High-resolution phenotypic profiling of natural products-induced effects on the single-cell level

Handle URI:
http://hdl.handle.net/10754/623036
Title:
High-resolution phenotypic profiling of natural products-induced effects on the single-cell level
Authors:
Kremb, Stephan Georg; Voolstra, Christian R. ( 0000-0003-4555-3795 )
Abstract:
Natural products (NPs) are highly evolved molecules making them a valuable resource for new therapeutics. Here we demonstrate the usefulness of broad-spectrum phenotypic profiling of NP-induced perturbations on single cells with imaging-based High-Content Screening to inform on physiology, mechanisms-of-actions, and multi-level toxicity. Our technology platform aims at broad applicability using a comprehensive marker panel with standardized settings streamlined towards an easy implementation in laboratories dedicated to natural products research.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Red Sea Research Center (RSRC)
Citation:
Kremb S, Voolstra CR (2017) High-resolution phenotypic profiling of natural products-induced effects on the single-cell level. Scientific Reports 7: 44472. Available: http://dx.doi.org/10.1038/srep44472.
Publisher:
Springer Nature
Journal:
Scientific Reports
Issue Date:
15-Mar-2017
DOI:
10.1038/srep44472
Type:
Article
ISSN:
2045-2322
Sponsors:
We thank the Bioscience core lab for supporting cell culture work and providing the Cellomics ArrayScan HCS instrument. Research in this publication was supported by baseline funds to CRV from King Abdullah University of Science and Technology (KAUST).
Additional Links:
http://www.nature.com/articles/srep44472
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorKremb, Stephan Georgen
dc.contributor.authorVoolstra, Christian R.en
dc.date.accessioned2017-03-20T08:46:09Z-
dc.date.available2017-03-20T08:46:09Z-
dc.date.issued2017-03-15en
dc.identifier.citationKremb S, Voolstra CR (2017) High-resolution phenotypic profiling of natural products-induced effects on the single-cell level. Scientific Reports 7: 44472. Available: http://dx.doi.org/10.1038/srep44472.en
dc.identifier.issn2045-2322en
dc.identifier.doi10.1038/srep44472en
dc.identifier.urihttp://hdl.handle.net/10754/623036-
dc.description.abstractNatural products (NPs) are highly evolved molecules making them a valuable resource for new therapeutics. Here we demonstrate the usefulness of broad-spectrum phenotypic profiling of NP-induced perturbations on single cells with imaging-based High-Content Screening to inform on physiology, mechanisms-of-actions, and multi-level toxicity. Our technology platform aims at broad applicability using a comprehensive marker panel with standardized settings streamlined towards an easy implementation in laboratories dedicated to natural products research.en
dc.description.sponsorshipWe thank the Bioscience core lab for supporting cell culture work and providing the Cellomics ArrayScan HCS instrument. Research in this publication was supported by baseline funds to CRV from King Abdullah University of Science and Technology (KAUST).en
dc.publisherSpringer Natureen
dc.relation.urlhttp://www.nature.com/articles/srep44472en
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleHigh-resolution phenotypic profiling of natural products-induced effects on the single-cell levelen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalScientific Reportsen
dc.eprint.versionPublisher's Version/PDFen
kaust.authorKremb, Stephan Georgen
kaust.authorVoolstra, Christian R.en
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.