Mesenchymal stem cells cultured on magnetic nanowire substrates

Handle URI:
http://hdl.handle.net/10754/622787
Title:
Mesenchymal stem cells cultured on magnetic nanowire substrates
Authors:
Perez, Jose E. ( 0000-0002-2206-0034 ) ; Ravasi, Timothy ( 0000-0002-9950-465X ) ; Kosel, Jürgen ( 0000-0002-8998-8275 )
Abstract:
Stem cells have been shown to respond to extracellular mechanical stimuli by regulating their fate through the activation of specific signaling pathways. In this work, an array of iron nanowires (NWs) aligned perpendicularly to the surface was fabricated by pulsed electrodepositon in porous alumina templates followed by a partial removal of the alumina to reveal 2-3 μm of the NWs. This resulted in alumina substrates with densely arranged NWs of 33 nm in diameter separated by 100 nm. The substrates were characterized by scanning electron microscopy (SEM) energy dispersive x-ray analysis and vibrating sample magnetometer. The NW array was then used as a platform for the culture of human mesenchymal stem cells (hMSCs). The cells were stained for the cell nucleus and actin filaments, as well as immuno-stained for the focal adhesion protein vinculin, and then observed by fluorescence microscopy in order to characterize their spreading behavior. Calcein AM/ethidium homodimer-1 staining allowed the determination of cell viability. The interface between the cells and the NWs was studied using SEM. Results showed that hMSCs underwent a re-organization of actin filaments that translated into a change from an elongated to a spherical cell shape. Actin filaments and vinculin accumulated in bundles, suggesting the attachment and formation of focal adhesion points of the cells on the NWs. Though the overall number of cells attached on the NWs was lower compared to the control, the attached cells maintained a high viability (>90%) for up to 6 d. Analysis of the interface between the NWs and the cells confirmed the re-organization of F-actin and revealed the adhesion points of the cells on the NWs. Additionally, a net of filopodia surrounded each cell, suggesting the probing of the array to find additional adhesion points. The cells maintained their round shape for up to 6 d of culture. Overall, the NW array is a promising nanostructured platform for studying and influencing hMSCs differentiation.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Citation:
Perez JE, Ravasi T, Kosel J (2016) Mesenchymal stem cells cultured on magnetic nanowire substrates. Nanotechnology 28: 055703. Available: http://dx.doi.org/10.1088/1361-6528/aa52a3.
Publisher:
IOP Publishing
Journal:
Nanotechnology
Issue Date:
28-Dec-2016
DOI:
10.1088/1361-6528/aa52a3
Type:
Article
ISSN:
0957-4484; 1361-6528
Sponsors:
Research reported in this publication was supported by the King Abdullah University of Science and Technology (KAUST).
Additional Links:
http://iopscience.iop.org/article/10.1088/1361-6528/aa52a3/meta;jsessionid=227AD3FA331E525E13535E508C947DF4.c2.iopscience.cld.iop.org
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorPerez, Jose E.en
dc.contributor.authorRavasi, Timothyen
dc.contributor.authorKosel, Jürgenen
dc.date.accessioned2017-01-29T13:51:39Z-
dc.date.available2017-01-29T13:51:39Z-
dc.date.issued2016-12-28en
dc.identifier.citationPerez JE, Ravasi T, Kosel J (2016) Mesenchymal stem cells cultured on magnetic nanowire substrates. Nanotechnology 28: 055703. Available: http://dx.doi.org/10.1088/1361-6528/aa52a3.en
dc.identifier.issn0957-4484en
dc.identifier.issn1361-6528en
dc.identifier.doi10.1088/1361-6528/aa52a3en
dc.identifier.urihttp://hdl.handle.net/10754/622787-
dc.description.abstractStem cells have been shown to respond to extracellular mechanical stimuli by regulating their fate through the activation of specific signaling pathways. In this work, an array of iron nanowires (NWs) aligned perpendicularly to the surface was fabricated by pulsed electrodepositon in porous alumina templates followed by a partial removal of the alumina to reveal 2-3 μm of the NWs. This resulted in alumina substrates with densely arranged NWs of 33 nm in diameter separated by 100 nm. The substrates were characterized by scanning electron microscopy (SEM) energy dispersive x-ray analysis and vibrating sample magnetometer. The NW array was then used as a platform for the culture of human mesenchymal stem cells (hMSCs). The cells were stained for the cell nucleus and actin filaments, as well as immuno-stained for the focal adhesion protein vinculin, and then observed by fluorescence microscopy in order to characterize their spreading behavior. Calcein AM/ethidium homodimer-1 staining allowed the determination of cell viability. The interface between the cells and the NWs was studied using SEM. Results showed that hMSCs underwent a re-organization of actin filaments that translated into a change from an elongated to a spherical cell shape. Actin filaments and vinculin accumulated in bundles, suggesting the attachment and formation of focal adhesion points of the cells on the NWs. Though the overall number of cells attached on the NWs was lower compared to the control, the attached cells maintained a high viability (>90%) for up to 6 d. Analysis of the interface between the NWs and the cells confirmed the re-organization of F-actin and revealed the adhesion points of the cells on the NWs. Additionally, a net of filopodia surrounded each cell, suggesting the probing of the array to find additional adhesion points. The cells maintained their round shape for up to 6 d of culture. Overall, the NW array is a promising nanostructured platform for studying and influencing hMSCs differentiation.en
dc.description.sponsorshipResearch reported in this publication was supported by the King Abdullah University of Science and Technology (KAUST).en
dc.publisherIOP Publishingen
dc.relation.urlhttp://iopscience.iop.org/article/10.1088/1361-6528/aa52a3/meta;jsessionid=227AD3FA331E525E13535E508C947DF4.c2.iopscience.cld.iop.orgen
dc.subjectbiocompatibilityen
dc.subjectcell cultureen
dc.subjectmagnetic nanowiresen
dc.subjectmesenchymal stem cellsen
dc.titleMesenchymal stem cells cultured on magnetic nanowire substratesen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.identifier.journalNanotechnologyen
kaust.authorPerez, Jose E.en
kaust.authorRavasi, Timothyen
kaust.authorKosel, Jürgenen
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