Strigolactone biosynthesis is evolutionarily conserved, regulated by phosphate starvation and contributes to resistance against phytopathogenic fungi in a moss, Physcomitrella patens

Handle URI:
http://hdl.handle.net/10754/623062
Title:
Strigolactone biosynthesis is evolutionarily conserved, regulated by phosphate starvation and contributes to resistance against phytopathogenic fungi in a moss, Physcomitrella patens
Authors:
Decker, Eva L.; Alder, Adrian; Hunn, Stefan; Ferguson, Jenny; Lehtonen, Mikko T.; Scheler, Bjoern; Kerres, Klaus L.; Wiedemann, Gertrud; Safavi-Rizi, Vajiheh; Nordzieke, Steffen; Balakrishna, Aparna; Baz, Lina Abdulkareem Ali; Avalos, Javier; Valkonen, Jari P. T.; Reski, Ralf ( 0000-0002-5496-6711 ) ; Al-Babili, Salim ( 0000-0003-4823-2882 )
Abstract:
In seed plants, strigolactones (SLs) regulate architecture and induce mycorrhizal symbiosis in response to environmental cues. SLs are formed by combined activity of the carotenoid cleavage dioxygenases (CCDs) 7 and 8 from 9-cis-β-carotene, leading to carlactone that is converted by cytochromes P450 (clade 711; MAX1 in Arabidopsis) into various SLs. As Physcomitrella patens possesses CCD7 and CCD8 homologs but lacks MAX1, we investigated if PpCCD7 together with PpCCD8 form carlactone and how deletion of these enzymes influences growth and interactions with the environment. We investigated the enzymatic activity of PpCCD7 and PpCCD8 in vitro, identified the formed products by high performance liquid chromatography (HPLC) and LC-MS, and generated and analysed ΔCCD7 and ΔCCD8 mutants. We defined enzymatic activity of PpCCD7 as a stereospecific 9-cis-CCD and PpCCD8 as a carlactone synthase. ΔCCD7 and ΔCCD8 lines showed enhanced caulonema growth, which was revertible by adding the SL analogue GR24 or carlactone. Wild-type (WT) exudates induced seed germination in Orobanche ramosa. This activity was increased upon phosphate starvation and abolished in exudates of both mutants. Furthermore, both mutants showed increased susceptibility to phytopathogenic fungi. Our study reveals the deep evolutionary conservation of SL biosynthesis, SL function, and its regulation by biotic and abiotic cues.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Plant Science Program
Citation:
Decker EL, Alder A, Hunn S, Ferguson J, Lehtonen MT, et al. (2017) Strigolactone biosynthesis is evolutionarily conserved, regulated by phosphate starvation and contributes to resistance against phytopathogenic fungi in a moss, Physcomitrella patens . New Phytologist. Available: http://dx.doi.org/10.1111/nph.14506.
Publisher:
Wiley-Blackwell
Journal:
New Phytologist
Issue Date:
6-Mar-2017
DOI:
10.1111/nph.14506
Type:
Article
ISSN:
0028-646X
Sponsors:
We thank Harro Bouwmeester and Carolien Ruyter-Spira for valuable discussions, Agnes Novakovic for supporting the generation and analysis of DCCD lines, and Anne Katrin Prowse for editing the language of the manuscript. This work was supported by the Deutsche Forschungsgemeinschaft (DFG; grant AL892/1- 4 and the Graduiertenkolleg 1305 ‘Plant Signal Systems’), King Abdullah University of Science and Technology (KAUST; baseline funding of S.A-B.), the Excellence Initiative of the German Federal and State Governments (grant no. EXC 294 BIOSS to R.R.), and The Academy of Finland (grant 1253126 to J.P.T.V.).
