Performance and microbial community variations of anaerobic digesters under increasing tetracycline concentrations

Handle URI:
http://hdl.handle.net/10754/623100
Title:
Performance and microbial community variations of anaerobic digesters under increasing tetracycline concentrations
Authors:
Xiong, Yanghui ( 0000-0002-5009-547X ) ; Harb, Moustapha ( 0000-0002-1566-987X ) ; Hong, Pei-Ying ( 0000-0002-4474-6600 )
Abstract:
The impact of different concentrations of tetracycline on the performance of anaerobic treatment was evaluated. Results revealed that for all of the tested tetracycline concentrations, no major sustained impact on methane production was observed. Instead, a significant increase in propionic acid was observed in the reactor subjected to the highest concentration of tetracycline (20 mg/L). Microbial community analyses suggest that an alternative methanogenic pathway, specifically that of methanol-utilizing methanogens, may be important for ensuring the stability of methane production in the presence of high tetracycline concentrations. In addition, the accumulation of propionate was due to an increase in volatile fatty acids (VFA)-producing bacteria coupled with a reduction in propionate utilizers. An increase in the abundance of tetracycline resistance genes associated with ribosomal protection proteins was observed after 30 days of exposure to high concentrations of tetracycline, while other targeted resistance genes showed no significant changes. These findings suggest that anaerobic treatment processes can robustly treat wastewater with varying concentrations of antibiotics while also deriving value-added products and minimizing the dissemination of associated antibiotic resistance genes.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Water Desalination and Reuse Research Center (WDRC)
Citation:
Xiong Y, Harb M, Hong P-Y (2017) Performance and microbial community variations of anaerobic digesters under increasing tetracycline concentrations. Applied Microbiology and Biotechnology. Available: http://dx.doi.org/10.1007/s00253-017-8253-1.
Publisher:
Springer Nature
Journal:
Applied Microbiology and Biotechnology
KAUST Grant Number:
FCC/1/1971-06-01
Issue Date:
1-Apr-2017
DOI:
10.1007/s00253-017-8253-1
Type:
Article
ISSN:
0175-7598; 1432-0614
Sponsors:
The research reported in this publication was supported by the KAUST Center Competitive Funding Program grant (FCC/1/1971-06-01) awarded to P-Y Hong.
Additional Links:
http://link.springer.com/article/10.1007%2Fs00253-017-8253-1
Appears in Collections:
Articles; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorXiong, Yanghuien
dc.contributor.authorHarb, Moustaphaen
dc.contributor.authorHong, Pei-Yingen
dc.date.accessioned2017-04-10T07:49:51Z-
dc.date.available2017-04-10T07:49:51Z-
dc.date.issued2017-04-01en
dc.identifier.citationXiong Y, Harb M, Hong P-Y (2017) Performance and microbial community variations of anaerobic digesters under increasing tetracycline concentrations. Applied Microbiology and Biotechnology. Available: http://dx.doi.org/10.1007/s00253-017-8253-1.en
dc.identifier.issn0175-7598en
dc.identifier.issn1432-0614en
dc.identifier.doi10.1007/s00253-017-8253-1en
dc.identifier.urihttp://hdl.handle.net/10754/623100-
dc.description.abstractThe impact of different concentrations of tetracycline on the performance of anaerobic treatment was evaluated. Results revealed that for all of the tested tetracycline concentrations, no major sustained impact on methane production was observed. Instead, a significant increase in propionic acid was observed in the reactor subjected to the highest concentration of tetracycline (20 mg/L). Microbial community analyses suggest that an alternative methanogenic pathway, specifically that of methanol-utilizing methanogens, may be important for ensuring the stability of methane production in the presence of high tetracycline concentrations. In addition, the accumulation of propionate was due to an increase in volatile fatty acids (VFA)-producing bacteria coupled with a reduction in propionate utilizers. An increase in the abundance of tetracycline resistance genes associated with ribosomal protection proteins was observed after 30 days of exposure to high concentrations of tetracycline, while other targeted resistance genes showed no significant changes. These findings suggest that anaerobic treatment processes can robustly treat wastewater with varying concentrations of antibiotics while also deriving value-added products and minimizing the dissemination of associated antibiotic resistance genes.en
dc.description.sponsorshipThe research reported in this publication was supported by the KAUST Center Competitive Funding Program grant (FCC/1/1971-06-01) awarded to P-Y Hong.en
dc.publisherSpringer Natureen
dc.relation.urlhttp://link.springer.com/article/10.1007%2Fs00253-017-8253-1en
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectTetracyclineen
dc.subjectAnaerobic digestionen
dc.subjectPropionic aciden
dc.subjectTetracycline Resistance Genesen
dc.subjectMethanogenic Pathwayen
dc.titlePerformance and microbial community variations of anaerobic digesters under increasing tetracycline concentrationsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.identifier.journalApplied Microbiology and Biotechnologyen
dc.eprint.versionPublisher's Version/PDFen
kaust.authorXiong, Yanghuien
kaust.authorHarb, Moustaphaen
kaust.authorHong, Pei-Yingen
kaust.grant.numberFCC/1/1971-06-01en
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