Handle URI:
http://hdl.handle.net/10754/623510
Title:
A structural view of microRNA–target recognition
Authors:
Leoni, Guido; Tramontano, Anna
Abstract:
It is well established that the correct identification of the messenger RNA targeted by a given microRNA (miRNA) is a difficult problem, and that available methods all suffer from low specificity. We hypothesize that the correct identification of the pairing should take into account the effect of the Argonaute protein (AGO), an essential catalyst of the recognition process. Therefore, we developed a strategy named MiREN for building and scoring three-dimensional models of the ternary complex formed by AGO, a miRNA and 22 nt of a target mRNA that putatively interacts with it. We show here that MiREN can be used to assess the likelihood that an RNA molecule is the target of a given miRNA and that this approach is more accurate than other existing methods, usually based on sequence or sequence-related features. Our results also suggest that AGO plays a relevant role in the selection of the miRNA targets. Our method can represent an additional step for refining predictions made by faster but less accurate classical methods for the identification of miRNA targets.
Citation:
Leoni G, Tramontano A (2016) A structural view of microRNA–target recognition. Nucleic Acids Research 44: e82–e82. Available: http://dx.doi.org/10.1093/nar/gkw043.
Publisher:
Oxford University Press (OUP)
Journal:
Nucleic Acids Research
KAUST Grant Number:
KUK-I1–012–43
Issue Date:
30-Jan-2016
DOI:
10.1093/nar/gkw043
Type:
Article
ISSN:
0305-1048; 1362-4962
Sponsors:
Epigenomics Flagship Project—EPIGEN; KAUST [KUK-I1–012–43]; PRIN Project [20108XYHJS]. Funding for open access charge: Epigenomics Flagship Project—EPIGEN.
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorLeoni, Guidoen
dc.contributor.authorTramontano, Annaen
dc.date.accessioned2017-05-15T10:35:05Z-
dc.date.available2017-05-15T10:35:05Z-
dc.date.issued2016-01-30en
dc.identifier.citationLeoni G, Tramontano A (2016) A structural view of microRNA–target recognition. Nucleic Acids Research 44: e82–e82. Available: http://dx.doi.org/10.1093/nar/gkw043.en
dc.identifier.issn0305-1048en
dc.identifier.issn1362-4962en
dc.identifier.doi10.1093/nar/gkw043en
dc.identifier.urihttp://hdl.handle.net/10754/623510-
dc.description.abstractIt is well established that the correct identification of the messenger RNA targeted by a given microRNA (miRNA) is a difficult problem, and that available methods all suffer from low specificity. We hypothesize that the correct identification of the pairing should take into account the effect of the Argonaute protein (AGO), an essential catalyst of the recognition process. Therefore, we developed a strategy named MiREN for building and scoring three-dimensional models of the ternary complex formed by AGO, a miRNA and 22 nt of a target mRNA that putatively interacts with it. We show here that MiREN can be used to assess the likelihood that an RNA molecule is the target of a given miRNA and that this approach is more accurate than other existing methods, usually based on sequence or sequence-related features. Our results also suggest that AGO plays a relevant role in the selection of the miRNA targets. Our method can represent an additional step for refining predictions made by faster but less accurate classical methods for the identification of miRNA targets.en
dc.description.sponsorshipEpigenomics Flagship Project—EPIGEN; KAUST [KUK-I1–012–43]; PRIN Project [20108XYHJS]. Funding for open access charge: Epigenomics Flagship Project—EPIGEN.en
dc.publisherOxford University Press (OUP)en
dc.subjectProtein-nucleic acid interactionen
dc.subjectComputational Methodsen
dc.titleA structural view of microRNA–target recognitionen
dc.typeArticleen
dc.identifier.journalNucleic Acids Researchen
dc.contributor.institutionDepartment of Physics, Sapienza University, Piazzale Aldo Moro, 5-00184 Rome, Italyen
dc.contributor.institutionIstituto Pasteur-Fondazione Cenci Bolognetti, Viale Regina Elena 291, 00161 Rome, Italyen
kaust.grant.numberKUK-I1–012–43en
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