Cardiac Progenitor Cell Extraction from Human Auricles

Handle URI:
http://hdl.handle.net/10754/623194
Title:
Cardiac Progenitor Cell Extraction from Human Auricles
Authors:
Di Nardo, Paolo; Pagliari, Francesca ( 0000-0002-5547-222X )
Abstract:
For many years, myocardial tissue has been considered terminally differentiated and, thus, incapable of regenerating. Recent studies have shown, instead, that cardiomyocytes, at least in part, are slowly substituted by new cells originating by precursor cells mostly embedded into the heart apex and in the atria. We have shown that an elective region of progenitor cell embedding is represented by the auricles, non-contractile atria appendages that can be easily sampled without harming the patient. The protocol here reported describes how from auricles a population of multipotent, cardiogenic cells can be isolated, cultured, and differentiated. Further studies are needed to fully exploit this cell population, but, sampling auricles, it could be possible to treat cardiac patients using their own cells circumventing rejection or organ shortage limitations.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Di Nardo P, Pagliari F (2017) Cardiac Progenitor Cell Extraction from Human Auricles. Methods in Molecular Biology: 145–154. Available: http://dx.doi.org/10.1007/978-1-4939-6756-8_11.
Publisher:
Springer Nature
Journal:
Methods in Molecular Biology
Issue Date:
22-Feb-2017
DOI:
10.1007/978-1-4939-6756-8_11
Type:
Book Chapter
ISSN:
1064-3745; 1940-6029
Additional Links:
http://link.springer.com/chapter/10.1007/978-1-4939-6756-8_11
Appears in Collections:
Book Chapters; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorDi Nardo, Paoloen
dc.contributor.authorPagliari, Francescaen
dc.date.accessioned2017-04-13T11:51:00Z-
dc.date.available2017-04-13T11:51:00Z-
dc.date.issued2017-02-22en
dc.identifier.citationDi Nardo P, Pagliari F (2017) Cardiac Progenitor Cell Extraction from Human Auricles. Methods in Molecular Biology: 145–154. Available: http://dx.doi.org/10.1007/978-1-4939-6756-8_11.en
dc.identifier.issn1064-3745en
dc.identifier.issn1940-6029en
dc.identifier.doi10.1007/978-1-4939-6756-8_11en
dc.identifier.urihttp://hdl.handle.net/10754/623194-
dc.description.abstractFor many years, myocardial tissue has been considered terminally differentiated and, thus, incapable of regenerating. Recent studies have shown, instead, that cardiomyocytes, at least in part, are slowly substituted by new cells originating by precursor cells mostly embedded into the heart apex and in the atria. We have shown that an elective region of progenitor cell embedding is represented by the auricles, non-contractile atria appendages that can be easily sampled without harming the patient. The protocol here reported describes how from auricles a population of multipotent, cardiogenic cells can be isolated, cultured, and differentiated. Further studies are needed to fully exploit this cell population, but, sampling auricles, it could be possible to treat cardiac patients using their own cells circumventing rejection or organ shortage limitations.en
dc.publisherSpringer Natureen
dc.relation.urlhttp://link.springer.com/chapter/10.1007/978-1-4939-6756-8_11en
dc.subjectHearten
dc.subjectCardiac progenitor cellsen
dc.subjectDifferentiationen
dc.subjectSCA-1en
dc.subjectImmunomagnetic separationen
dc.titleCardiac Progenitor Cell Extraction from Human Auriclesen
dc.typeBook Chapteren
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalMethods in Molecular Biologyen
dc.contributor.institutionCenter for Regenerative Medicine, Università di Roma Tor Vergata, Via Orazio Raimondo, 00173, Rome, Italyen
dc.contributor.institutionDepartment of Clinical Sciences and Translational Medicine, Università di Roma Tor Vergata, Via Orazio Raimondo, 00173, Rome, Italyen
kaust.authorPagliari, Francescaen
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