Additional Links:
http://onlinelibrary.wiley.com/doi/10.1111/nph.14506/abstract
Appears in Collections:
Articles; Plant Science Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorDecker, Eva L.en
dc.contributor.authorAlder, Adrianen
dc.contributor.authorHunn, Stefanen
dc.contributor.authorFerguson, Jennyen
dc.contributor.authorLehtonen, Mikko T.en
dc.contributor.authorScheler, Bjoernen
dc.contributor.authorKerres, Klaus L.en
dc.contributor.authorWiedemann, Gertruden
dc.contributor.authorSafavi-Rizi, Vajihehen
dc.contributor.authorNordzieke, Steffenen
dc.contributor.authorBalakrishna, Aparnaen
dc.contributor.authorBaz, Lina Abdulkareem Alien
dc.contributor.authorAvalos, Javieren
dc.contributor.authorValkonen, Jari P. T.en
dc.contributor.authorReski, Ralfen
dc.contributor.authorAl-Babili, Salimen
dc.date.accessioned2017-03-23T11:04:33Z-
dc.date.available2017-03-23T11:04:33Z-
dc.date.issued2017-03-06en
dc.identifier.citationDecker EL, Alder A, Hunn S, Ferguson J, Lehtonen MT, et al. (2017) Strigolactone biosynthesis is evolutionarily conserved, regulated by phosphate starvation and contributes to resistance against phytopathogenic fungi in a moss, Physcomitrella patens . New Phytologist. Available: http://dx.doi.org/10.1111/nph.14506.en
dc.identifier.issn0028-646Xen
dc.identifier.doi10.1111/nph.14506en
dc.identifier.urihttp://hdl.handle.net/10754/623062-
dc.description.abstractIn seed plants, strigolactones (SLs) regulate architecture and induce mycorrhizal symbiosis in response to environmental cues. SLs are formed by combined activity of the carotenoid cleavage dioxygenases (CCDs) 7 and 8 from 9-cis-β-carotene, leading to carlactone that is converted by cytochromes P450 (clade 711; MAX1 in Arabidopsis) into various SLs. As Physcomitrella patens possesses CCD7 and CCD8 homologs but lacks MAX1, we investigated if PpCCD7 together with PpCCD8 form carlactone and how deletion of these enzymes influences growth and interactions with the environment. We investigated the enzymatic activity of PpCCD7 and PpCCD8 in vitro, identified the formed products by high performance liquid chromatography (HPLC) and LC-MS, and generated and analysed ΔCCD7 and ΔCCD8 mutants. We defined enzymatic activity of PpCCD7 as a stereospecific 9-cis-CCD and PpCCD8 as a carlactone synthase. ΔCCD7 and ΔCCD8 lines showed enhanced caulonema growth, which was revertible by adding the SL analogue GR24 or carlactone. Wild-type (WT) exudates induced seed germination in Orobanche ramosa. This activity was increased upon phosphate starvation and abolished in exudates of both mutants. Furthermore, both mutants showed increased susceptibility to phytopathogenic fungi. Our study reveals the deep evolutionary conservation of SL biosynthesis, SL function, and its regulation by biotic and abiotic cues.en
dc.description.sponsorshipWe thank Harro Bouwmeester and Carolien Ruyter-Spira for valuable discussions, Agnes Novakovic for supporting the generation and analysis of DCCD lines, and Anne Katrin Prowse for editing the language of the manuscript. This work was supported by the Deutsche Forschungsgemeinschaft (DFG; grant AL892/1- 4 and the Graduiertenkolleg 1305 ‘Plant Signal Systems’), King Abdullah University of Science and Technology (KAUST; baseline funding of S.A-B.), the Excellence Initiative of the German Federal and State Governments (grant no. EXC 294 BIOSS to R.R.), and The Academy of Finland (grant 1253126 to J.P.T.V.).en
dc.publisherWiley-Blackwellen
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1111/nph.14506/abstracten
dc.rightsThis is the peer reviewed version of the following article: Strigolactone biosynthesis is evolutionarily conserved, regulated by phosphate starvation and contributes to resistance against phytopathogenic fungi in a moss, Physcomitrella patens, which has been published in final form at http://doi.org/10.1111/nph.14506. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.en
dc.subjectCarotenoidsen
dc.subjectPhosphate starvationen
dc.subjectSclerotinia sclerotiorumen
dc.subjectPhyscomitrella patensen
dc.subjectStrigolactonesen
dc.subjectOrobanche Ramosaen
dc.subjectCarotenoid Cleavage Dioxygenaseen
dc.subjectCarlactoneen
dc.titleStrigolactone biosynthesis is evolutionarily conserved, regulated by phosphate starvation and contributes to resistance against phytopathogenic fungi in a moss, Physcomitrella patensen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentPlant Science Programen
dc.identifier.journalNew Phytologisten
dc.eprint.versionPost-printen
dc.contributor.institutionPlant Biotechnology; Faculty of Biology; University of Freiburg; Schaenzlestr. 1 Freiburg 79104 Germanyen
dc.contributor.institutionCell Biology; Faculty of Biology; University of Freiburg; Schaenzlestr. 1 Freiburg 79104 Germanyen
dc.contributor.institutionDepartment of Agricultural Sciences; University of Helsinki; Latokartanonkaari 7 Helsinki FIN-00014 Finlanden
dc.contributor.institutionDepartamento de Genética; Facultad de Biología; Universidad de Sevilla; Seville E-41080 Spainen
dc.contributor.institutionFRIAS - Freiburg Institute for Advanced Studies; University of Freiburg; Freiburg 79104 Germanyen
dc.contributor.institutionBIOSS - Centre for Biological Signalling Studies; University of Freiburg; Freiburg 79104 Germanyen
kaust.authorBalakrishna, Aparnaen
kaust.authorBaz, Lina Abdulkareem Alien
kaust.authorAl-Babili, Salimen
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