Raman study of lysozyme amyloid fibrils suspended on super-hydrophobic surfaces by shear flow

Handle URI:
http://hdl.handle.net/10754/623703
Title:
Raman study of lysozyme amyloid fibrils suspended on super-hydrophobic surfaces by shear flow
Authors:
Moretti, Manola; Allione, Marco ( 0000-0003-0757-9791 ) ; Marini, Monica ( 0000-0001-8182-5239 ) ; Torre, Bruno; Giugni, Andrea; Limongi, Tania; Das, Gobind ( 0000-0003-0942-681X ) ; Di Fabrizio, Enzo M. ( 0000-0001-5886-4678 )
Abstract:
The shear flow generated at the rim of a drop evaporating on a micro-fabricated super-hydrophobic surface has been used to suspend and orient single/few lysozyme amyloid fibrils between two pillars for substrate-free characterization. Micro Raman spectroscopy performed on extended fibers evidenced a shift of the Amide I band main peak to the value attributed to β-sheet secondary structure, characteristic of the amyloid fibers. In addition, given the orientation sensitivity of the anisotropic molecule, the Raman signal of the main secondary structure was nicely enhanced for a fiber alignment parallel to the polarization direction of the laser. The substrate-free sample generated by this suspending technique is suitable for other structural analysis methods, where fiber crystals are investigated. It could be further employed for generation of arrays and patterns in a controllable fashion, where bio-compatible material is needed.
KAUST Department:
Physical Sciences and Engineering (PSE) Division; Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Moretti M, Allione M, Marini M, Torre B, Giugni A, et al. (2017) Raman study of lysozyme amyloid fibrils suspended on super-hydrophobic surfaces by shear flow. Microelectronic Engineering 178: 194–198. Available: http://dx.doi.org/10.1016/j.mee.2017.05.045.
Publisher:
Elsevier BV
Journal:
Microelectronic Engineering
Issue Date:
19-May-2017
DOI:
10.1016/j.mee.2017.05.045
Type:
Article
ISSN:
0167-9317
Sponsors:
The authors acknowledge financial support from the KAUST start-up funding and from Ministry of Health, Italy under the projects: Project no.: GR-2010-2320665 and Project no.: GR-2010-2311677.
Additional Links:
http://www.sciencedirect.com/science/article/pii/S0167931717302423
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorMoretti, Manolaen
dc.contributor.authorAllione, Marcoen
dc.contributor.authorMarini, Monicaen
dc.contributor.authorTorre, Brunoen
dc.contributor.authorGiugni, Andreaen
dc.contributor.authorLimongi, Taniaen
dc.contributor.authorDas, Gobinden
dc.contributor.authorDi Fabrizio, Enzo M.en
dc.date.accessioned2017-05-23T09:30:36Z-
dc.date.available2017-05-23T09:30:36Z-
dc.date.issued2017-05-19en
dc.identifier.citationMoretti M, Allione M, Marini M, Torre B, Giugni A, et al. (2017) Raman study of lysozyme amyloid fibrils suspended on super-hydrophobic surfaces by shear flow. Microelectronic Engineering 178: 194–198. Available: http://dx.doi.org/10.1016/j.mee.2017.05.045.en
dc.identifier.issn0167-9317en
dc.identifier.doi10.1016/j.mee.2017.05.045en
dc.identifier.urihttp://hdl.handle.net/10754/623703-
dc.description.abstractThe shear flow generated at the rim of a drop evaporating on a micro-fabricated super-hydrophobic surface has been used to suspend and orient single/few lysozyme amyloid fibrils between two pillars for substrate-free characterization. Micro Raman spectroscopy performed on extended fibers evidenced a shift of the Amide I band main peak to the value attributed to β-sheet secondary structure, characteristic of the amyloid fibers. In addition, given the orientation sensitivity of the anisotropic molecule, the Raman signal of the main secondary structure was nicely enhanced for a fiber alignment parallel to the polarization direction of the laser. The substrate-free sample generated by this suspending technique is suitable for other structural analysis methods, where fiber crystals are investigated. It could be further employed for generation of arrays and patterns in a controllable fashion, where bio-compatible material is needed.en
dc.description.sponsorshipThe authors acknowledge financial support from the KAUST start-up funding and from Ministry of Health, Italy under the projects: Project no.: GR-2010-2320665 and Project no.: GR-2010-2311677.en
dc.publisherElsevier BVen
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0167931717302423en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Microelectronic Engineering. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Microelectronic Engineering, [, , (2017-05-19)] DOI: 10.1016/j.mee.2017.05.045 . © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectSuper-hydrophobic micro-patterned surfacesen
dc.subjectRaman spectroscopyen
dc.subjectAmyloid fibrilsen
dc.subjectβ-sheet secondary structureen
dc.titleRaman study of lysozyme amyloid fibrils suspended on super-hydrophobic surfaces by shear flowen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalMicroelectronic Engineeringen
dc.eprint.versionPost-printen
dc.contributor.institutionBio-Nanotechnology and Engineering for Medicine (BIONEM), Department of Experimental and Clinical Medicine, University of Magna Graecia Viale Europa, Germaneto, Catanzaro 88100, Italyen
kaust.authorMoretti, Manolaen
kaust.authorAllione, Marcoen
kaust.authorMarini, Monicaen
kaust.authorTorre, Brunoen
kaust.authorGiugni, Andreaen
kaust.authorLimongi, Taniaen
kaust.authorDas, Gobinden
kaust.authorDi Fabrizio, Enzo M.en
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