Bioactive Potential of Marine Macroalgae from the Central Red Sea (Saudi Arabia) Assessed by High-Throughput Imaging-Based Phenotypic Profiling

Handle URI:
http://hdl.handle.net/10754/623072
Title:
Bioactive Potential of Marine Macroalgae from the Central Red Sea (Saudi Arabia) Assessed by High-Throughput Imaging-Based Phenotypic Profiling
Authors:
Kremb, Stephan Georg; Müller, Constanze; Schmitt-Kopplin, Philippe; Voolstra, Christian R. ( 0000-0003-4555-3795 )
Abstract:
Marine algae represent an important source of novel natural products. While their bioactive potential has been studied to some extent, limited information is available on marine algae from the Red Sea. This study aimed at the broad discovery of new bioactivities from a collection of twelve macroalgal species from the Central Red Sea. We used imaging-based High-Content Screening (HCS) with a diverse spectrum of cellular markers for detailed cytological profiling of fractionated algal extracts. The cytological profiles for 3 out of 60 algal fractions clustered closely to reference inhibitors and showed strong inhibitory activities on the HIV-1 reverse transcriptase in a single-enzyme biochemical assay, validating the suggested biological target. Subsequent chemical profiling of the active fractions of two brown algal species by ultra-high resolution mass spectrometry (FT-ICR-MS) revealed possible candidate molecules. A database query of these molecules led us to groups of compounds with structural similarities, which are suggested to be responsible for the observed activity. Our work demonstrates the versatility and power of cytological profiling for the bioprospecting of unknown biological resources and highlights Red Sea algae as a source of bioactives that may serve as a starting point for further studies.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Red Sea Research Center (RSRC)
Citation:
Kremb S, Müller C, Schmitt-Kopplin P, Voolstra C (2017) Bioactive Potential of Marine Macroalgae from the Central Red Sea (Saudi Arabia) Assessed by High-Throughput Imaging-Based Phenotypic Profiling. Marine Drugs 15: 80. Available: http://dx.doi.org/10.3390/md15030080.
Publisher:
MDPI AG
Journal:
Marine Drugs
Issue Date:
20-Mar-2017
DOI:
10.3390/md15030080
Type:
Article
ISSN:
1660-3397
Sponsors:
The following reagents were obtained through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH: HeLa cells, D4T, DDI, Emtricitabine, beta-D-N4hydroxycytidine, and ddC. Research reported in this publication was supported by the King Abdullah University of Science and Technology (KAUST). We thank Horst Wolff (Carl Zeiss Microscopy, Germany) and Birgit Kraus (University of Regensburg, Germany) for assistance in the setup of the screening panels and imaging software. We would also like to thank Sami Alqarawi and the KAUST Bioscience Core lab for assistance.
Additional Links:
http://www.mdpi.com/1660-3397/15/3/80
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorKremb, Stephan Georgen
dc.contributor.authorMüller, Constanzeen
dc.contributor.authorSchmitt-Kopplin, Philippeen
dc.contributor.authorVoolstra, Christian R.en
dc.date.accessioned2017-04-10T07:49:49Z-
dc.date.available2017-04-10T07:49:49Z-
dc.date.issued2017-03-20en
dc.identifier.citationKremb S, Müller C, Schmitt-Kopplin P, Voolstra C (2017) Bioactive Potential of Marine Macroalgae from the Central Red Sea (Saudi Arabia) Assessed by High-Throughput Imaging-Based Phenotypic Profiling. Marine Drugs 15: 80. Available: http://dx.doi.org/10.3390/md15030080.en
dc.identifier.issn1660-3397en
dc.identifier.doi10.3390/md15030080en
dc.identifier.urihttp://hdl.handle.net/10754/623072-
dc.description.abstractMarine algae represent an important source of novel natural products. While their bioactive potential has been studied to some extent, limited information is available on marine algae from the Red Sea. This study aimed at the broad discovery of new bioactivities from a collection of twelve macroalgal species from the Central Red Sea. We used imaging-based High-Content Screening (HCS) with a diverse spectrum of cellular markers for detailed cytological profiling of fractionated algal extracts. The cytological profiles for 3 out of 60 algal fractions clustered closely to reference inhibitors and showed strong inhibitory activities on the HIV-1 reverse transcriptase in a single-enzyme biochemical assay, validating the suggested biological target. Subsequent chemical profiling of the active fractions of two brown algal species by ultra-high resolution mass spectrometry (FT-ICR-MS) revealed possible candidate molecules. A database query of these molecules led us to groups of compounds with structural similarities, which are suggested to be responsible for the observed activity. Our work demonstrates the versatility and power of cytological profiling for the bioprospecting of unknown biological resources and highlights Red Sea algae as a source of bioactives that may serve as a starting point for further studies.en
dc.description.sponsorshipThe following reagents were obtained through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH: HeLa cells, D4T, DDI, Emtricitabine, beta-D-N4hydroxycytidine, and ddC. Research reported in this publication was supported by the King Abdullah University of Science and Technology (KAUST). We thank Horst Wolff (Carl Zeiss Microscopy, Germany) and Birgit Kraus (University of Regensburg, Germany) for assistance in the setup of the screening panels and imaging software. We would also like to thank Sami Alqarawi and the KAUST Bioscience Core lab for assistance.en
dc.publisherMDPI AGen
dc.relation.urlhttp://www.mdpi.com/1660-3397/15/3/80en
dc.rightsThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectbioprospectingen
dc.subjectnatural productsen
dc.subjectalgaeen
dc.subjectcytological profilingen
dc.subjectHigh-Content Screeningen
dc.subjectHIVen
dc.titleBioactive Potential of Marine Macroalgae from the Central Red Sea (Saudi Arabia) Assessed by High-Throughput Imaging-Based Phenotypic Profilingen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalMarine Drugsen
dc.contributor.institutionResearch Unit Analytical Biogeochemistry, Helmholtz Zentrum München, Ingolstaedter Landstrasse 1, D-85764 Neuherberg, Germanyen
dc.contributor.institutionChair of Analytical Food Chemistry, Technische Universität München (TUM), 85354 Freising-Weihenstephan, Germanyen
kaust.authorKremb, Stephan Georgen
kaust.authorVoolstra, Christian R.en
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