AtNPF2.5 Modulates Chloride (Cl−) Efflux from Roots of Arabidopsis thaliana

Handle URI:
http://hdl.handle.net/10754/622813
Title:
AtNPF2.5 Modulates Chloride (Cl−) Efflux from Roots of Arabidopsis thaliana
Authors:
Li, Bo; Qiu, Jiaen; Jayakannan, Maheswari; Xu, Bo; Li, Yuan; Mayo, Gwenda M.; Tester, Mark A. ( 0000-0002-5085-8801 ) ; Gilliham, Matthew; Roy, Stuart J.
Abstract:
The accumulation of high concentrations of chloride (Cl) in leaves can adversely affect plant growth. When comparing different varieties of the same Cl sensitive plant species those that exclude relatively more Cl from their shoots tend to perform better under saline conditions; however, the molecular mechanisms involved in maintaining low shoot Cl remain largely undefined. Recently, it was shown that the NRT1/PTR Family 2.4 protein (NPF2.4) loads Cl into the root xylem, which affects the accumulation of Cl in Arabidopsis shoots. Here we characterize NPF2.5, which is the closest homolog to NPF2.4 sharing 83.2% identity at the amino acid level. NPF2.5 is predominantly expressed in root cortical cells and its transcription is induced by salt. Functional characterisation of NPF2.5 via its heterologous expression in yeast (Saccharomyces cerevisiae) and Xenopus laevis oocytes indicated that NPF2.5 is likely to encode a Cl permeable transporter. Arabidopsis npf2.5 T-DNA knockout mutant plants exhibited a significantly lower Cl efflux from roots, and a greater Cl accumulation in shoots compared to salt-treated Col-0 wild-type plants. At the same time, NO- content in 3 the shoot remained unaffected. Accumulation of Cl in the shoot increased following (1) amiRNA-induced knockdown of NPF2.5 transcript abundance in the root, and (2) constitutive over-expression of NPF2.5. We suggest that both these findings are consistent with a role for NPF2.5 in modulating Cl transport. Based on these results, we propose that NPF2.5 functions as a pathway for Cl efflux from the root, contributing to exclusion of Cl from the shoot of Arabidopsis.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Li B, Qiu J, Jayakannan M, Xu B, Li Y, et al. (2017) AtNPF2.5 Modulates Chloride (Cl−) Efflux from Roots of Arabidopsis thaliana. Frontiers in Plant Science 7. Available: http://dx.doi.org/10.3389/fpls.2016.02013.
Publisher:
Frontiers Media SA
Journal:
Frontiers in Plant Science
Issue Date:
5-Jan-2017
DOI:
10.3389/fpls.2016.02013
Type:
Article
ISSN:
1664-462X
Sponsors:
This work was supported by: the Australian Research Council (ARC) and the Grains Research and Development Corporation (GRDC) to the Australian Centre for Plant Functional Genomics; ARC Centre of Excellence funding [grant number CE140100008] and ARC Future Fellowship [grant number FT130100709] to MG; ARC Discovery grant [grant number DP1095542], GRDC grant [grant number UA00118] and the King Abdullah University of Science and Technology grant to MT; GRDC grant to SR and MG [grant number UA00145]; and China Scholarship Council (CSC) Scholarship [grant number 2008618091] to BL.
Additional Links:
http://journal.frontiersin.org/article/10.3389/fpls.2016.02013/full
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLi, Boen
dc.contributor.authorQiu, Jiaenen
dc.contributor.authorJayakannan, Maheswarien
dc.contributor.authorXu, Boen
dc.contributor.authorLi, Yuanen
dc.contributor.authorMayo, Gwenda M.en
dc.contributor.authorTester, Mark A.en
dc.contributor.authorGilliham, Matthewen
dc.contributor.authorRoy, Stuart J.en
dc.date.accessioned2017-01-30T13:02:46Z-
dc.date.available2017-01-30T13:02:46Z-
dc.date.issued2017-01-05en
dc.identifier.citationLi B, Qiu J, Jayakannan M, Xu B, Li Y, et al. (2017) AtNPF2.5 Modulates Chloride (Cl−) Efflux from Roots of Arabidopsis thaliana. Frontiers in Plant Science 7. Available: http://dx.doi.org/10.3389/fpls.2016.02013.en
dc.identifier.issn1664-462Xen
dc.identifier.doi10.3389/fpls.2016.02013en
dc.identifier.urihttp://hdl.handle.net/10754/622813-
dc.description.abstractThe accumulation of high concentrations of chloride (Cl) in leaves can adversely affect plant growth. When comparing different varieties of the same Cl sensitive plant species those that exclude relatively more Cl from their shoots tend to perform better under saline conditions; however, the molecular mechanisms involved in maintaining low shoot Cl remain largely undefined. Recently, it was shown that the NRT1/PTR Family 2.4 protein (NPF2.4) loads Cl into the root xylem, which affects the accumulation of Cl in Arabidopsis shoots. Here we characterize NPF2.5, which is the closest homolog to NPF2.4 sharing 83.2% identity at the amino acid level. NPF2.5 is predominantly expressed in root cortical cells and its transcription is induced by salt. Functional characterisation of NPF2.5 via its heterologous expression in yeast (Saccharomyces cerevisiae) and Xenopus laevis oocytes indicated that NPF2.5 is likely to encode a Cl permeable transporter. Arabidopsis npf2.5 T-DNA knockout mutant plants exhibited a significantly lower Cl efflux from roots, and a greater Cl accumulation in shoots compared to salt-treated Col-0 wild-type plants. At the same time, NO- content in 3 the shoot remained unaffected. Accumulation of Cl in the shoot increased following (1) amiRNA-induced knockdown of NPF2.5 transcript abundance in the root, and (2) constitutive over-expression of NPF2.5. We suggest that both these findings are consistent with a role for NPF2.5 in modulating Cl transport. Based on these results, we propose that NPF2.5 functions as a pathway for Cl efflux from the root, contributing to exclusion of Cl from the shoot of Arabidopsis.en
dc.description.sponsorshipThis work was supported by: the Australian Research Council (ARC) and the Grains Research and Development Corporation (GRDC) to the Australian Centre for Plant Functional Genomics; ARC Centre of Excellence funding [grant number CE140100008] and ARC Future Fellowship [grant number FT130100709] to MG; ARC Discovery grant [grant number DP1095542], GRDC grant [grant number UA00118] and the King Abdullah University of Science and Technology grant to MT; GRDC grant to SR and MG [grant number UA00145]; and China Scholarship Council (CSC) Scholarship [grant number 2008618091] to BL.en
dc.publisherFrontiers Media SAen
dc.relation.urlhttp://journal.frontiersin.org/article/10.3389/fpls.2016.02013/fullen
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectArabidopsis thalianaen
dc.subjectChloride transporten
dc.subjectMIFEen
dc.subjectNPF2.5en
dc.subjectSalinity toleranceen
dc.subjectTEVCen
dc.titleAtNPF2.5 Modulates Chloride (Cl−) Efflux from Roots of Arabidopsis thalianaen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalFrontiers in Plant Scienceen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionAustralian Centre for Plant Functional Genomics, Adelaide, SA, Australiaen
dc.contributor.institutionSchool of Agriculture, Food and Wine, Waite Research Institute, University of Adelaide, Adelaide, SA, Australiaen
dc.contributor.institutionARC Centre of Excellence in Plant Energy Biology, Adelaide, SA, Australiaen
kaust.authorLi, Boen
kaust.authorTester, Mark A.en
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.