Constitutively active Arabidopsis MAP Kinase 3 triggers defense responses involving salicylic acid and SUMM2 resistance protein

Handle URI:
http://hdl.handle.net/10754/623810
Title:
Constitutively active Arabidopsis MAP Kinase 3 triggers defense responses involving salicylic acid and SUMM2 resistance protein
Authors:
Genot, Baptiste; Lang, Julien; Berriri, Souha; Garmier, Marie; Gilard, Françoise; Pateyron, Stéphanie; Haustraete, Katrien; Van Der Straeten, Dominique; Hirt, Heribert ( 0000-0003-3119-9633 ) ; Colcombet, Jean
Abstract:
Mitogen-activated protein kinases (MAPKs) are important regulators of plant immunity. Most of the knowledge about the function of these pathways is derived from loss-of-function approaches. Using a gain-of-function approach, we investigated the responses controlled by a constitutively active (CA) MPK3 in Arabidopsis thaliana. CA-MPK3 plants are dwarfed and display a massive de-repression of defense genes associated with spontaneous cell death as well as accumulation of reactive oxygen species (ROS), phytoalexins and the stress-related hormones ethylene and salicylic acid (SA). Remarkably CA-MPK3/sid2 and CA-MPK3/ein2-50 lines which are impaired in SA synthesis and ethylene signaling, respectively, retain most of the CA-MPK3-associated phenotypes, indicating that constitutive activity of MPK3 can bypass SA and ethylene signaling to activate defense responses. A comparative analysis of the molecular phenotypes of CA-MPK3 and mpk4 autoimmunity suggested convergence between the MPK3 and MPK4-guarding modules. In support of this model, CA-MPK3 crosses with summ1 and summ2, two known suppressors of mpk4, resulted in a partial reversion of the CA-MPK3 phenotypes. Overall, our data unravel a novel mechanism by which the MAPK signaling network contributes to a robust defense response system.
KAUST Department:
KAUST CITY: Thuwal POSTAL_CODE: 23955-6900 Saudi Arabia [SA].
Citation:
Genot B, Lang J, Berriri S, Garmier M, Gilard F, et al. (2017) Constitutively active Arabidopsis MAP Kinase 3 triggers defense responses involving salicylic acid and SUMM2 resistance protein. Plant Physiology: pp.00378.2017. Available: http://dx.doi.org/10.1104/pp.17.00378.
Publisher:
American Society of Plant Biologists (ASPB)
Journal:
Plant Physiology
Issue Date:
12-Apr-2017
DOI:
10.1104/pp.17.00378
Type:
Article
ISSN:
0032-0889; 1532-2548
Sponsors:
We thank Yuelin Zhang and Jane Parker for summ1/2 and sid2 mutants respectively, Filip Vandenbussche for ethylene measurement and Jean Bigeard for helpful discussions and his critical reading of the manuscript. This work was largely supported by the Institut National de Recherche Agronomique (INRA),the Agence Nationale de la echerche (ANR) and a grant (LabEx Saclay Plant Sciences-SPS, reference ANR-10-LABX-0040-SPS), managed by the French National Research Agency under an Investments for the Future program (reference n°ANR-11-IDEX-0003-02). B.G. was supported by French Ministry of Research PhD fellowship. D.V.D.S. gratefully acknowledges the Research Foundation Flanders (FWO G.0656.13N) and Ghent University (Bijzonder Onderzoeksfonds, BOF-BAS) for financial support.
