The transition from a maternal to external nitrogen source in maize seedlings

Handle URI:
http://hdl.handle.net/10754/622875
Title:
The transition from a maternal to external nitrogen source in maize seedlings
Authors:
Sabermanesh, Kasra; Holtham, Luke R.; George, Jessey; Roessner, Ute; Boughton, Berin A.; Heuer, Sigrid; Tester, Mark A. ( 0000-0002-5085-8801 ) ; Plett, Darren C.; Garnett, Trevor P.
Abstract:
Maximising NO3 - uptake during seedling development is important as it has a major influence on plant growth and yield. However, little is known about the processes leading to, and involved in, the initiation of root NO3 - uptake capacity in developing seedlings. This study examines the physiological processes involved in root NO3 - uptake and metabolism, to gain an understanding of how the NO3 - uptake system responds to meet demand as maize seedlings transition from seed N use to external N capture. The concentrations of seed-derived free amino acids within root and shoot tissues are initially high, but decrease rapidly until stabilising eight days after imbibition (DAI). Similarly, shoot N% decreases, but does not stabilise until 12-13 DAI. Following the decrease in free amino acid concentrations, root NO3 - uptake capacity increases until shoot N% stabilises. The increase in root NO3 uptake capacity corresponds with a rapid rise in transcript levels of putative NO3 - transporters, ZmNRT2.1 and ZmNRT2.2. The processes underlying the increase in root NO3 - uptake capacity to meet N demand provide an insight into the processes controlling N uptake.
KAUST Department:
Center for Desert Agriculture
Citation:
Sabermanesh K, Holtham LR, George J, Roessner U, Boughton BA, et al. (2017) The transition from a maternal to external nitrogen source in maize seedlings. Journal of Integrative Plant Biology. Available: http://dx.doi.org/10.1111/jipb.12525.
Publisher:
Wiley-Blackwell
Journal:
Journal of Integrative Plant Biology
Issue Date:
7-Feb-2017
DOI:
10.1111/jipb.12525
Type:
Article
ISSN:
1672-9072
Sponsors:
We thank Hanne Thompson, Yuan Li, Nenah Mackenzie, Priyanka Reddy and Chia Ng for the technical assistance provided for this study. We also thank Metabolomics Australia, School of BioSciences, The University of Melbourne, for sample preparation and amino acid analysis. Ute Roessner and Berin Boughton are also grateful to Victorian Node of Metabolomics Australia, which is funded through Bioplatforms Australia Pty Ltd, a National Collaborative Research Infrastructure Strategy, 5.1 Biomolecular Platforms and informatics investment, and co-investment from the Victorian State Government and The University of Melbourne. This project was supported by the Australian Centre for Plant Functional Genomics, the Australian Research Council (LP130101055), DuPont Pioneer and the Grains Research and Development Corporation (GRS10437).
Additional Links:
http://onlinelibrary.wiley.com/doi/10.1111/jipb.12525/abstract
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorSabermanesh, Kasraen
dc.contributor.authorHoltham, Luke R.en
dc.contributor.authorGeorge, Jesseyen
dc.contributor.authorRoessner, Uteen
dc.contributor.authorBoughton, Berin A.en
dc.contributor.authorHeuer, Sigriden
dc.contributor.authorTester, Mark A.en
dc.contributor.authorPlett, Darren C.en
dc.contributor.authorGarnett, Trevor P.en
dc.date.accessioned2017-02-12T13:38:50Z-
dc.date.available2017-02-12T13:38:50Z-
dc.date.issued2017-02-07en
dc.identifier.citationSabermanesh K, Holtham LR, George J, Roessner U, Boughton BA, et al. (2017) The transition from a maternal to external nitrogen source in maize seedlings. Journal of Integrative Plant Biology. Available: http://dx.doi.org/10.1111/jipb.12525.en
dc.identifier.issn1672-9072en
dc.identifier.doi10.1111/jipb.12525en
dc.identifier.urihttp://hdl.handle.net/10754/622875-
dc.description.abstractMaximising NO3 - uptake during seedling development is important as it has a major influence on plant growth and yield. However, little is known about the processes leading to, and involved in, the initiation of root NO3 - uptake capacity in developing seedlings. This study examines the physiological processes involved in root NO3 - uptake and metabolism, to gain an understanding of how the NO3 - uptake system responds to meet demand as maize seedlings transition from seed N use to external N capture. The concentrations of seed-derived free amino acids within root and shoot tissues are initially high, but decrease rapidly until stabilising eight days after imbibition (DAI). Similarly, shoot N% decreases, but does not stabilise until 12-13 DAI. Following the decrease in free amino acid concentrations, root NO3 - uptake capacity increases until shoot N% stabilises. The increase in root NO3 uptake capacity corresponds with a rapid rise in transcript levels of putative NO3 - transporters, ZmNRT2.1 and ZmNRT2.2. The processes underlying the increase in root NO3 - uptake capacity to meet N demand provide an insight into the processes controlling N uptake.en
dc.description.sponsorshipWe thank Hanne Thompson, Yuan Li, Nenah Mackenzie, Priyanka Reddy and Chia Ng for the technical assistance provided for this study. We also thank Metabolomics Australia, School of BioSciences, The University of Melbourne, for sample preparation and amino acid analysis. Ute Roessner and Berin Boughton are also grateful to Victorian Node of Metabolomics Australia, which is funded through Bioplatforms Australia Pty Ltd, a National Collaborative Research Infrastructure Strategy, 5.1 Biomolecular Platforms and informatics investment, and co-investment from the Victorian State Government and The University of Melbourne. This project was supported by the Australian Centre for Plant Functional Genomics, the Australian Research Council (LP130101055), DuPont Pioneer and the Grains Research and Development Corporation (GRS10437).en
dc.publisherWiley-Blackwellen
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1111/jipb.12525/abstracten
dc.rightsThis is the peer reviewed version of the following article: The transition from a maternal to external nitrogen source in maize seedlings, which has been published in final form at http://doi.org/10.1111/jipb.12525. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.en
dc.subjectZea maysen
dc.subjectAmino acidsen
dc.subjectSeeden
dc.subjectSeedlingen
dc.subjectNitrogen use efficiencyen
dc.subjectRootsen
dc.subjectNitrate Uptakeen
dc.subjectNrten
dc.subjectNpfen
dc.titleThe transition from a maternal to external nitrogen source in maize seedlingsen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalJournal of Integrative Plant Biologyen
dc.eprint.versionPost-printen
dc.contributor.institutionAustralian Centre for Plant Functional Genomics; Waite Research Institute, University of Adelaide; Adelaide, SA 5064, Australiaen
dc.contributor.institutionSchool of Agriculture, Food and Wine; Waite Research Institute, University of Adelaide; Adelaide, SA 5064 Australiaen
dc.contributor.institutionAustralian Centre for Plant Functional Genomics; School of BioSciences, University of Melbourne; Parkville, Vic. 3010 Australiaen
dc.contributor.institutionMetabolomics Australia; School of BioSciences, University of Melbourne; Vic. 3010 Australiaen
kaust.authorTester, Mark A.en
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