Flow cytometry detection of planktonic cells with polycyclic aromatic hydrocarbons sorbed to cell surfaces

Handle URI:
http://hdl.handle.net/10754/623190
Title:
Flow cytometry detection of planktonic cells with polycyclic aromatic hydrocarbons sorbed to cell surfaces
Authors:
Cerezo, Maria I.; Linden, Matthew; Agusti, Susana ( 0000-0003-0536-7293 )
Abstract:
Polycyclic aromatic hydrocarbons are very important components of oil pollution. These pollutants tend to sorb to cell surfaces, exerting toxic effects on organisms. Our study developed a flow cytometric method for the detection of PAHs sorbed to phytoplankton by exploiting their spectral characteristics. We discriminated between cells with PAHs from cells free of PAHs. Clear discrimination was observed with flow cytometer provided with 375 or 405nm lasers in addition to the standard 488nm laser necessary to identify phytoplankton. Using this method, we measured the relationship between the percentages of phytoplankton organisms with PAHs, with the decrease in the growth rate. Moreover, the development of this method could be extended to facilitate the study of PAHs impact on cell cultures from a large variety of organisms.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Red Sea Research Center (RSRC)
Citation:
Cerezo MI, Linden M, Agustí S (2017) Flow cytometry detection of planktonic cells with polycyclic aromatic hydrocarbons sorbed to cell surfaces. Marine Pollution Bulletin 118: 64–70. Available: http://dx.doi.org/10.1016/j.marpolbul.2017.02.006.
Publisher:
Elsevier BV
Journal:
Marine Pollution Bulletin
Issue Date:
17-Feb-2017
DOI:
10.1016/j.marpolbul.2017.02.006
Type:
Article
ISSN:
0025-326X
Sponsors:
This research was supported by the Spanish Ministry of Economy and Competitiveness by the project StressX (CTM2012-32603) and the Australian Research Council's Discovery Projects (project number DP140100825). M.I. Cerezo acknowledges a pre-doctoral fellow-ship from the JAE program (JAEPre121) of the Consejo Superior de Investigaciones Cientificas (CSIC). The authors acknowledge the facilities, and the scientific and technical assistance of the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy, Characterization & Analysis, the University of Western Australia, a facility funded by the University, State and Commonwealth Governments.
Additional Links:
http://www.sciencedirect.com/science/article/pii/S0025326X17301236
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorCerezo, Maria I.en
dc.contributor.authorLinden, Matthewen
dc.contributor.authorAgusti, Susanaen
dc.date.accessioned2017-04-13T11:51:00Z-
dc.date.available2017-04-13T11:51:00Z-
dc.date.issued2017-02-17en
dc.identifier.citationCerezo MI, Linden M, Agustí S (2017) Flow cytometry detection of planktonic cells with polycyclic aromatic hydrocarbons sorbed to cell surfaces. Marine Pollution Bulletin 118: 64–70. Available: http://dx.doi.org/10.1016/j.marpolbul.2017.02.006.en
dc.identifier.issn0025-326Xen
dc.identifier.doi10.1016/j.marpolbul.2017.02.006en
dc.identifier.urihttp://hdl.handle.net/10754/623190-
dc.description.abstractPolycyclic aromatic hydrocarbons are very important components of oil pollution. These pollutants tend to sorb to cell surfaces, exerting toxic effects on organisms. Our study developed a flow cytometric method for the detection of PAHs sorbed to phytoplankton by exploiting their spectral characteristics. We discriminated between cells with PAHs from cells free of PAHs. Clear discrimination was observed with flow cytometer provided with 375 or 405nm lasers in addition to the standard 488nm laser necessary to identify phytoplankton. Using this method, we measured the relationship between the percentages of phytoplankton organisms with PAHs, with the decrease in the growth rate. Moreover, the development of this method could be extended to facilitate the study of PAHs impact on cell cultures from a large variety of organisms.en
dc.description.sponsorshipThis research was supported by the Spanish Ministry of Economy and Competitiveness by the project StressX (CTM2012-32603) and the Australian Research Council's Discovery Projects (project number DP140100825). M.I. Cerezo acknowledges a pre-doctoral fellow-ship from the JAE program (JAEPre121) of the Consejo Superior de Investigaciones Cientificas (CSIC). The authors acknowledge the facilities, and the scientific and technical assistance of the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy, Characterization & Analysis, the University of Western Australia, a facility funded by the University, State and Commonwealth Governments.en
dc.publisherElsevier BVen
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0025326X17301236en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Marine Pollution Bulletin. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Marine Pollution Bulletin, [, , (2017-02-17)] DOI: 10.1016/j.marpolbul.2017.02.006 . © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectPAHsen
dc.subjectFlow cytometryen
dc.subjectPhytoplanktonen
dc.subjectOil spillsen
dc.subjectCell fluorescenceen
dc.subjectUltraviolet laseren
dc.titleFlow cytometry detection of planktonic cells with polycyclic aromatic hydrocarbons sorbed to cell surfacesen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalMarine Pollution Bulletinen
dc.eprint.versionPost-printen
dc.contributor.institutionDepartment of Global Change Research, IMEDEA (CSIC-UIB), Instituto Mediterráneo de Estudios Avanzados, Miquel Marqués 21, 07190 Esporles, Spainen
dc.contributor.institutionCentre for Microscopy, Characterisation and Analysis (CMCA), The University of Western Australia, Mail Bag 510, 35 Stirling Highway, Crawley 6009, WA, Australiaen
kaust.authorAgusti, Susanaen
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