not the abscisic acid sensitivity response during seed germination. The alternative splicing of FLOWERING LOCUS M (FLM) pre-mRNA is involved in flowering time control. We found that the RRM domain of AtSF1 protein alters the production of alternatively spliced FLM-β transcripts. We also found that the RRM domain affects the alternative splicing of a heat shock transcription factor HsfA2 pre-mRNA, thereby mediating the heat stress response. Taken together, our results suggest the importance of RRM domain for AtSF1-mediated alternative splicing of a subset of genes involved in the regulation of flowering and adaptation to heat stress.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Center for Desert Agriculture
Citation:
Lee KC, Jang YH, Kim S-K, Park H-Y, Thu MP, et al. (2017) RRM domain of Arabidopsis splicing factor SF1 is important for pre-mRNA splicing of a specific set of genes. Plant Cell Reports. Available: http://dx.doi.org/10.1007/s00299-017-2140-1.
Publisher:
Springer Nature
Journal:
Plant Cell Reports
Issue Date:
11-Apr-2017
DOI:
10.1007/s00299-017-2140-1
Type:
Article
ISSN:
0721-7714; 1432-203X
Sponsors:
This work was supported by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (2014R1A1A2055796 to J.-K. Kim) and a Korea University Grant (to J.-K. Kim).
Additional Links:
http://link.springer.com/article/10.1007%2Fs00299-017-2140-1
Appears in Collections:
Articles; Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLee, Keh Chienen
dc.contributor.authorJang, Yun Heeen
dc.contributor.authorKim, SoonKapen
dc.contributor.authorPark, Hyo-Youngen
dc.contributor.authorThu, May Phyoen
dc.contributor.authorLee, Jeong Hwanen
dc.contributor.authorKim, Jeong-Kooken
dc.date.accessioned2017-05-31T11:23:12Z-
dc.date.available2017-05-31T11:23:12Z-
dc.date.issued2017-04-11en
dc.identifier.citationLee KC, Jang YH, Kim S-K, Park H-Y, Thu MP, et al. (2017) RRM domain of Arabidopsis splicing factor SF1 is important for pre-mRNA splicing of a specific set of genes. Plant Cell Reports. Available: http://dx.doi.org/10.1007/s00299-017-2140-1.en
dc.identifier.issn0721-7714en
dc.identifier.issn1432-203Xen
dc.identifier.doi10.1007/s00299-017-2140-1en
dc.identifier.urihttp://hdl.handle.net/10754/623904-
dc.description.abstractThe RNA recognition motif of Arabidopsis splicing factor SF1 affects the alternative splicing of FLOWERING LOCUS M pre-mRNA and a heat shock transcription factor HsfA2 pre-mRNA. Splicing factor 1 (SF1) plays a crucial role in 3' splice site recognition by binding directly to the intron branch point. Although plant SF1 proteins possess an RNA recognition motif (RRM) domain that is absent in its fungal and metazoan counterparts, the role of the RRM domain in SF1 function has not been characterized. Here, we show that the RRM domain differentially affects the full function of the Arabidopsis thaliana AtSF1 protein under different experimental conditions. For example, the deletion of RRM domain influences AtSF1-mediated control of flowering time, but not the abscisic acid sensitivity response during seed germination. The alternative splicing of FLOWERING LOCUS M (FLM) pre-mRNA is involved in flowering time control. We found that the RRM domain of AtSF1 protein alters the production of alternatively spliced FLM-β transcripts. We also found that the RRM domain affects the alternative splicing of a heat shock transcription factor HsfA2 pre-mRNA, thereby mediating the heat stress response. Taken together, our results suggest the importance of RRM domain for AtSF1-mediated alternative splicing of a subset of genes involved in the regulation of flowering and adaptation to heat stress.en
dc.description.sponsorshipThis work was supported by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (2014R1A1A2055796 to J.-K. Kim) and a Korea University Grant (to J.-K. Kim).en
dc.publisherSpringer Natureen
dc.relation.urlhttp://link.springer.com/article/10.1007%2Fs00299-017-2140-1en
dc.subjectHeat stressen
dc.subjectAlternative splicingen
dc.subjectFlowering timeen
dc.subjectRrm Domainen
dc.subjectFlmen
dc.subjectAtsf1en
dc.titleRRM domain of Arabidopsis splicing factor SF1 is important for pre-mRNA splicing of a specific set of genesen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalPlant Cell Reportsen
dc.contributor.institutionDivision of Life Sciences, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea.en
dc.contributor.institutionDepartment of Life Sciences, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju, Jeollabuk-do, 54896, Republic of Korea. jhwanlee90@jbnu.ac.kr.en
dc.contributor.institutionDivision of Life Sciences, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea. jkkim@korea.ac.kr.en
kaust.authorKim, SoonKapen
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