Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expression

Handle URI:
http://hdl.handle.net/10754/623891
Title:
Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expression
Authors:
Nagymihály, Marianna; Veluchamy, Alaguraj; Györgypál, Zoltán; Ariel, Federico; Jégu, Teddy; Benhamed, Moussa; Szűcs, Attila; Kereszt, Attila ( 0000-0002-4421-6554 ) ; Mergaert, Peter; Kondorosi, Éva
Abstract:
The formation of symbiotic nodule cells in Medicago truncatula is driven by successive endoreduplication cycles and transcriptional reprogramming in different temporal waves including the activation of more than 600 cysteine-rich NCR genes expressed only in nodules. We show here that the transcriptional waves correlate with growing ploidy levels and have investigated how the epigenome changes during endoreduplication cycles. Differential DNA methylation was found in only a small subset of symbiotic nodule-specific genes, including more than half of the NCR genes, whereas in most genes DNA methylation was unaffected by the ploidy levels and was independent of the genes' active or repressed state. On the other hand, expression of nodule-specific genes correlated with ploidy-dependent opening of the chromatin as well as, in a subset of tested genes, with reduced H3K27me3 levels combined with enhanced H3K9ac levels. Our results suggest that endoreduplication-dependent epigenetic changes contribute to transcriptional reprogramming in the differentiation of symbiotic cells.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Nagymihály M, Veluchamy A, Györgypál Z, Ariel F, Jégu T, et al. (2017) Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expression. Proceedings of the National Academy of Sciences 114: 4543–4548. Available: http://dx.doi.org/10.1073/pnas.1704211114.
Publisher:
Proceedings of the National Academy of Sciences
Journal:
Proceedings of the National Academy of Sciences
Issue Date:
13-Apr-2017
DOI:
10.1073/pnas.1704211114
Type:
Article
ISSN:
0027-8424; 1091-6490
Sponsors:
We thank Célia Casset for help with the qPCR experiments, Dr. Mickael Bourge for the flow cytometry sorting of nodule nuclei, and Dr. István Nagy for providing the MBD2 protein for the MeDIP experiment. This work benefitted from the facilities and expertise of the Imagif Cell Biology Unit of the Gif campus (which is supported by the Infrastructures en Biologie Santé et Agronomie), the Agence Nationale de la Recherche (ANR) under Investments for the Future programs France-BioImaging Infrastructure Grant ANR-10-INSB-04-01 and Saclay Plant Sciences Grant ANR-10-LABX-0040-SPS, and the Conseil Général de l’Essonne. This work was supported by ANR Grant ANR-13-BSV7-0013 and by European Research Council SYM-BIOTICS Advanced Grant 269067 (to É.K.). M.N. was the recipient of a PhD grant from CAMPUS France and was supported by the SYM-BIOTICS grant.
Additional Links:
http://www.pnas.org/content/early/2017/04/11/1704211114.full
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorNagymihály, Mariannaen
dc.contributor.authorVeluchamy, Alagurajen
dc.contributor.authorGyörgypál, Zoltánen
dc.contributor.authorAriel, Federicoen
dc.contributor.authorJégu, Teddyen
dc.contributor.authorBenhamed, Moussaen
dc.contributor.authorSzűcs, Attilaen
dc.contributor.authorKereszt, Attilaen
dc.contributor.authorMergaert, Peteren
dc.contributor.authorKondorosi, Évaen
dc.date.accessioned2017-05-31T11:23:12Z-
dc.date.available2017-05-31T11:23:12Z-
dc.date.issued2017-04-13en
dc.identifier.citationNagymihály M, Veluchamy A, Györgypál Z, Ariel F, Jégu T, et al. (2017) Ploidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expression. Proceedings of the National Academy of Sciences 114: 4543–4548. Available: http://dx.doi.org/10.1073/pnas.1704211114.en
dc.identifier.issn0027-8424en
dc.identifier.issn1091-6490en
dc.identifier.doi10.1073/pnas.1704211114en
dc.identifier.urihttp://hdl.handle.net/10754/623891-
dc.description.abstractThe formation of symbiotic nodule cells in Medicago truncatula is driven by successive endoreduplication cycles and transcriptional reprogramming in different temporal waves including the activation of more than 600 cysteine-rich NCR genes expressed only in nodules. We show here that the transcriptional waves correlate with growing ploidy levels and have investigated how the epigenome changes during endoreduplication cycles. Differential DNA methylation was found in only a small subset of symbiotic nodule-specific genes, including more than half of the NCR genes, whereas in most genes DNA methylation was unaffected by the ploidy levels and was independent of the genes' active or repressed state. On the other hand, expression of nodule-specific genes correlated with ploidy-dependent opening of the chromatin as well as, in a subset of tested genes, with reduced H3K27me3 levels combined with enhanced H3K9ac levels. Our results suggest that endoreduplication-dependent epigenetic changes contribute to transcriptional reprogramming in the differentiation of symbiotic cells.en
dc.description.sponsorshipWe thank Célia Casset for help with the qPCR experiments, Dr. Mickael Bourge for the flow cytometry sorting of nodule nuclei, and Dr. István Nagy for providing the MBD2 protein for the MeDIP experiment. This work benefitted from the facilities and expertise of the Imagif Cell Biology Unit of the Gif campus (which is supported by the Infrastructures en Biologie Santé et Agronomie), the Agence Nationale de la Recherche (ANR) under Investments for the Future programs France-BioImaging Infrastructure Grant ANR-10-INSB-04-01 and Saclay Plant Sciences Grant ANR-10-LABX-0040-SPS, and the Conseil Général de l’Essonne. This work was supported by ANR Grant ANR-13-BSV7-0013 and by European Research Council SYM-BIOTICS Advanced Grant 269067 (to É.K.). M.N. was the recipient of a PhD grant from CAMPUS France and was supported by the SYM-BIOTICS grant.en
dc.publisherProceedings of the National Academy of Sciencesen
dc.relation.urlhttp://www.pnas.org/content/early/2017/04/11/1704211114.fullen
dc.subjectSymbiosisen
dc.subjectDNA methylationen
dc.subjectChromatin Structureen
dc.subjectNodule-specific Cysteine-rich Peptidesen
dc.subjectMedicago Truncatulaen
dc.titlePloidy-dependent changes in the epigenome of symbiotic cells correlate with specific patterns of gene expressionen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalProceedings of the National Academy of Sciencesen
dc.contributor.institutionInstitute for Integrative Biology of the Cell, UMR9198, CNRS, Université Paris-Sud, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, 91198 Gif-sur-Yvette, France.en
dc.contributor.institutionInstitute for Integrative Biology of the Cell, UMR9198, CNRS, Université Paris-Sud, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, 91198 Gif-sur-Yvette, Franceen
dc.contributor.institutionInstitute of Biophysics, Biological Research Centre, Hungarian Academy of Sciences, H-6726 Szeged, Hungary.en
dc.contributor.institutionInstituto de Agrobiotecnología del Litoral, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Consejo Nacional de Investigaciones Científicas y Técnicas, 3000 Santa Fe, Argentina.en
dc.contributor.institutionInstitute of Plant Sciences Paris-Saclay, CNRS, French National Institute for Agricultural Research, Université Paris-Sud, Université Evry, Université Paris-Diderot, Sorbonne Paris-Cité, Universite Paris-Saclay, 91405, Orsay, France.en
dc.contributor.institutionInstitute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, H-6726 Szeged, Hungary.en
dc.contributor.institutionkondorosi.eva@brc.mta.hu peter.mergaert@i2bc.paris-saclay.fr.en
kaust.authorVeluchamy, Alagurajen
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