Imaging and structural studies of DNA–protein complexes and membrane ion channels

Handle URI:
http://hdl.handle.net/10754/623846
Title:
Imaging and structural studies of DNA–protein complexes and membrane ion channels
Authors:
Marini, Monica ( 0000-0001-8182-5239 ) ; Limongi, Tania; Falqui, Andrea ( 0000-0002-1476-7742 ) ; Genovese, Alessandro; Allione, Marco ( 0000-0003-0757-9791 ) ; Moretti, Manola; Lopatin, Sergei; Tirinato, Luca ( 0000-0001-9826-2129 ) ; Das, Gobind ( 0000-0003-0942-681X ) ; Torre, Bruno; Giugni, Andrea; Cesca, F.; Benfenati, F.; Di Fabrizio, Enzo M. ( 0000-0001-5886-4678 )
Abstract:
In bio-imaging by electron microscopy, damage of the sample and limited contrast are the two main hurdles for reaching high image quality. We extend a new preparation method based on nanofabrication and super-hydrophobicity to the imaging and structural studies of nucleic acids, nucleic acid-protein complexes (DNA/Rad51 repair protein complex) and neuronal ion channels (gap-junction, K+ and GABA(A) channels) as paradigms of biological significance and increasing complexity. The preparation method is based on the liquid phase and is compatible with physiological conditions. Only in the very last stage, samples are dried for TEM analysis. Conventional TEM and high-resolution TEM (HRTEM) were used to achieve a resolution of 3.3 and 1.5 angstrom, respectively. The EM dataset quality allows the determination of relevant structural and metrological information on the DNA structure, DNA-protein interactions and ion channels, allowing the identification of specific macromolecules and their structure.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Physical Sciences and Engineering (PSE) Division; Imaging and Characterization Core Lab
Citation:
Marini M, Limongi T, Falqui A, Genovese A, Allione M, et al. (2017) Imaging and structural studies of DNA–protein complexes and membrane ion channels. Nanoscale 9: 2768–2777. Available: http://dx.doi.org/10.1039/c6nr07958j.
Publisher:
Royal Society of Chemistry (RSC)
Journal:
Nanoscale
Issue Date:
17-Jan-2017
DOI:
10.1039/c6nr07958j
Type:
Article
ISSN:
2040-3364; 2040-3372
Sponsors:
The authors acknowledge financial support from the KAUST start-up funding and from the Italian Ministry of Health under project no. GR-2010-2320665 and GR-2010-2311677.
Additional Links:
http://pubs.rsc.org/en/Content/ArticleLanding/2017/NR/C6NR07958J#!divAbstract
Appears in Collections:
Articles; Advanced Nanofabrication, Imaging and Characterization Core Lab; Physical Sciences and Engineering (PSE) Division; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorMarini, Monicaen
dc.contributor.authorLimongi, Taniaen
dc.contributor.authorFalqui, Andreaen
dc.contributor.authorGenovese, Alessandroen
dc.contributor.authorAllione, Marcoen
dc.contributor.authorMoretti, Manolaen
dc.contributor.authorLopatin, Sergeien
dc.contributor.authorTirinato, Lucaen
dc.contributor.authorDas, Gobinden
dc.contributor.authorTorre, Brunoen
dc.contributor.authorGiugni, Andreaen
dc.contributor.authorCesca, F.en
dc.contributor.authorBenfenati, F.en
dc.contributor.authorDi Fabrizio, Enzo M.en
dc.date.accessioned2017-05-31T11:23:09Z-
dc.date.available2017-05-31T11:23:09Z-
dc.date.issued2017-01-17en
dc.identifier.citationMarini M, Limongi T, Falqui A, Genovese A, Allione M, et al. (2017) Imaging and structural studies of DNA–protein complexes and membrane ion channels. Nanoscale 9: 2768–2777. Available: http://dx.doi.org/10.1039/c6nr07958j.en
dc.identifier.issn2040-3364en
dc.identifier.issn2040-3372en
dc.identifier.doi10.1039/c6nr07958jen
dc.identifier.urihttp://hdl.handle.net/10754/623846-
dc.description.abstractIn bio-imaging by electron microscopy, damage of the sample and limited contrast are the two main hurdles for reaching high image quality. We extend a new preparation method based on nanofabrication and super-hydrophobicity to the imaging and structural studies of nucleic acids, nucleic acid-protein complexes (DNA/Rad51 repair protein complex) and neuronal ion channels (gap-junction, K+ and GABA(A) channels) as paradigms of biological significance and increasing complexity. The preparation method is based on the liquid phase and is compatible with physiological conditions. Only in the very last stage, samples are dried for TEM analysis. Conventional TEM and high-resolution TEM (HRTEM) were used to achieve a resolution of 3.3 and 1.5 angstrom, respectively. The EM dataset quality allows the determination of relevant structural and metrological information on the DNA structure, DNA-protein interactions and ion channels, allowing the identification of specific macromolecules and their structure.en
dc.description.sponsorshipThe authors acknowledge financial support from the KAUST start-up funding and from the Italian Ministry of Health under project no. GR-2010-2320665 and GR-2010-2311677.en
dc.publisherRoyal Society of Chemistry (RSC)en
dc.relation.urlhttp://pubs.rsc.org/en/Content/ArticleLanding/2017/NR/C6NR07958J#!divAbstracten
dc.titleImaging and structural studies of DNA–protein complexes and membrane ion channelsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentImaging and Characterization Core Laben
dc.identifier.journalNanoscaleen
dc.contributor.institutionCenter for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia, Genova, , Italyen
kaust.authorMarini, Monicaen
kaust.authorLimongi, Taniaen
kaust.authorFalqui, Andreaen
kaust.authorGenovese, Alessandroen
kaust.authorAllione, Marcoen
kaust.authorMoretti, Manolaen
kaust.authorLopatin, Sergeien
kaust.authorTirinato, Lucaen
kaust.authorDas, Gobinden
kaust.authorTorre, Brunoen
kaust.authorGiugni, Andreaen
kaust.authorDi Fabrizio, Enzo M.en
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.