Laboratory-Cultured Strains of the Sea Anemone Exaiptasia Reveal Distinct Bacterial Communities

Handle URI:
http://hdl.handle.net/10754/623326
Title:
Laboratory-Cultured Strains of the Sea Anemone Exaiptasia Reveal Distinct Bacterial Communities
Authors:
Herrera Sarrias, Marcela ( 0000-0001-6021-3989 ) ; Ziegler, Maren ( 0000-0003-2237-9261 ) ; Voolstra, Christian R. ( 0000-0003-4555-3795 ) ; Aranda, Manuel ( 0000-0001-6673-016X )
Abstract:
Exaiptasia is a laboratory sea anemone model system for stony corals. Two clonal strains are commonly used, referred to as H2 and CC7, that originate from two genetically distinct lineages and that differ in their Symbiodinium specificity. However, little is known about their other microbial associations. Here, we examined and compared the taxonomic composition of the bacterial assemblages of these two symbiotic Exaiptasia strains, both of which have been cultured in the laboratory long-term under identical conditions. We found distinct bacterial microbiota for each strain, indicating the presence of host-specific microbial consortia. Putative differences in the bacterial functional profiles (i.e., enrichment and depletion of various metabolic processes) based on taxonomic inference were also detected, further suggesting functional differences of the microbiomes associated with these lineages. Our study contributes to the current knowledge of the Exaiptasia holobiont by comparing the bacterial diversity of two commonly used strains as models for coral research.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Red Sea Research Center (RSRC)
Citation:
Herrera M, Ziegler M, Voolstra CR, Aranda M (2017) Laboratory-Cultured Strains of the Sea Anemone Exaiptasia Reveal Distinct Bacterial Communities. Frontiers in Marine Science 4. Available: http://dx.doi.org/10.3389/fmars.2017.00115.
Publisher:
Frontiers Media SA
Journal:
Frontiers in Marine Science
Issue Date:
2-May-2017
DOI:
10.3389/fmars.2017.00115
Type:
Article
ISSN:
2296-7745
Sponsors:
This study was supported by KAUST baseline research funds to MA and CRV. We thank Craig Michell for MiSeq sequencing library generation, Till Röthig, Yi Jin Liew, and Shobhit Agrawal for helpful discussions that greatly improved the manuscript.
Additional Links:
http://journal.frontiersin.org/article/10.3389/fmars.2017.00115/full
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division; Reef Genomics, part of the Global Ocean Genome Project

Full metadata record

DC FieldValue Language
dc.contributor.authorHerrera Sarrias, Marcelaen
dc.contributor.authorZiegler, Marenen
dc.contributor.authorVoolstra, Christian R.en
dc.contributor.authorAranda, Manuelen
dc.date.accessioned2017-05-04T06:39:21Z-
dc.date.available2017-05-04T06:39:21Z-
dc.date.issued2017-05-02en
dc.identifier.citationHerrera M, Ziegler M, Voolstra CR, Aranda M (2017) Laboratory-Cultured Strains of the Sea Anemone Exaiptasia Reveal Distinct Bacterial Communities. Frontiers in Marine Science 4. Available: http://dx.doi.org/10.3389/fmars.2017.00115.en
dc.identifier.issn2296-7745en
dc.identifier.doi10.3389/fmars.2017.00115en
dc.identifier.urihttp://hdl.handle.net/10754/623326-
dc.description.abstractExaiptasia is a laboratory sea anemone model system for stony corals. Two clonal strains are commonly used, referred to as H2 and CC7, that originate from two genetically distinct lineages and that differ in their Symbiodinium specificity. However, little is known about their other microbial associations. Here, we examined and compared the taxonomic composition of the bacterial assemblages of these two symbiotic Exaiptasia strains, both of which have been cultured in the laboratory long-term under identical conditions. We found distinct bacterial microbiota for each strain, indicating the presence of host-specific microbial consortia. Putative differences in the bacterial functional profiles (i.e., enrichment and depletion of various metabolic processes) based on taxonomic inference were also detected, further suggesting functional differences of the microbiomes associated with these lineages. Our study contributes to the current knowledge of the Exaiptasia holobiont by comparing the bacterial diversity of two commonly used strains as models for coral research.en
dc.description.sponsorshipThis study was supported by KAUST baseline research funds to MA and CRV. We thank Craig Michell for MiSeq sequencing library generation, Till Röthig, Yi Jin Liew, and Shobhit Agrawal for helpful discussions that greatly improved the manuscript.en
dc.publisherFrontiers Media SAen
dc.relation.urlhttp://journal.frontiersin.org/article/10.3389/fmars.2017.00115/fullen
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleLaboratory-Cultured Strains of the Sea Anemone Exaiptasia Reveal Distinct Bacterial Communitiesen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalFrontiers in Marine Scienceen
dc.eprint.versionPublisher's Version/PDFen
kaust.authorHerrera Sarrias, Marcelaen
kaust.authorZiegler, Marenen
kaust.authorVoolstra, Christian R.en
kaust.authorAranda, Manuelen
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