Phosphorylation of the dimeric cytoplasmic domain of the phytosulfokine receptor, PSKR1

Handle URI:
http://hdl.handle.net/10754/622902
Title:
Phosphorylation of the dimeric cytoplasmic domain of the phytosulfokine receptor, PSKR1
Authors:
Muleya, V.; Marondedze, Claudius ( 0000-0002-2113-904X ) ; Wheeler, J. I.; Thomas, Ludivine; Mok, Y.-F.; Griffin, M. D. W.; Manallack, D. T.; Kwezi, L.; Lilley, K. S.; Gehring, Christoph A. ( 0000-0003-4355-4591 ) ; Irving, H. R.
Abstract:
Phytosulfokines (PSKs) are plant peptide hormones that co-regulate plant growth, differentiation and defense responses. PSKs signal through a plasma membrane localized leucine-rich repeat receptor-like kinase (phytosulfokine receptor 1, PSKR1) that also contains a functional cytosolic guanylate cyclase with its cyclase catalytic center embedded within the kinase domain. To functionally characterize this novel type of overlapping dual catalytic function, we investigated the phosphorylation of PSKR1 in vitro Tandem mass spectrometry of the cytoplasmic domain of PSKR1 (PSKR1cd) revealed at least 11 phosphorylation sites (8 serines, 2 threonines and 1 tyrosine) within the PSKR1cd. Phosphomimetic mutations of three serine residues (Ser686, Ser696 and Ser698) in tandem at the juxta-membrane position resulted in enhanced kinase activity in the on-mutant that was suppressed in the off-mutant, but both mutations reduced guanylate cyclase activity. Both the on and off phosphomimetic mutations of the phosphotyrosine (Tyr888) residue in the activation loop suppressed kinase activity, while neither mutation affected guanylate cyclase activity. Size exclusion and analytical ultracentrifugation analysis of the PSKR1cd suggest that it is reversibly dimeric in solution, which was further confirmed by biflourescence complementation. Taken together, these data suggest that in this novel type of receptor domain architecture, specific phosphorylation and dimerization are possibly essential mechanisms for ligand-mediated catalysis and signaling.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Muleya V, Marondedze C, Wheeler JI, Thomas L, Mok Y-F, et al. (2016) Phosphorylation of the dimeric cytoplasmic domain of the phytosulfokine receptor, PSKR1. Biochemical Journal 473: 3081–3098. Available: http://dx.doi.org/10.1042/bcj20160593.
Publisher:
Portland Press Ltd.
Journal:
Biochemical Journal
Issue Date:
4-Aug-2016
DOI:
10.1042/bcj20160593
Type:
Article
ISSN:
0264-6021; 1470-8728
Sponsors:
Support from the Australian Research Council's Discovery funding scheme [project numbers DP0878194 and DP110104164] and the National Research Foundation South Africa [grant numbers 78843; IRF2009021800047] is gratefully acknowledged. V.M. was supported by a scholarship from the Monash Institute of Pharmaceutical Sciences. M.D.W.G. is the recipient of an Australian Research Council Post-Doctoral Fellowship [DP110103528] and the CR Roper Fellowship. C.G. was supported by King Abdullah University of Science and Technology [KAUST; BAS/1/1013-01-01]. The mass spectrometry was performed using equipment purchased via a Wellcome Trust grant [099135/Z/12/Z].
Additional Links:
http://www.biochemj.org/content/473/19/3081
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorMuleya, V.en
dc.contributor.authorMarondedze, Claudiusen
dc.contributor.authorWheeler, J. I.en
dc.contributor.authorThomas, Ludivineen
dc.contributor.authorMok, Y.-F.en
dc.contributor.authorGriffin, M. D. W.en
dc.contributor.authorManallack, D. T.en
dc.contributor.authorKwezi, L.en
dc.contributor.authorLilley, K. S.en
dc.contributor.authorGehring, Christoph A.en
dc.contributor.authorIrving, H. R.en
dc.date.accessioned2017-02-15T08:32:15Z-
dc.date.available2017-02-15T08:32:15Z-
dc.date.issued2016-08-04en
dc.identifier.citationMuleya V, Marondedze C, Wheeler JI, Thomas L, Mok Y-F, et al. (2016) Phosphorylation of the dimeric cytoplasmic domain of the phytosulfokine receptor, PSKR1. Biochemical Journal 473: 3081–3098. Available: http://dx.doi.org/10.1042/bcj20160593.en
dc.identifier.issn0264-6021en
dc.identifier.issn1470-8728en
dc.identifier.doi10.1042/bcj20160593en
dc.identifier.urihttp://hdl.handle.net/10754/622902-
dc.description.abstractPhytosulfokines (PSKs) are plant peptide hormones that co-regulate plant growth, differentiation and defense responses. PSKs signal through a plasma membrane localized leucine-rich repeat receptor-like kinase (phytosulfokine receptor 1, PSKR1) that also contains a functional cytosolic guanylate cyclase with its cyclase catalytic center embedded within the kinase domain. To functionally characterize this novel type of overlapping dual catalytic function, we investigated the phosphorylation of PSKR1 in vitro Tandem mass spectrometry of the cytoplasmic domain of PSKR1 (PSKR1cd) revealed at least 11 phosphorylation sites (8 serines, 2 threonines and 1 tyrosine) within the PSKR1cd. Phosphomimetic mutations of three serine residues (Ser686, Ser696 and Ser698) in tandem at the juxta-membrane position resulted in enhanced kinase activity in the on-mutant that was suppressed in the off-mutant, but both mutations reduced guanylate cyclase activity. Both the on and off phosphomimetic mutations of the phosphotyrosine (Tyr888) residue in the activation loop suppressed kinase activity, while neither mutation affected guanylate cyclase activity. Size exclusion and analytical ultracentrifugation analysis of the PSKR1cd suggest that it is reversibly dimeric in solution, which was further confirmed by biflourescence complementation. Taken together, these data suggest that in this novel type of receptor domain architecture, specific phosphorylation and dimerization are possibly essential mechanisms for ligand-mediated catalysis and signaling.en
dc.description.sponsorshipSupport from the Australian Research Council's Discovery funding scheme [project numbers DP0878194 and DP110104164] and the National Research Foundation South Africa [grant numbers 78843; IRF2009021800047] is gratefully acknowledged. V.M. was supported by a scholarship from the Monash Institute of Pharmaceutical Sciences. M.D.W.G. is the recipient of an Australian Research Council Post-Doctoral Fellowship [DP110103528] and the CR Roper Fellowship. C.G. was supported by King Abdullah University of Science and Technology [KAUST; BAS/1/1013-01-01]. The mass spectrometry was performed using equipment purchased via a Wellcome Trust grant [099135/Z/12/Z].en
dc.publisherPortland Press Ltd.en
dc.relation.urlhttp://www.biochemj.org/content/473/19/3081en
dc.subjectDimerizationen
dc.subjectGuanylate cyclaseen
dc.subjectDual-specificity Kinaseen
dc.subjectLeucine-rich Receptor-like Kinaseen
dc.titlePhosphorylation of the dimeric cytoplasmic domain of the phytosulfokine receptor, PSKR1en
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalBiochemical Journalen
dc.contributor.institutionMonash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, VIC 3052, Australia.en
dc.contributor.institutionCambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, Cambridge, U.K.en
dc.contributor.institutionDepartment of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, 30 Flemington Rd, Parkville, VIC 3010, Australia.en
kaust.authorMarondedze, Claudiusen
kaust.authorThomas, Ludivineen
kaust.authorGehring, Christoph A.en
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