Additional Links:
http://www.plantphysiol.org/content/early/2017/04/11/pp.17.00378.1
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorGenot, Baptisteen
dc.contributor.authorLang, Julienen
dc.contributor.authorBerriri, Souhaen
dc.contributor.authorGarmier, Marieen
dc.contributor.authorGilard, Françoiseen
dc.contributor.authorPateyron, Stéphanieen
dc.contributor.authorHaustraete, Katrienen
dc.contributor.authorVan Der Straeten, Dominiqueen
dc.contributor.authorHirt, Heriberten
dc.contributor.authorColcombet, Jeanen
dc.date.accessioned2017-05-31T11:23:06Z-
dc.date.available2017-05-31T11:23:06Z-
dc.date.issued2017-04-12en
dc.identifier.citationGenot B, Lang J, Berriri S, Garmier M, Gilard F, et al. (2017) Constitutively active Arabidopsis MAP Kinase 3 triggers defense responses involving salicylic acid and SUMM2 resistance protein. Plant Physiology: pp.00378.2017. Available: http://dx.doi.org/10.1104/pp.17.00378.en
dc.identifier.issn0032-0889en
dc.identifier.issn1532-2548en
dc.identifier.doi10.1104/pp.17.00378en
dc.identifier.urihttp://hdl.handle.net/10754/623810-
dc.description.abstractMitogen-activated protein kinases (MAPKs) are important regulators of plant immunity. Most of the knowledge about the function of these pathways is derived from loss-of-function approaches. Using a gain-of-function approach, we investigated the responses controlled by a constitutively active (CA) MPK3 in Arabidopsis thaliana. CA-MPK3 plants are dwarfed and display a massive de-repression of defense genes associated with spontaneous cell death as well as accumulation of reactive oxygen species (ROS), phytoalexins and the stress-related hormones ethylene and salicylic acid (SA). Remarkably CA-MPK3/sid2 and CA-MPK3/ein2-50 lines which are impaired in SA synthesis and ethylene signaling, respectively, retain most of the CA-MPK3-associated phenotypes, indicating that constitutive activity of MPK3 can bypass SA and ethylene signaling to activate defense responses. A comparative analysis of the molecular phenotypes of CA-MPK3 and mpk4 autoimmunity suggested convergence between the MPK3 and MPK4-guarding modules. In support of this model, CA-MPK3 crosses with summ1 and summ2, two known suppressors of mpk4, resulted in a partial reversion of the CA-MPK3 phenotypes. Overall, our data unravel a novel mechanism by which the MAPK signaling network contributes to a robust defense response system.en
dc.description.sponsorshipWe thank Yuelin Zhang and Jane Parker for summ1/2 and sid2 mutants respectively, Filip Vandenbussche for ethylene measurement and Jean Bigeard for helpful discussions and his critical reading of the manuscript. This work was largely supported by the Institut National de Recherche Agronomique (INRA),the Agence Nationale de la echerche (ANR) and a grant (LabEx Saclay Plant Sciences-SPS, reference ANR-10-LABX-0040-SPS), managed by the French National Research Agency under an Investments for the Future program (reference n°ANR-11-IDEX-0003-02). B.G. was supported by French Ministry of Research PhD fellowship. D.V.D.S. gratefully acknowledges the Research Foundation Flanders (FWO G.0656.13N) and Ghent University (Bijzonder Onderzoeksfonds, BOF-BAS) for financial support.en
dc.publisherAmerican Society of Plant Biologists (ASPB)en
dc.relation.urlhttp://www.plantphysiol.org/content/early/2017/04/11/pp.17.00378.1en
dc.titleConstitutively active Arabidopsis MAP Kinase 3 triggers defense responses involving salicylic acid and SUMM2 resistance proteinen
dc.typeArticleen
dc.contributor.departmentKAUST CITY: Thuwal POSTAL_CODE: 23955-6900 Saudi Arabia [SA].en
dc.identifier.journalPlant Physiologyen
dc.contributor.institutionInstitut Jacques Monot CITY: Paris France [FR].en
dc.contributor.institutionInstitute of Plant Sciences - Paris-Saclay (IPS2) CITY: Orsay France [FR].en
dc.contributor.institutionJohn Innes Centre CITY: Norwich Great Britain.en
dc.contributor.institutionUniversité Paris-Sud CITY: Orsay France [FR].en
dc.contributor.institutionIBP-Univ Paris Sud CITY: Orsay France [FR].en
dc.contributor.institutionTranscriptomic Platform, Unité de Recherche en Génomique Végétale (URGV), UMR INRA 1165 - Université d'Evry Val d'Essonne - ERL CNRS 8196 CITY: Evry France [FR].en
dc.contributor.institutionGhent University CITY: Gent Belgium [BE].en
dc.contributor.institutionGhent University CITY: Gent POSTAL_CODE: B-9000 Belgium [BE].en
dc.contributor.institutionInstitute of Plant Sciences - Paris-Saclay (IPS2) CITY: Orsay POSTAL_CODE: 91405 France [FR] jean.colcombet@ips2.universite-paris-saclay.fr.en
kaust.authorHirt, Heriberten
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.