Molecular and biochemical characterization of the NS1 protein of non-cultured influenza B virus strains circulating in Singapore

Handle URI:
http://hdl.handle.net/10754/623717
Title:
Molecular and biochemical characterization of the NS1 protein of non-cultured influenza B virus strains circulating in Singapore
Authors:
Jumat, Muhammad Raihan; Sugrue, Richard J.; Tan, Boon Huan; Maurer-Stroh, Sebastian; Lee, Raphael Tze Chuen; Wong, Puisan
Abstract:
In this study we compared the NS1 protein of Influenza B/Lee/40 and several non-cultured Influenza B virus clinical strains detected in Singapore. In B/Lee/40 virus-infected cells and in cells expressing the recombinant B/Lee/40 NS1 protein a full-length 35 kDa NS1 protein and a 23 kDa NS1 protein species (p23) were detected. Mutational analysis of the NS1 gene indicated that p23 was generated by a novel cleavage event within the linker domain between an aspartic acid and proline at amino acid residues at positions 92 and 93 respectively (DP92–93), and that p23 contained the first 92 amino acids of the NS1 protein. Sequence analysis of the Singapore strains indicated the presence of either DP92–93 or NP92–93 in the NS1 protein, but protein expression analysis showed that p23 was only detected in NS1 proteins with DP92–93.. An additional adjacent proline residue at position 94 (P94) was present in some strains and correlated with increased p23 levels, suggesting that P94 has a synergistic effect on the cleavage of the NS1 protein. The first 145 amino acids of the NS1 protein are required for inhibition of ISG15-mediated ubiquitination, and our analysis showed that Influenza B viruses circulating in Singapore with DP92–93 expressed truncated NS1 proteins and may differ in their capacity to inhibit ISG15 activity. Thus, DP92–93 in the NS1 protein may confer a disadvantage to Influenza B viruses circulating in the human population and interestingly the low frequency of DP92–93detection in the NS1 protein since 2004 is consistent with this suggestion.
KAUST Department:
Water Desalination and Reuse Research Center (WDRC)
Citation:
Jumat MR, Sugrue RJ, Tan BH, Maurer-Stroh S, Lee RTC, et al. (2016) Molecular and biochemical characterization of the NS1 protein of non-cultured influenza B virus strains circulating in Singapore. Microbial Genomics 2. Available: http://dx.doi.org/10.1099/mgen.0.000082.
Publisher:
Microbiology Society
Journal:
Microbial Genomics
Issue Date:
4-Aug-2016
DOI:
10.1099/mgen.0.000082
Type:
Article
ISSN:
2057-5858; 2057-5858
Sponsors:
Funding for this project and access to clinical specimens were graciously provided by DSO National Laboratories. We thank Thorsten Wolff (Robert Koch Institute) for the NS1 protein antibody. M. R. J. was a recipient of the NTU Post-Graduate Scholarship (Ministry of Education).
Additional Links:
http://mgen.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000082
Appears in Collections:
Articles; Water Desalination and Reuse Research Center (WDRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorJumat, Muhammad Raihanen
dc.contributor.authorSugrue, Richard J.en
dc.contributor.authorTan, Boon Huanen
dc.contributor.authorMaurer-Stroh, Sebastianen
dc.contributor.authorLee, Raphael Tze Chuenen
dc.contributor.authorWong, Puisanen
dc.date.accessioned2017-05-25T10:55:14Z-
dc.date.available2017-05-25T10:55:14Z-
dc.date.issued2016-08-04en
dc.identifier.citationJumat MR, Sugrue RJ, Tan BH, Maurer-Stroh S, Lee RTC, et al. (2016) Molecular and biochemical characterization of the NS1 protein of non-cultured influenza B virus strains circulating in Singapore. Microbial Genomics 2. Available: http://dx.doi.org/10.1099/mgen.0.000082.en
dc.identifier.issn2057-5858en
dc.identifier.issn2057-5858en
dc.identifier.doi10.1099/mgen.0.000082en
dc.identifier.urihttp://hdl.handle.net/10754/623717-
dc.description.abstractIn this study we compared the NS1 protein of Influenza B/Lee/40 and several non-cultured Influenza B virus clinical strains detected in Singapore. In B/Lee/40 virus-infected cells and in cells expressing the recombinant B/Lee/40 NS1 protein a full-length 35 kDa NS1 protein and a 23 kDa NS1 protein species (p23) were detected. Mutational analysis of the NS1 gene indicated that p23 was generated by a novel cleavage event within the linker domain between an aspartic acid and proline at amino acid residues at positions 92 and 93 respectively (DP92–93), and that p23 contained the first 92 amino acids of the NS1 protein. Sequence analysis of the Singapore strains indicated the presence of either DP92–93 or NP92–93 in the NS1 protein, but protein expression analysis showed that p23 was only detected in NS1 proteins with DP92–93.. An additional adjacent proline residue at position 94 (P94) was present in some strains and correlated with increased p23 levels, suggesting that P94 has a synergistic effect on the cleavage of the NS1 protein. The first 145 amino acids of the NS1 protein are required for inhibition of ISG15-mediated ubiquitination, and our analysis showed that Influenza B viruses circulating in Singapore with DP92–93 expressed truncated NS1 proteins and may differ in their capacity to inhibit ISG15 activity. Thus, DP92–93 in the NS1 protein may confer a disadvantage to Influenza B viruses circulating in the human population and interestingly the low frequency of DP92–93detection in the NS1 protein since 2004 is consistent with this suggestion.en
dc.description.sponsorshipFunding for this project and access to clinical specimens were graciously provided by DSO National Laboratories. We thank Thorsten Wolff (Robert Koch Institute) for the NS1 protein antibody. M. R. J. was a recipient of the NTU Post-Graduate Scholarship (Ministry of Education).en
dc.publisherMicrobiology Societyen
dc.relation.urlhttp://mgen.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000082en
dc.rightsThis is an open access article published by the Microbiology Society under the Creative Commons Attribution-NonCommercial Licenseen
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/en
dc.subjectProteolysisen
dc.subjectB/Lee/40en
dc.subjectInfluenza Ben
dc.subjectNS1en
dc.subjectCleavageen
dc.titleMolecular and biochemical characterization of the NS1 protein of non-cultured influenza B virus strains circulating in Singaporeen
dc.typeArticleen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.identifier.journalMicrobial Genomicsen
dc.eprint.versionPost-printen
dc.contributor.institutionSchool of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, 637551, Singaporeen
dc.contributor.institutionDetection and Diagnostics Laboratory, DSO National Laboratories, 27 Medical Drive, 117510, Singaporeen
dc.contributor.institutionBioinformatics Institute (BII) 30 Biopolis Street #07-01, Matrix Building, 138671, Singaporeen
kaust.authorJumat, Muhammad Raihanen
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.