Herboxidiene triggers splicing repression and abiotic stress responses in plants

Handle URI:
http://hdl.handle.net/10754/623088
Title:
Herboxidiene triggers splicing repression and abiotic stress responses in plants
Authors:
Alshareef, Sahar ( 0000-0002-0377-1113 ) ; Ling, Yu ( 0000-0002-8067-523X ) ; Butt, Haroon; Mariappan, Kiruthiga G.; Benhamed, Moussa ( 0000-0002-4181-1702 ) ; Mahfouz, Magdy M. ( 0000-0002-0616-6365 )
Abstract:
Background Constitutive and alternative splicing of pre-mRNAs from multiexonic genes controls the diversity of the proteome; these precisely regulated processes also fine-tune responses to cues related to growth, development, and stresses. Small-molecule inhibitors that perturb splicing provide invaluable tools for use as chemical probes to uncover the molecular underpinnings of splicing regulation and as potential anticancer compounds. Results Here, we show that herboxidiene (GEX1A) inhibits both constitutive and alternative splicing. Moreover, GEX1A activates genome-wide transcriptional patterns involved in abiotic stress responses in plants. GEX1A treatment -activated ABA-inducible promoters, and led to stomatal closure. Interestingly, GEX1A and pladienolide B (PB) elicited similar cellular changes, including alterations in the patterns of transcription and splicing, suggesting that these compounds might target the same spliceosome complex in plant cells. Conclusions Our study establishes GEX1A as a potent splicing inhibitor in plants that can be used to probe the assembly, dynamics, and molecular functions of the spliceosome and to study the interplay between splicing stress and abiotic stresses, as well as having potential biotechnological applications.
KAUST Department:
Laboratory for Genome Engineering, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology
Citation:
AlShareef S, Ling Y, Butt H, Mariappan KG, Benhamed M, et al. (2017) Herboxidiene triggers splicing repression and abiotic stress responses in plants. BMC Genomics 18. Available: http://dx.doi.org/10.1186/s12864-017-3656-z.
Publisher:
Springer Nature
Journal:
BMC Genomics
Issue Date:
27-Mar-2017
DOI:
10.1186/s12864-017-3656-z
Type:
Article
ISSN:
1471-2164
Sponsors:
We wish to thank members of the Laboratory for Genome Engineering at King Abdullah University of Science and Technology for helpful discussions and comments on the manuscript. We wish to thank Pedro Rodriguez for providing Arabidopsis seeds for several ABA mutants. We wish to thank Paula Duque for providing Arabidopsis seeds for several sr45-1 mutants. We wish to thank Sean Cutler for providing Arabidopsis seeds of MAPKKK18
Is Supplemented By:
AlShareef, S., Ling, Y., Butt, H., Kiruthiga Mariappan, Benhamed, M., & Magdy Mahfouz. (2017). Herboxidiene triggers splicing repression and abiotic stress responses in plants. Figshare. https://doi.org/10.6084/m9.figshare.c.3727597; DOI:10.6084/m9.figshare.c.3727597; HANDLE:http://hdl.handle.net/10754/624149
Additional Links:
http://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-017-3656-z
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorAlshareef, Saharen
dc.contributor.authorLing, Yuen
dc.contributor.authorButt, Haroonen
dc.contributor.authorMariappan, Kiruthiga G.en
dc.contributor.authorBenhamed, Moussaen
dc.contributor.authorMahfouz, Magdy M.en
dc.date.accessioned2017-04-10T07:49:50Z-
dc.date.available2017-04-10T07:49:50Z-
dc.date.issued2017-03-27en
dc.identifier.citationAlShareef S, Ling Y, Butt H, Mariappan KG, Benhamed M, et al. (2017) Herboxidiene triggers splicing repression and abiotic stress responses in plants. BMC Genomics 18. Available: http://dx.doi.org/10.1186/s12864-017-3656-z.en
dc.identifier.issn1471-2164en
dc.identifier.doi10.1186/s12864-017-3656-zen
dc.identifier.urihttp://hdl.handle.net/10754/623088-
dc.description.abstractBackground Constitutive and alternative splicing of pre-mRNAs from multiexonic genes controls the diversity of the proteome; these precisely regulated processes also fine-tune responses to cues related to growth, development, and stresses. Small-molecule inhibitors that perturb splicing provide invaluable tools for use as chemical probes to uncover the molecular underpinnings of splicing regulation and as potential anticancer compounds. Results Here, we show that herboxidiene (GEX1A) inhibits both constitutive and alternative splicing. Moreover, GEX1A activates genome-wide transcriptional patterns involved in abiotic stress responses in plants. GEX1A treatment -activated ABA-inducible promoters, and led to stomatal closure. Interestingly, GEX1A and pladienolide B (PB) elicited similar cellular changes, including alterations in the patterns of transcription and splicing, suggesting that these compounds might target the same spliceosome complex in plant cells. Conclusions Our study establishes GEX1A as a potent splicing inhibitor in plants that can be used to probe the assembly, dynamics, and molecular functions of the spliceosome and to study the interplay between splicing stress and abiotic stresses, as well as having potential biotechnological applications.en
dc.description.sponsorshipWe wish to thank members of the Laboratory for Genome Engineering at King Abdullah University of Science and Technology for helpful discussions and comments on the manuscript. We wish to thank Pedro Rodriguez for providing Arabidopsis seeds for several ABA mutants. We wish to thank Paula Duque for providing Arabidopsis seeds for several sr45-1 mutants. We wish to thank Sean Cutler for providing Arabidopsis seeds of MAPKKK18en
dc.publisherSpringer Natureen
dc.relation.urlhttp://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-017-3656-zen
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectAlternative splicingen
dc.subjectGEX1Aen
dc.subjectPladienolide Ben
dc.subjectSplicing inhibitorsen
dc.subjectSR proteinsen
dc.subjectAbiotic stress responsesen
dc.subjectABAen
dc.titleHerboxidiene triggers splicing repression and abiotic stress responses in plantsen
dc.typeArticleen
dc.contributor.departmentLaboratory for Genome Engineering, Division of Biological Sciences, 4700 King Abdullah University of Science and Technologyen
dc.identifier.journalBMC Genomicsen
dc.eprint.versionPublisher's Version/PDFen
kaust.authorAlshareef, Saharen
kaust.authorLing, Yuen
kaust.authorButt, Haroonen
kaust.authorMariappan, Kiruthiga G.en
kaust.authorBenhamed, Moussaen
kaust.authorMahfouz, Magdy M.en
dc.relation.isSupplementedByAlShareef, S., Ling, Y., Butt, H., Kiruthiga Mariappan, Benhamed, M., & Magdy Mahfouz. (2017). Herboxidiene triggers splicing repression and abiotic stress responses in plants. Figshare. https://doi.org/10.6084/m9.figshare.c.3727597en
dc.relation.isSupplementedByDOI:10.6084/m9.figshare.c.3727597en
dc.relation.isSupplementedByHANDLE:http://hdl.handle.net/10754/624149en
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