Prochlorococcus as a Possible Source for Transparent Exopolymer Particles (TEP)

Handle URI:
http://hdl.handle.net/10754/623327
Title:
Prochlorococcus as a Possible Source for Transparent Exopolymer Particles (TEP)
Authors:
Agusti, Susana ( 0000-0003-0536-7293 ) ; Iuculano, Francesca; Mazuecos, Ignacio P.; Reche, Isabel
Abstract:
Transparent exopolymer particles (TEP), usually associated with phytoplankton blooms, promote the formation of marine aggregates. Their exportation to deep waters is considered a key component of the biological carbon pump. Here, we explored the role of solar radiation and picocyanobacteria in the formation of TEP in oligotrophic surface waters of the Atlantic and Pacific Oceans in ten on-deck incubation experiments during the Malaspina 2010 Expedition. TEP concentrations were low on the ocean’s surface although these concentrations were significantly higher on the surface of the Pacific (24.45 ± 2.3 μg XG Eq. L-1) than on the surface of the Atlantic Ocean (8.18 ± 4.56 μg XG Eq. L-1). Solar radiation induced a significant production of TEP in the on-deck experiments from the surface water of the Pacific Ocean, reaching values up to 187.3 μg XG Eq. L-1 compared with the low production observed in the dark controls. By contrast, TEP production in the Atlantic Ocean experiments was lower, and its formation was not related to the light treatments. Prochlorococcus sp. from the surface ocean was very sensitive to solar radiation and experienced a high cell decay in the Pacific Ocean experiments. TEP production in the on-deck incubation experiments was closely related to the observed cell decay rates of Prochlorococcus sp., suggesting that this picocyanobacteria genus is a potential source of TEP. The evidence to propose such potential role was derived experimentally, using natural communities including the presence of several species and a variety of processes. Laboratory experiments with cultures of a non-axenic strain of Prochlorococcus marinus were then used to test TEP production by this genus. TEP concentrations in the culture increased with increasing cell abundance during the exponential phase, reaching the highest TEP concentration at the beginning of the stationary phase. The average TEP concentration of 1474 ± 226 μg XG Eq. L-1 (mean ± SE) observed at the stationary phase of P. marinus cultures is comparable with the values reported in the literature for diatom cultures, also growing in non-axenic as well as axenic cultures. Our results identify Prochlorococcus sp. as a possible relevant source of TEP in the oligotrophic ocean.
KAUST Department:
Red Sea Research Center (RSRC)
Citation:
Agustí S, Iuculano F, Mazuecos IP, Reche I (2017) Prochlorococcus as a Possible Source for Transparent Exopolymer Particles (TEP). Frontiers in Microbiology 8. Available: http://dx.doi.org/10.3389/fmicb.2017.00709.
Publisher:
Frontiers Media SA
Journal:
Frontiers in Microbiology
Issue Date:
26-Apr-2017
DOI:
10.3389/fmicb.2017.00709
Type:
Article
ISSN:
1664-302X
Sponsors:
This research was funded by the Expedición Malaspina 2010 project of the CONSOLIDER program (ref. CSD2008-00077), the project Stress-X (ref. CTM2012-32603) of the Spanish Minister of Economy and Competitiveness, and King Abdullah University of Science and Technology baseline funding to SA. FI was supported by a CSIC JAE-preDOC fellowship.
Additional Links:
http://journal.frontiersin.org/article/10.3389/fmicb.2017.00709/full
Appears in Collections:
Articles; Red Sea Research Center (RSRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorAgusti, Susanaen
dc.contributor.authorIuculano, Francescaen
dc.contributor.authorMazuecos, Ignacio P.en
dc.contributor.authorReche, Isabelen
dc.date.accessioned2017-05-04T06:39:21Z-
dc.date.available2017-05-04T06:39:21Z-
dc.date.issued2017-04-26en
dc.identifier.citationAgustí S, Iuculano F, Mazuecos IP, Reche I (2017) Prochlorococcus as a Possible Source for Transparent Exopolymer Particles (TEP). Frontiers in Microbiology 8. Available: http://dx.doi.org/10.3389/fmicb.2017.00709.en
dc.identifier.issn1664-302Xen
dc.identifier.doi10.3389/fmicb.2017.00709en
dc.identifier.urihttp://hdl.handle.net/10754/623327-
dc.description.abstractTransparent exopolymer particles (TEP), usually associated with phytoplankton blooms, promote the formation of marine aggregates. Their exportation to deep waters is considered a key component of the biological carbon pump. Here, we explored the role of solar radiation and picocyanobacteria in the formation of TEP in oligotrophic surface waters of the Atlantic and Pacific Oceans in ten on-deck incubation experiments during the Malaspina 2010 Expedition. TEP concentrations were low on the ocean’s surface although these concentrations were significantly higher on the surface of the Pacific (24.45 ± 2.3 μg XG Eq. L-1) than on the surface of the Atlantic Ocean (8.18 ± 4.56 μg XG Eq. L-1). Solar radiation induced a significant production of TEP in the on-deck experiments from the surface water of the Pacific Ocean, reaching values up to 187.3 μg XG Eq. L-1 compared with the low production observed in the dark controls. By contrast, TEP production in the Atlantic Ocean experiments was lower, and its formation was not related to the light treatments. Prochlorococcus sp. from the surface ocean was very sensitive to solar radiation and experienced a high cell decay in the Pacific Ocean experiments. TEP production in the on-deck incubation experiments was closely related to the observed cell decay rates of Prochlorococcus sp., suggesting that this picocyanobacteria genus is a potential source of TEP. The evidence to propose such potential role was derived experimentally, using natural communities including the presence of several species and a variety of processes. Laboratory experiments with cultures of a non-axenic strain of Prochlorococcus marinus were then used to test TEP production by this genus. TEP concentrations in the culture increased with increasing cell abundance during the exponential phase, reaching the highest TEP concentration at the beginning of the stationary phase. The average TEP concentration of 1474 ± 226 μg XG Eq. L-1 (mean ± SE) observed at the stationary phase of P. marinus cultures is comparable with the values reported in the literature for diatom cultures, also growing in non-axenic as well as axenic cultures. Our results identify Prochlorococcus sp. as a possible relevant source of TEP in the oligotrophic ocean.en
dc.description.sponsorshipThis research was funded by the Expedición Malaspina 2010 project of the CONSOLIDER program (ref. CSD2008-00077), the project Stress-X (ref. CTM2012-32603) of the Spanish Minister of Economy and Competitiveness, and King Abdullah University of Science and Technology baseline funding to SA. FI was supported by a CSIC JAE-preDOC fellowship.en
dc.publisherFrontiers Media SAen
dc.relation.urlhttp://journal.frontiersin.org/article/10.3389/fmicb.2017.00709/fullen
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleProchlorococcus as a Possible Source for Transparent Exopolymer Particles (TEP)en
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalFrontiers in Microbiologyen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Global Change, Instituto Mediterráneo de Estudios Avanzados, Consejo Superior de Investigaciones Cientificas – Universitat de les Illes Balears, Esporles, Spainen
dc.contributor.institutionDepartamento de Ecología, Instituto del Agua, Universidad de Granada, Granada, Spainen
kaust.authorAgusti, Susanaen
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