Role of MCT1 and CAII in skeletal muscle pH homeostasis, energetics, and function: in vivo insights from MCT1 haploinsufficient mice

Handle URI:
http://hdl.handle.net/10754/623204
Title:
Role of MCT1 and CAII in skeletal muscle pH homeostasis, energetics, and function: in vivo insights from MCT1 haploinsufficient mice
Authors:
Chatel, Benjamin; Bendahan, David; Hourdé, Christophe; Pellerin, Luc; Lengacher, Sylvain; Magistretti, Pierre J. ( 0000-0002-6678-320X ) ; Le Fur, Yann; Vilmen, Christophe; Bernard, Monique; Messonnier, Laurent A.
Abstract:
The purpose of this study was to investigate the effects of a partial suppression of monocarboxylate transporter (MCT)-1 on skeletal muscle pH, energetics, and function (MCT1(+/-) mice). Twenty-four MCT1(+/-) and 13 wild-type (WT) mice were subjected to a rest-exercise-recovery protocol, allowing assessment of muscle energetics (by magnetic resonance spectroscopy) and function. The study included analysis of enzyme activities and content of protein involved in pH regulation. Skeletal muscle of MCT1(+/-) mice had lower MCT1 (-61%; P < 0.05) and carbonic anhydrase (CA)-II (-54%; P < 0.05) contents. Although intramuscular pH was higher in MCT1(+/-) mice at rest (P < 0.001), the mice showed higher acidosis during the first minute of exercise (P < 0.01). Then, the pH time course was similar among groups until exercise completion. MCT1(+/-) mice had higher specific peak (P < 0.05) and maximum tetanic (P < 0.01) forces and lower fatigability (P < 0.001) when compared to WT mice. We conclude that both MCT1 and CAII are involved in the homeostatic control of pH in skeletal muscle, both at rest and at the onset of exercise. The improved muscle function and resistance to fatigue in MCT1(+/-) mice remain unexplained.-Chatel, B., Bendahan, D., Hourdé, C., Pellerin, L., Lengacher, S., Magistretti, P., Fur, Y. L., Vilmen, C., Bernard, M., Messonnier, L. A. Role of MCT1 and CAII in skeletal muscle pH homeostasis, energetics, and function: in vivo insights from MCT1 haploinsufficient mice.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Chatel B, Bendahan D, Hourdé C, Pellerin L, Lengacher S, et al. (2017) Role of MCT1 and CAII in skeletal muscle pH homeostasis, energetics, and function: in vivo insights from MCT1 haploinsufficient mice. The FASEB Journal: fj.201601259R. Available: http://dx.doi.org/10.1096/fj.201601259r.
Publisher:
FASEB
Journal:
The FASEB Journal
Issue Date:
3-Mar-2017
DOI:
10.1096/fj.201601259r
Type:
Article
ISSN:
0892-6638; 1530-6860
Additional Links:
http://www.fasebj.org/content/early/2017/03/02/fj.201601259R
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorChatel, Benjaminen
dc.contributor.authorBendahan, Daviden
dc.contributor.authorHourdé, Christopheen
dc.contributor.authorPellerin, Lucen
dc.contributor.authorLengacher, Sylvainen
dc.contributor.authorMagistretti, Pierre J.en
dc.contributor.authorLe Fur, Yannen
dc.contributor.authorVilmen, Christopheen
dc.contributor.authorBernard, Moniqueen
dc.contributor.authorMessonnier, Laurent A.en
dc.date.accessioned2017-04-13T11:51:01Z-
dc.date.available2017-04-13T11:51:01Z-
dc.date.issued2017-03-03en
dc.identifier.citationChatel B, Bendahan D, Hourdé C, Pellerin L, Lengacher S, et al. (2017) Role of MCT1 and CAII in skeletal muscle pH homeostasis, energetics, and function: in vivo insights from MCT1 haploinsufficient mice. The FASEB Journal: fj.201601259R. Available: http://dx.doi.org/10.1096/fj.201601259r.en
dc.identifier.issn0892-6638en
dc.identifier.issn1530-6860en
dc.identifier.doi10.1096/fj.201601259ren
dc.identifier.urihttp://hdl.handle.net/10754/623204-
dc.description.abstractThe purpose of this study was to investigate the effects of a partial suppression of monocarboxylate transporter (MCT)-1 on skeletal muscle pH, energetics, and function (MCT1(+/-) mice). Twenty-four MCT1(+/-) and 13 wild-type (WT) mice were subjected to a rest-exercise-recovery protocol, allowing assessment of muscle energetics (by magnetic resonance spectroscopy) and function. The study included analysis of enzyme activities and content of protein involved in pH regulation. Skeletal muscle of MCT1(+/-) mice had lower MCT1 (-61%; P < 0.05) and carbonic anhydrase (CA)-II (-54%; P < 0.05) contents. Although intramuscular pH was higher in MCT1(+/-) mice at rest (P < 0.001), the mice showed higher acidosis during the first minute of exercise (P < 0.01). Then, the pH time course was similar among groups until exercise completion. MCT1(+/-) mice had higher specific peak (P < 0.05) and maximum tetanic (P < 0.01) forces and lower fatigability (P < 0.001) when compared to WT mice. We conclude that both MCT1 and CAII are involved in the homeostatic control of pH in skeletal muscle, both at rest and at the onset of exercise. The improved muscle function and resistance to fatigue in MCT1(+/-) mice remain unexplained.-Chatel, B., Bendahan, D., Hourdé, C., Pellerin, L., Lengacher, S., Magistretti, P., Fur, Y. L., Vilmen, C., Bernard, M., Messonnier, L. A. Role of MCT1 and CAII in skeletal muscle pH homeostasis, energetics, and function: in vivo insights from MCT1 haploinsufficient mice.en
dc.publisherFASEBen
dc.relation.urlhttp://www.fasebj.org/content/early/2017/03/02/fj.201601259Ren
dc.subjectmagnetic resonance spectroscopyen
dc.subjectCarbonic Anhydrasesen
dc.subjectMonocarboxylate Transportersen
dc.titleRole of MCT1 and CAII in skeletal muscle pH homeostasis, energetics, and function: in vivo insights from MCT1 haploinsufficient miceen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalThe FASEB Journalen
dc.contributor.institutionCentre de Résonance Magnétique Biologique et Médicale, Centre National de la Recherche Scientifique, Aix-Marseille Université, Marseille, Franceen
dc.contributor.institutionbenjamin.chatel@live.fr.en
dc.contributor.institutionCentre de Résonance Magnétique Biologique et Médicale, Centre National de la Recherche Scientifique, Aix-Marseille Université, Marseille, France.en
dc.contributor.institutionLaboratoire Interuniversitaire de Biologie de la Motricité, Université Savoie Mont Blanc, Chambéry, France.en
dc.contributor.institutionDepartment of Physiology, University of Lausanne, Lausanne, Switzerland.en
dc.contributor.institutionLaboratory of Neuroenergetic and Cellular Dynamics, Brain and Mind Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.en
kaust.authorMagistretti, Pierre J.en
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