Prevalent and persistent viral infection in cultures of the coral algal endosymbiont Symbiodinium

Handle URI:
http://hdl.handle.net/10754/623895
Title:
Prevalent and persistent viral infection in cultures of the coral algal endosymbiont Symbiodinium
Authors:
Weynberg, Karen D.; Neave, Matthew J.; Clode, Peta L.; Voolstra, Christian R. ( 0000-0003-4555-3795 ) ; Brownlee, Christopher; Laffy, Patrick; Webster, Nicole S.; Levin, Rachel A.; Wood-Charlson, Elisha M.; Oppen, Madeleine J. H.
Abstract:
Reef corals are under threat from bleaching and disease outbreaks that target both the host animal and the algal symbionts within the coral holobiont. A viral origin for coral bleaching has been hypothesized, but direct evidence has remained elusive. Using a multifaceted approach incorporating flow cytometry, transmission electron microscopy, DNA and RNA virome sequencing, we show that type C1 Symbiodinium cultures host a nucleocytoplasmic large double-stranded DNA virus (NCLDV) related to Phycodnaviridae and Mimiviridae, a novel filamentous virus of unknown phylogenetic affiliation, and a single-stranded RNA virus related to retroviruses. We discuss implications of these findings for laboratory-based experiments using Symbiodinium cultures.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Red Sea Research Center (RSRC)
Citation:
Weynberg KD, Neave M, Clode PL, Voolstra CR, Brownlee C, et al. (2017) Prevalent and persistent viral infection in cultures of the coral algal endosymbiont Symbiodinium . Coral Reefs. Available: http://dx.doi.org/10.1007/s00338-017-1568-7.
Publisher:
Springer Nature
Journal:
Coral Reefs
Issue Date:
17-Mar-2017
DOI:
10.1007/s00338-017-1568-7
Type:
Article
ISSN:
0722-4028; 1432-0975
Sponsors:
We acknowledge the technical support from Victor Beltran at the AIMS Symbiont Culture Facility and the scientific and technical assistance of the Australian Microscopy and Microanalysis Research Facility (AMMRF) at the Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, a facility funded by the University and the Western Australian and Australian governments. We acknowledge funding from the Australian Research Council SuperScience Fellowship #FS110200034 to K.D.W. and Future Fellowships #FT100100088 and #FT120100480 to M.v.O and N.S.W., respectively. Research reported in this publication was supported by King Abdullah University of Science and Technology.
Additional Links:
http://link.springer.com/article/10.1007/s00338-017-1568-7
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorWeynberg, Karen D.en
dc.contributor.authorNeave, Matthew J.en
dc.contributor.authorClode, Peta L.en
dc.contributor.authorVoolstra, Christian R.en
dc.contributor.authorBrownlee, Christopheren
dc.contributor.authorLaffy, Patricken
dc.contributor.authorWebster, Nicole S.en
dc.contributor.authorLevin, Rachel A.en
dc.contributor.authorWood-Charlson, Elisha M.en
dc.contributor.authorOppen, Madeleine J. H.en
dc.date.accessioned2017-05-31T11:23:12Z-
dc.date.available2017-05-31T11:23:12Z-
dc.date.issued2017-03-17en
dc.identifier.citationWeynberg KD, Neave M, Clode PL, Voolstra CR, Brownlee C, et al. (2017) Prevalent and persistent viral infection in cultures of the coral algal endosymbiont Symbiodinium . Coral Reefs. Available: http://dx.doi.org/10.1007/s00338-017-1568-7.en
dc.identifier.issn0722-4028en
dc.identifier.issn1432-0975en
dc.identifier.doi10.1007/s00338-017-1568-7en
dc.identifier.urihttp://hdl.handle.net/10754/623895-
dc.description.abstractReef corals are under threat from bleaching and disease outbreaks that target both the host animal and the algal symbionts within the coral holobiont. A viral origin for coral bleaching has been hypothesized, but direct evidence has remained elusive. Using a multifaceted approach incorporating flow cytometry, transmission electron microscopy, DNA and RNA virome sequencing, we show that type C1 Symbiodinium cultures host a nucleocytoplasmic large double-stranded DNA virus (NCLDV) related to Phycodnaviridae and Mimiviridae, a novel filamentous virus of unknown phylogenetic affiliation, and a single-stranded RNA virus related to retroviruses. We discuss implications of these findings for laboratory-based experiments using Symbiodinium cultures.en
dc.description.sponsorshipWe acknowledge the technical support from Victor Beltran at the AIMS Symbiont Culture Facility and the scientific and technical assistance of the Australian Microscopy and Microanalysis Research Facility (AMMRF) at the Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, a facility funded by the University and the Western Australian and Australian governments. We acknowledge funding from the Australian Research Council SuperScience Fellowship #FS110200034 to K.D.W. and Future Fellowships #FT100100088 and #FT120100480 to M.v.O and N.S.W., respectively. Research reported in this publication was supported by King Abdullah University of Science and Technology.en
dc.publisherSpringer Natureen
dc.relation.urlhttp://link.springer.com/article/10.1007/s00338-017-1568-7en
dc.subjectVirusesen
dc.subjectSymbiodiniumen
dc.subjectSymbiosisen
dc.subjectDinoflagellate culturesen
dc.subjectCoral bleachingen
dc.subjectScleractiniaen
dc.titlePrevalent and persistent viral infection in cultures of the coral algal endosymbiont Symbiodiniumen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalCoral Reefsen
dc.contributor.institutionAustralian Institute of Marine Science, Townsville, Australiaen
dc.contributor.institutionCentre for Microscopy, Characterisation and Analysis, The University of Western Australia, Perth, Australiaen
dc.contributor.institutionThe Oceans Institute, The University of Western Australia, Perth, Australiaen
dc.contributor.institutionBiological Resources Imaging Laboratory, University of New South Wales, Sydney, Australiaen
dc.contributor.institutionCentre for Marine Bio-Innovation, The University of New South Wales, Sydney, Australiaen
dc.contributor.institutionPlant Functional Biology and Climate Change Cluster, University of Technology Sydney, Sydney, Australiaen
dc.contributor.institutionCenter for Microbial Oceanography: Research and Education, University of Hawai‘i at Mānoa, Honolulu, USAen
dc.contributor.institutionSchool of BioSciences, The University of Melbourne, Parkville, Melbourne, Australiaen
kaust.authorNeave, Matthew J.en
kaust.authorVoolstra, Christian R.en
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