Shedding light on the role of photosynthesis in pathogen colonization and host defense

Handle URI:
http://hdl.handle.net/10754/622832
Title:
Shedding light on the role of photosynthesis in pathogen colonization and host defense
Authors:
Garavaglia, Betiana S.; Thomas, Ludivine; Gottig, Natalia; Zimaro, Tamara; Garofalo, Cecilia G.; Gehring, Christoph A. ( 0000-0003-4355-4591 ) ; Ottado, Jorgelina
Abstract:
The role of photosynthesis in plant defense is a fundamental question awaiting further molecular and physiological elucidation. To this end we investigated host responses to infection with the bacterial pathogen Xanthomonas axonopodis pv. citri, the pathogen responsible for citrus canker. This pathogen encodes a plant-like natriuretic peptide (XacPNP) that is expressed specifically during the infection process and prevents deterioration of the physiological condition of the infected tissue. Proteomic assays of citrus leaves infected with a XacPNP deletion mutant (DeltaXacPNP) resulted in a major reduction in photosynthetic proteins such as Rubisco, Rubisco activase and ATP synthase as a compared with infection with wild type bacteria. In contrast, infiltration of citrus leaves with recombinant XacPNP caused an increase in these host proteins and a concomitant increase in photosynthetic efficiency as measured by chlorophyll fluorescence assays. Reversion of the reduction in photosynthetic efficiency in citrus leaves infected with DeltaXacPNP was achieved by the application of XacPNP or Citrus sinensis PNP lending support to a case of molecular mimicry. Finally, given that DeltaXacPNP infection is less successful than infection with the wild type, it appears that reducing photosynthesis is an effective plant defense mechanism against biotrophic pathogens.
KAUST Department:
Computational Bioscience Research Center (CBRC)
Citation:
Garavaglia BS, Thomas L, Gottig N, Zimaro T, Garofalo CG, et al. (2010) Shedding light on the role of photosynthesis in pathogen colonization and host defense. Communicative & Integrative Biology 3: 382–384. Available: http://dx.doi.org/10.4161/cib.3.4.12029.
Publisher:
Informa UK Limited
Journal:
Communicative & Integrative Biology
Issue Date:
1-Sep-2010
DOI:
10.4161/cib.3.4.12029
Type:
Article
ISSN:
1942-0889
Additional Links:
http://www.tandfonline.com/action/showCopyRight?scroll=top&doi=10.4161%2Fcib.3.4.12029
Appears in Collections:
Articles; Computational Bioscience Research Center (CBRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorGaravaglia, Betiana S.en
dc.contributor.authorThomas, Ludivineen
dc.contributor.authorGottig, Nataliaen
dc.contributor.authorZimaro, Tamaraen
dc.contributor.authorGarofalo, Cecilia G.en
dc.contributor.authorGehring, Christoph A.en
dc.contributor.authorOttado, Jorgelinaen
dc.date.accessioned2017-02-05T13:53:45Z-
dc.date.available2017-02-05T13:53:45Z-
dc.date.issued2010-09-01en
dc.identifier.citationGaravaglia BS, Thomas L, Gottig N, Zimaro T, Garofalo CG, et al. (2010) Shedding light on the role of photosynthesis in pathogen colonization and host defense. Communicative & Integrative Biology 3: 382–384. Available: http://dx.doi.org/10.4161/cib.3.4.12029.en
dc.identifier.issn1942-0889en
dc.identifier.doi10.4161/cib.3.4.12029en
dc.identifier.urihttp://hdl.handle.net/10754/622832-
dc.description.abstractThe role of photosynthesis in plant defense is a fundamental question awaiting further molecular and physiological elucidation. To this end we investigated host responses to infection with the bacterial pathogen Xanthomonas axonopodis pv. citri, the pathogen responsible for citrus canker. This pathogen encodes a plant-like natriuretic peptide (XacPNP) that is expressed specifically during the infection process and prevents deterioration of the physiological condition of the infected tissue. Proteomic assays of citrus leaves infected with a XacPNP deletion mutant (DeltaXacPNP) resulted in a major reduction in photosynthetic proteins such as Rubisco, Rubisco activase and ATP synthase as a compared with infection with wild type bacteria. In contrast, infiltration of citrus leaves with recombinant XacPNP caused an increase in these host proteins and a concomitant increase in photosynthetic efficiency as measured by chlorophyll fluorescence assays. Reversion of the reduction in photosynthetic efficiency in citrus leaves infected with DeltaXacPNP was achieved by the application of XacPNP or Citrus sinensis PNP lending support to a case of molecular mimicry. Finally, given that DeltaXacPNP infection is less successful than infection with the wild type, it appears that reducing photosynthesis is an effective plant defense mechanism against biotrophic pathogens.en
dc.publisherInforma UK Limiteden
dc.relation.urlhttp://www.tandfonline.com/action/showCopyRight?scroll=top&doi=10.4161%2Fcib.3.4.12029en
dc.rightsThis is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.en
dc.subjectPhotosynthetic efficiencyen
dc.subjectXanthomonasen
dc.subjectPlant-pathogen Interactionen
dc.subjectPlant Natriuretic Peptideen
dc.subjectCitrus Cankeren
dc.titleShedding light on the role of photosynthesis in pathogen colonization and host defenseen
dc.typeArticleen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journalCommunicative & Integrative Biologyen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionMolecular Biology Division; Instituto de Biología Molecular y Celular de Rosario; Consejo Nacional de Investigaciones Científicas y Técnicas; Facultad de Ciencias Bioquímicas y Farmacéuticas; Universidad Nacional de Rosario; Rosario, Argentinaen
dc.contributor.institutionConsejo de Investigaciones de la Universidad Nacional de Rosario; Rosario, Argentinaen
dc.contributor.institutionDepartment of Biotechnology; University of the western Cape; Bellville, South Africaen
kaust.authorGehring, Christoph A.en
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