RNA-Binding Proteins Revisited – The Emerging Arabidopsis mRNA Interactome

Handle URI:
http://hdl.handle.net/10754/623586
Title:
RNA-Binding Proteins Revisited – The Emerging Arabidopsis mRNA Interactome
Authors:
Köster, Tino; Marondedze, Claudius; Meyer, Katja; Staiger, Dorothee
Abstract:
RNA–protein interaction is an important checkpoint to tune gene expression at the RNA level. Global identification of proteins binding in vivo to mRNA has been possible through interactome capture – where proteins are fixed to target RNAs by UV crosslinking and purified through affinity capture of polyadenylated RNA. In Arabidopsis over 500 RNA-binding proteins (RBPs) enriched in UV-crosslinked samples have been identified. As in mammals and yeast, the mRNA interactomes came with a few surprises. For example, a plethora of the proteins caught on RNA had not previously been linked to RNA-mediated processes, for example proteins of intermediary metabolism. Thus, the studies provide unprecedented insights into the composition of the mRNA interactome, highlighting the complexity of RNA-mediated processes.
Citation:
Köster T, Marondedze C, Meyer K, Staiger D (2017) RNA-Binding Proteins Revisited – The Emerging Arabidopsis mRNA Interactome. Trends in Plant Science. Available: http://dx.doi.org/10.1016/j.tplants.2017.03.009.
Publisher:
Elsevier BV
Journal:
Trends in Plant Science
KAUST Grant Number:
CRG3-62140383
Issue Date:
13-Apr-2017
DOI:
10.1016/j.tplants.2017.03.009
Type:
Article
ISSN:
1360-1385
Sponsors:
We thank Prof. Michael Ladomery for comments on the draft of this manuscript. We gratefully acknowledge support by the Deutsche Forschungsgemeinschaft (DFG) through grants STA653 and SPP1530. C.M. was funded by the Office of Competitive Research Grant Program, grant CRG3-62140383, from King Abdullah University of Science and Technology (KAUST). K.M. was funded by the German National Academic Foundation.
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Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorKöster, Tinoen
dc.contributor.authorMarondedze, Claudiusen
dc.contributor.authorMeyer, Katjaen
dc.contributor.authorStaiger, Dorotheeen
dc.date.accessioned2017-05-15T10:35:10Z-
dc.date.available2017-05-15T10:35:10Z-
dc.date.issued2017-04-13en
dc.identifier.citationKöster T, Marondedze C, Meyer K, Staiger D (2017) RNA-Binding Proteins Revisited – The Emerging Arabidopsis mRNA Interactome. Trends in Plant Science. Available: http://dx.doi.org/10.1016/j.tplants.2017.03.009.en
dc.identifier.issn1360-1385en
dc.identifier.doi10.1016/j.tplants.2017.03.009en
dc.identifier.urihttp://hdl.handle.net/10754/623586-
dc.description.abstractRNA–protein interaction is an important checkpoint to tune gene expression at the RNA level. Global identification of proteins binding in vivo to mRNA has been possible through interactome capture – where proteins are fixed to target RNAs by UV crosslinking and purified through affinity capture of polyadenylated RNA. In Arabidopsis over 500 RNA-binding proteins (RBPs) enriched in UV-crosslinked samples have been identified. As in mammals and yeast, the mRNA interactomes came with a few surprises. For example, a plethora of the proteins caught on RNA had not previously been linked to RNA-mediated processes, for example proteins of intermediary metabolism. Thus, the studies provide unprecedented insights into the composition of the mRNA interactome, highlighting the complexity of RNA-mediated processes.en
dc.description.sponsorshipWe thank Prof. Michael Ladomery for comments on the draft of this manuscript. We gratefully acknowledge support by the Deutsche Forschungsgemeinschaft (DFG) through grants STA653 and SPP1530. C.M. was funded by the Office of Competitive Research Grant Program, grant CRG3-62140383, from King Abdullah University of Science and Technology (KAUST). K.M. was funded by the German National Academic Foundation.en
dc.publisherElsevier BVen
dc.subjectRNA-binding proteinen
dc.subjectmRNA interactomeen
dc.subjectUV crosslinkingen
dc.subjectmRNP particlesen
dc.titleRNA-Binding Proteins Revisited – The Emerging Arabidopsis mRNA Interactomeen
dc.typeArticleen
dc.identifier.journalTrends in Plant Scienceen
dc.contributor.institutionMolecular Cell Physiology, Faculty of Biology, Bielefeld University, 33615 Bielefeld, Germanyen
dc.contributor.institutionCambridge Centre for Proteomics, Cambridge Systems Biology Centre, Cambridge, UKen
dc.contributor.institutionDepartment of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UKen
kaust.grant.numberCRG3-62140383en
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