Single-cell genomics reveals pyrrolysine-encoding potential in members of uncultivated archaeal candidate division MSBL1

Handle URI:
http://hdl.handle.net/10754/623647
Title:
Single-cell genomics reveals pyrrolysine-encoding potential in members of uncultivated archaeal candidate division MSBL1
Authors:
Guan, Yue ( 0000-0002-5977-6984 ) ; Haroon, Mohamed; Alam, Intikhab; Ferry, James G.; Stingl, Ulrich ( 0000-0002-0684-2597 )
Abstract:
Pyrrolysine (Pyl), the 22nd canonical amino acid, is only decoded and synthesized by a limited number of organisms in the domains Archaea and Bacteria. Pyl is encoded by the amber codon UAG, typically a stop codon. To date, all known Pyl-decoding archaea are able to carry out methylotrophic methanogenesis. The functionality of methylamine methyltransferases, an important component of corrinoid-dependent methyltransfer reactions, depends on the presence of Pyl. Here, we present a putative pyl gene cluster obtained from single-cell genomes of the archaeal Mediterranean Sea Brine Lakes group 1 (MSBL1) from the Red Sea. Functional annotation of the MSBL1 single cell amplified genomes (SAGs) also revealed a complete corrinoid-dependent methyl-transfer pathway suggesting that members of MSBL1 may possibly be capable of synthesizing Pyl and metabolizing methylated amines. This article is protected by copyright. All rights reserved.
KAUST Department:
Red Sea Research Center (RSRC); Computational Bioscience Research Center (CBRC)
Citation:
Guan Y, Haroon MF, Alam I, Ferry JG, Stingl U (2017) Single-cell genomics reveals pyrrolysine-encoding potential in members of uncultivated archaeal candidate division MSBL1. Environmental Microbiology Reports. Available: http://dx.doi.org/10.1111/1758-2229.12545.
Publisher:
Wiley-Blackwell
Journal:
Environmental Microbiology Reports
Issue Date:
11-May-2017
DOI:
10.1111/1758-2229.12545
Type:
Article
ISSN:
1758-2229
Sponsors:
This study was supported by King Abdullah University of Science and Technology (KAUST) through baseline funding and the SEDCO Research Excellence award to US.
Additional Links:
http://onlinelibrary.wiley.com/doi/10.1111/1758-2229.12545/abstract
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Computational Bioscience Research Center (CBRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorGuan, Yueen
dc.contributor.authorHaroon, Mohameden
dc.contributor.authorAlam, Intikhaben
dc.contributor.authorFerry, James G.en
dc.contributor.authorStingl, Ulrichen
dc.date.accessioned2017-05-17T07:41:40Z-
dc.date.available2017-05-17T07:41:40Z-
dc.date.issued2017-05-11en
dc.identifier.citationGuan Y, Haroon MF, Alam I, Ferry JG, Stingl U (2017) Single-cell genomics reveals pyrrolysine-encoding potential in members of uncultivated archaeal candidate division MSBL1. Environmental Microbiology Reports. Available: http://dx.doi.org/10.1111/1758-2229.12545.en
dc.identifier.issn1758-2229en
dc.identifier.doi10.1111/1758-2229.12545en
dc.identifier.urihttp://hdl.handle.net/10754/623647-
dc.description.abstractPyrrolysine (Pyl), the 22nd canonical amino acid, is only decoded and synthesized by a limited number of organisms in the domains Archaea and Bacteria. Pyl is encoded by the amber codon UAG, typically a stop codon. To date, all known Pyl-decoding archaea are able to carry out methylotrophic methanogenesis. The functionality of methylamine methyltransferases, an important component of corrinoid-dependent methyltransfer reactions, depends on the presence of Pyl. Here, we present a putative pyl gene cluster obtained from single-cell genomes of the archaeal Mediterranean Sea Brine Lakes group 1 (MSBL1) from the Red Sea. Functional annotation of the MSBL1 single cell amplified genomes (SAGs) also revealed a complete corrinoid-dependent methyl-transfer pathway suggesting that members of MSBL1 may possibly be capable of synthesizing Pyl and metabolizing methylated amines. This article is protected by copyright. All rights reserved.en
dc.description.sponsorshipThis study was supported by King Abdullah University of Science and Technology (KAUST) through baseline funding and the SEDCO Research Excellence award to US.en
dc.publisherWiley-Blackwellen
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1111/1758-2229.12545/abstracten
dc.rightsThis is the peer reviewed version of the following article: Single-cell genomics reveals pyrrolysine-encoding potential in members of uncultivated archaeal candidate division MSBL1, which has been published in final form at http://doi.org/10.1111/1758-2229.12545. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.en
dc.titleSingle-cell genomics reveals pyrrolysine-encoding potential in members of uncultivated archaeal candidate division MSBL1en
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journalEnvironmental Microbiology Reportsen
dc.eprint.versionPost-printen
dc.contributor.institutionHarvard University, Department of Organismic and Evolutionary Biology; Cambridge MA 02138 USAen
dc.contributor.institutionPennsylvania State University, Department of Biochemistry and Molecular Biology; University Park PA 16802 USAen
dc.contributor.institutionUniversity of Florida/IFAS, Department for Microbiology & Cell Science, Fort Lauderdale Research and Education Center; Davie FL 33314 USAen
kaust.authorGuan, Yueen
kaust.authorHaroon, Mohameden
kaust.authorAlam, Intikhaben
kaust.authorStingl, Ulrichen
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