Engineering Molecular Immunity Against Plant Viruses

Handle URI:
http://hdl.handle.net/10754/623451
Title:
Engineering Molecular Immunity Against Plant Viruses
Authors:
Zaidi, Syed Shan-e-Ali; Tashkandi, Manal; Mahfouz, Magdy M. ( 0000-0002-0616-6365 )
Abstract:
Genomic engineering has been used to precisely alter eukaryotic genomes at the single-base level for targeted gene editing, replacement, fusion, and mutagenesis, and plant viruses such as Tobacco rattle virus have been developed into efficient vectors for delivering genome-engineering reagents. In addition to altering the host genome, these methods can target pathogens to engineer molecular immunity. Indeed, recent studies have shown that clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) systems that target the genomes of DNA viruses can interfere with viral activity and limit viral symptoms in planta, demonstrating the utility of this system for engineering molecular immunity in plants. CRISPR/Cas9 can efficiently target single and multiple viral infections and confer plant immunity. Here, we discuss the use of site-specific nucleases to engineer molecular immunity against DNA and RNA viruses in plants. We also explore how to address the potential challenges encountered when producing plants with engineered resistance to single and mixed viral infections.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Plant Science Program; Desert Agriculture Initiative; Laboratory for Genome Engineering
Citation:
Zaidi SS-A, Tashkandi M, Mahfouz MM (2017) Engineering Molecular Immunity Against Plant Viruses. Gene Editing in Plants: 167–186. Available: http://dx.doi.org/10.1016/bs.pmbts.2017.03.009.
Publisher:
Elsevier
Journal:
Progress in Molecular Biology and Translational Science
Issue Date:
26-Apr-2017
DOI:
10.1016/bs.pmbts.2017.03.009
Type:
Book Chapter
ISSN:
1877-1173
Additional Links:
http://www.sciencedirect.com/science/article/pii/S1877117317300418
Appears in Collections:
Bioscience Program; Center for Desert Agriculture; Book Chapters; Plant Science Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorZaidi, Syed Shan-e-Alien
dc.contributor.authorTashkandi, Manalen
dc.contributor.authorMahfouz, Magdy M.en
dc.date.accessioned2017-05-09T12:54:46Z-
dc.date.available2017-05-09T12:54:46Z-
dc.date.issued2017-04-26en
dc.identifier.citationZaidi SS-A, Tashkandi M, Mahfouz MM (2017) Engineering Molecular Immunity Against Plant Viruses. Gene Editing in Plants: 167–186. Available: http://dx.doi.org/10.1016/bs.pmbts.2017.03.009.en
dc.identifier.issn1877-1173en
dc.identifier.doi10.1016/bs.pmbts.2017.03.009en
dc.identifier.urihttp://hdl.handle.net/10754/623451-
dc.description.abstractGenomic engineering has been used to precisely alter eukaryotic genomes at the single-base level for targeted gene editing, replacement, fusion, and mutagenesis, and plant viruses such as Tobacco rattle virus have been developed into efficient vectors for delivering genome-engineering reagents. In addition to altering the host genome, these methods can target pathogens to engineer molecular immunity. Indeed, recent studies have shown that clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) systems that target the genomes of DNA viruses can interfere with viral activity and limit viral symptoms in planta, demonstrating the utility of this system for engineering molecular immunity in plants. CRISPR/Cas9 can efficiently target single and multiple viral infections and confer plant immunity. Here, we discuss the use of site-specific nucleases to engineer molecular immunity against DNA and RNA viruses in plants. We also explore how to address the potential challenges encountered when producing plants with engineered resistance to single and mixed viral infections.en
dc.publisherElsevieren
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S1877117317300418en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Progress in Molecular Biology and Translational Science. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Progress in Molecular Biology and Translational Science, [, , (2017-04-26)] DOI: 10.1016/bs.pmbts.2017.03.009. © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectSite-specific nucleaseen
dc.subjectGenome engineeringen
dc.subjectCRISPR/Cas9en
dc.subjectGeminivirusesen
dc.subjectTobacco rattle virusen
dc.titleEngineering Molecular Immunity Against Plant Virusesen
dc.typeBook Chapteren
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentPlant Science Programen
dc.contributor.departmentDesert Agriculture Initiativeen
dc.contributor.departmentLaboratory for Genome Engineeringen
dc.identifier.journalProgress in Molecular Biology and Translational Scienceen
dc.eprint.versionPost-printen
dc.contributor.institutionNational Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistanen
kaust.authorZaidi, Syed Shan-e-Alien
kaust.authorTashkandi, Manalen
kaust.authorMahfouz, Magdy M.en
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