Engineering Plant Immunity: Using CRISPR/Cas9 to Generate Virus Resistance

Handle URI:
http://hdl.handle.net/10754/622815
Title:
Engineering Plant Immunity: Using CRISPR/Cas9 to Generate Virus Resistance
Authors:
Zaidi, Syed Shan-e-Ali; Tashkandi, Manal; Mansoor, Shahid; Mahfouz, Magdy M. ( 0000-0002-0616-6365 )
Abstract:
Plant viruses infect many economically important crops, including wheat, cotton, maize, cassava, and other vegetables. These viruses pose a serious threat to agriculture worldwide, as decreases in cropland area per capita may cause production to fall short of that required to feed the increasing world population. Under these circumstances, conventional strategies can fail to control rapidly evolving and emerging plant viruses. Genome-engineering strategies have recently emerged as promising tools to introduce desirable traits in many eukaryotic species, including plants. Among these genome engineering technologies, the CRISPR (clustered regularly interspaced palindromic repeats)/CRISPR-associated 9 (CRISPR/Cas9) system has received special interest because of its simplicity, efficiency, and reproducibility. Recent studies have used CRISPR/Cas9 to engineer virus resistance in plants, either by directly targeting and cleaving the viral genome, or by modifying the host plant genome to introduce viral immunity. Here, we briefly describe the biology of the CRISPR/Cas9 system and plant viruses, and how different genome engineering technologies have been used to target these viruses. We further describe the main findings from recent studies of CRISPR/Cas9-mediated viral interference and discuss how these findings can be applied to improve global agriculture. We conclude by pinpointing the gaps in our knowledge and the outstanding questions regarding CRISPR/Cas9-mediated viral immunity.
KAUST Department:
Laboratory for Genome Engineering, Division of Biological Sciences, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia
Citation:
Zaidi SS-A, Tashkandi M, Mansoor S, Mahfouz MM (2016) Engineering Plant Immunity: Using CRISPR/Cas9 to Generate Virus Resistance. Frontiers in Plant Science 7. Available: http://dx.doi.org/10.3389/fpls.2016.01673.
Publisher:
Frontiers Media SA
Journal:
Frontiers in Plant Science
Issue Date:
8-Nov-2016
DOI:
10.3389/fpls.2016.01673
Type:
Article
ISSN:
1664-462X
Sponsors:
This work was funded by King Abdullah University of Science and Technology (KAUST).
Additional Links:
http://journal.frontiersin.org/article/10.3389/fpls.2016.01673/full
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorZaidi, Syed Shan-e-Alien
dc.contributor.authorTashkandi, Manalen
dc.contributor.authorMansoor, Shahiden
dc.contributor.authorMahfouz, Magdy M.en
dc.date.accessioned2017-01-30T13:02:47Z-
dc.date.available2017-01-30T13:02:47Z-
dc.date.issued2016-11-08en
dc.identifier.citationZaidi SS-A, Tashkandi M, Mansoor S, Mahfouz MM (2016) Engineering Plant Immunity: Using CRISPR/Cas9 to Generate Virus Resistance. Frontiers in Plant Science 7. Available: http://dx.doi.org/10.3389/fpls.2016.01673.en
dc.identifier.issn1664-462Xen
dc.identifier.doi10.3389/fpls.2016.01673en
dc.identifier.urihttp://hdl.handle.net/10754/622815-
dc.description.abstractPlant viruses infect many economically important crops, including wheat, cotton, maize, cassava, and other vegetables. These viruses pose a serious threat to agriculture worldwide, as decreases in cropland area per capita may cause production to fall short of that required to feed the increasing world population. Under these circumstances, conventional strategies can fail to control rapidly evolving and emerging plant viruses. Genome-engineering strategies have recently emerged as promising tools to introduce desirable traits in many eukaryotic species, including plants. Among these genome engineering technologies, the CRISPR (clustered regularly interspaced palindromic repeats)/CRISPR-associated 9 (CRISPR/Cas9) system has received special interest because of its simplicity, efficiency, and reproducibility. Recent studies have used CRISPR/Cas9 to engineer virus resistance in plants, either by directly targeting and cleaving the viral genome, or by modifying the host plant genome to introduce viral immunity. Here, we briefly describe the biology of the CRISPR/Cas9 system and plant viruses, and how different genome engineering technologies have been used to target these viruses. We further describe the main findings from recent studies of CRISPR/Cas9-mediated viral interference and discuss how these findings can be applied to improve global agriculture. We conclude by pinpointing the gaps in our knowledge and the outstanding questions regarding CRISPR/Cas9-mediated viral immunity.en
dc.description.sponsorshipThis work was funded by King Abdullah University of Science and Technology (KAUST).en
dc.publisherFrontiers Media SAen
dc.relation.urlhttp://journal.frontiersin.org/article/10.3389/fpls.2016.01673/fullen
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectplant virusen
dc.subjectCRISPR/Cas9en
dc.subjectgenome engineeringen
dc.subjectgeminivirusen
dc.subjectvirus resistanceen
dc.titleEngineering Plant Immunity: Using CRISPR/Cas9 to Generate Virus Resistanceen
dc.typeArticleen
dc.contributor.departmentLaboratory for Genome Engineering, Division of Biological Sciences, King Abdullah University of Science and Technology, Thuwal, Saudi Arabiaen
dc.identifier.journalFrontiers in Plant Scienceen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionNational Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistanen
kaust.authorZaidi, Syed Shan-e-Alien
kaust.authorTashkandi, Manalen
kaust.authorMahfouz, Magdy M.en
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