Analyzing Lysosome-Related Organelles by Electron Microscopy

Handle URI:
http://hdl.handle.net/10754/623792
Title:
Analyzing Lysosome-Related Organelles by Electron Microscopy
Authors:
Hurbain, Ilse; Romao, Maryse; Bergam, Ptissam; Heiligenstein, Xavier; Raposo, Graça
Abstract:
Intracellular organelles have a particular morphological signature that can only be appreciated by ultrastructural analysis at the electron microscopy level. Optical imaging and associated methodologies allow to explore organelle localization and their dynamics at the cellular level. Deciphering the biogenesis and functions of lysosomes and lysosome-related organelles (LROs) and their dysfunctions requires their visualization and detailed characterization at high resolution by electron microscopy. Here, we provide detailed protocols for studying LROs by transmission electron microscopy. While conventional electron microscopy and its recent improvements is the method of choice to investigate organelle morphology, immunoelectron microscopy allows to localize organelle components and description of their molecular make up qualitatively and quantitatively.
KAUST Department:
Characterization Core Lab, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia
Citation:
Hurbain I, Romao M, Bergam P, Heiligenstein X, Raposo G (2017) Analyzing Lysosome-Related Organelles by Electron Microscopy. Lysosomes: 43–71. Available: http://dx.doi.org/10.1007/978-1-4939-6934-0_4.
Publisher:
Springer New York
Journal:
Methods in Molecular Biology
Issue Date:
29-Apr-2017
DOI:
10.1007/978-1-4939-6934-0_4
Type:
Book Chapter
ISSN:
1064-3745; 1940-6029
Sponsors:
We are grateful to Institut Curie and the BioImaging platform (PICT IBiSA), member of the France-BioImaging national research infrastructure (ANR-10-INSB-04) and CNRS. Research in our group is supported by the Fondation pour la Recherche Médical (Equipes FRM), Association de Recherche pour le Cancer (ARC), Indian French cooperation (CEFIPRA), Clarins and L’Oréal.
Additional Links:
http://link.springer.com/protocol/10.1007%2F978-1-4939-6934-0_4
Appears in Collections:
Book Chapters

Full metadata record

DC FieldValue Language
dc.contributor.authorHurbain, Ilseen
dc.contributor.authorRomao, Maryseen
dc.contributor.authorBergam, Ptissamen
dc.contributor.authorHeiligenstein, Xavieren
dc.contributor.authorRaposo, Graçaen
dc.date.accessioned2017-05-31T11:23:05Z-
dc.date.available2017-05-31T11:23:05Z-
dc.date.issued2017-04-29en
dc.identifier.citationHurbain I, Romao M, Bergam P, Heiligenstein X, Raposo G (2017) Analyzing Lysosome-Related Organelles by Electron Microscopy. Lysosomes: 43–71. Available: http://dx.doi.org/10.1007/978-1-4939-6934-0_4.en
dc.identifier.issn1064-3745en
dc.identifier.issn1940-6029en
dc.identifier.doi10.1007/978-1-4939-6934-0_4en
dc.identifier.urihttp://hdl.handle.net/10754/623792-
dc.description.abstractIntracellular organelles have a particular morphological signature that can only be appreciated by ultrastructural analysis at the electron microscopy level. Optical imaging and associated methodologies allow to explore organelle localization and their dynamics at the cellular level. Deciphering the biogenesis and functions of lysosomes and lysosome-related organelles (LROs) and their dysfunctions requires their visualization and detailed characterization at high resolution by electron microscopy. Here, we provide detailed protocols for studying LROs by transmission electron microscopy. While conventional electron microscopy and its recent improvements is the method of choice to investigate organelle morphology, immunoelectron microscopy allows to localize organelle components and description of their molecular make up qualitatively and quantitatively.en
dc.description.sponsorshipWe are grateful to Institut Curie and the BioImaging platform (PICT IBiSA), member of the France-BioImaging national research infrastructure (ANR-10-INSB-04) and CNRS. Research in our group is supported by the Fondation pour la Recherche Médical (Equipes FRM), Association de Recherche pour le Cancer (ARC), Indian French cooperation (CEFIPRA), Clarins and L’Oréal.en
dc.publisherSpringer New Yorken
dc.relation.urlhttp://link.springer.com/protocol/10.1007%2F978-1-4939-6934-0_4en
dc.subjectLysosome-related organellesen
dc.subjectTransmission electron microscopyen
dc.subjectChemical fixationen
dc.subjectHigh pressure freezingen
dc.subjectFreeze substitutionen
dc.subjectImmunolabelingen
dc.subjectTokuyasuen
dc.titleAnalyzing Lysosome-Related Organelles by Electron Microscopyen
dc.typeBook Chapteren
dc.contributor.departmentCharacterization Core Lab, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabiaen
dc.identifier.journalMethods in Molecular Biologyen
dc.contributor.institutionInstitut Curie, PSL Research University, CNRS, UMR 144, F-75005, Paris, Franceen
dc.contributor.institutionSorbonne Universités, UPMC Univ Paris 06, CNRS, UMR 144, F-75005, Paris, Franceen
dc.contributor.institutionCell and Tissue Imaging Core Facility PICT-IBiSA, Institut Curie, F-75248, Paris, Franceen
kaust.authorBergam, Ptissamen
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