Viruses-to-mobile genetic elements skew in the deep Atlantis II brine pool sediments

Handle URI:
http://hdl.handle.net/10754/623615
Title:
Viruses-to-mobile genetic elements skew in the deep Atlantis II brine pool sediments
Authors:
Adel, Mustafa; Elbehery, Ali H. A.; Aziz, Sherry K.; Aziz, Ramy K.; Grossart, Hans-Peter; Siam, Rania
Abstract:
The central rift of the Red Sea has 25 brine pools with different physical and geochemical characteristics. Atlantis II (ATIID), Discovery Deeps (DD) and Chain Deep (CD) are characterized by high salinity, temperature and metal content. Several studies reported microbial communities in these brine pools, but few studies addressed the brine pool sediments. Therefore, sediment cores were collected from ATIID, DD, CD brine pools and an adjacent brine-influenced site. Sixteen different lithologic sediment sections were subjected to shotgun DNA pyrosequencing to generate 1.47 billion base pairs (1.47 × 109 bp). We generated sediment-specific reads and attempted to annotate all reads. We report the phylogenetic and biochemical uniqueness of the deepest ATIID sulfur-rich brine pool sediments. In contrary to all other sediment sections, bacteria dominate the deepest ATIID sulfur-rich brine pool sediments. This decrease in virus-to-bacteria ratio in selected sections and depth coincided with an overrepresentation of mobile genetic elements. Skewing in the composition of viruses-to-mobile genetic elements may uniquely contribute to the distinct microbial consortium in sediments in proximity to hydrothermally active vents of the Red Sea and possibly in their surroundings, through differential horizontal gene transfer.
Citation:
Adel M, Elbehery AHA, Aziz SK, Aziz RK, Grossart H-P, et al. (2016) Viruses-to-mobile genetic elements skew in the deep Atlantis II brine pool sediments. Scientific Reports 6. Available: http://dx.doi.org/10.1038/srep32704.
Publisher:
Springer Nature
Journal:
Scientific Reports
KAUST Grant Number:
SA-C0039
Issue Date:
6-Sep-2016
DOI:
10.1038/srep32704
Type:
Article
ISSN:
2045-2322
Sponsors:
This work was initially supported by King Abdullah University for Science and Technology Global Collaborative Partners (GCR) program (Award Number: SA-C0039). The work was funded by an American University in Cairo Faculty (Research) Support Grant to RS. We thank the crew and scientists on board the KAUST Red Sea Expedition in spring 2010, in particular chief scientist Dr. Abdulaziz Al-Suwailem. We acknowledge Professor Hamza El-Dorry, of the American University in Cairo, for help with the initial study design. We also thank Amged Ouf and Dr. Ahmed Abdelaziz of the American University in Cairo for DNA preparation.
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorAdel, Mustafaen
dc.contributor.authorElbehery, Ali H. A.en
dc.contributor.authorAziz, Sherry K.en
dc.contributor.authorAziz, Ramy K.en
dc.contributor.authorGrossart, Hans-Peteren
dc.contributor.authorSiam, Raniaen
dc.date.accessioned2017-05-15T10:35:11Z-
dc.date.available2017-05-15T10:35:11Z-
dc.date.issued2016-09-06en
dc.identifier.citationAdel M, Elbehery AHA, Aziz SK, Aziz RK, Grossart H-P, et al. (2016) Viruses-to-mobile genetic elements skew in the deep Atlantis II brine pool sediments. Scientific Reports 6. Available: http://dx.doi.org/10.1038/srep32704.en
dc.identifier.issn2045-2322en
dc.identifier.doi10.1038/srep32704en
dc.identifier.urihttp://hdl.handle.net/10754/623615-
dc.description.abstractThe central rift of the Red Sea has 25 brine pools with different physical and geochemical characteristics. Atlantis II (ATIID), Discovery Deeps (DD) and Chain Deep (CD) are characterized by high salinity, temperature and metal content. Several studies reported microbial communities in these brine pools, but few studies addressed the brine pool sediments. Therefore, sediment cores were collected from ATIID, DD, CD brine pools and an adjacent brine-influenced site. Sixteen different lithologic sediment sections were subjected to shotgun DNA pyrosequencing to generate 1.47 billion base pairs (1.47 × 109 bp). We generated sediment-specific reads and attempted to annotate all reads. We report the phylogenetic and biochemical uniqueness of the deepest ATIID sulfur-rich brine pool sediments. In contrary to all other sediment sections, bacteria dominate the deepest ATIID sulfur-rich brine pool sediments. This decrease in virus-to-bacteria ratio in selected sections and depth coincided with an overrepresentation of mobile genetic elements. Skewing in the composition of viruses-to-mobile genetic elements may uniquely contribute to the distinct microbial consortium in sediments in proximity to hydrothermally active vents of the Red Sea and possibly in their surroundings, through differential horizontal gene transfer.en
dc.description.sponsorshipThis work was initially supported by King Abdullah University for Science and Technology Global Collaborative Partners (GCR) program (Award Number: SA-C0039). The work was funded by an American University in Cairo Faculty (Research) Support Grant to RS. We thank the crew and scientists on board the KAUST Red Sea Expedition in spring 2010, in particular chief scientist Dr. Abdulaziz Al-Suwailem. We acknowledge Professor Hamza El-Dorry, of the American University in Cairo, for help with the initial study design. We also thank Amged Ouf and Dr. Ahmed Abdelaziz of the American University in Cairo for DNA preparation.en
dc.publisherSpringer Natureen
dc.titleViruses-to-mobile genetic elements skew in the deep Atlantis II brine pool sedimentsen
dc.typeArticleen
dc.identifier.journalScientific Reportsen
dc.contributor.institutionBiology Department, Biotechnology Graduate Program and YJ-Science and Technology Research Center, American University in Cairo, Egypten
dc.contributor.institutionDepartment of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Egypten
dc.contributor.institutionLeibniz-Institute of Freshwater Ecology and Inland Fisheries, Alte Fischerhütte 2, 16775 Stechlin, Germanyen
dc.contributor.institutionInstitute of Biochemistry and Biology, University of Potsdam, Am Neuen Palais 10, 14469 Potsdam, Germanyen
kaust.grant.numberSA-C0039en
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