Pre-mRNA splicing repression triggers abiotic stress signaling in plants

Handle URI:
http://hdl.handle.net/10754/622750
Title:
Pre-mRNA splicing repression triggers abiotic stress signaling in plants
Authors:
Ling, Yu ( 0000-0002-8067-523X ) ; Alshareef, Sahar ( 0000-0002-0377-1113 ) ; Butt, Haroon; Lozano-Juste, Jorge; Li, Lixin; Galal, Aya A.; Moustafa, Ahmed; Momin, Afaque Ahmad Imtiyaz; Tashkandi, Manal; Richardson, Dale N.; Fujii, Hiroaki; Arold, Stefan T. ( 0000-0001-5278-0668 ) ; Rodriguez, Pedro L.; Duque, Paula; Mahfouz, Magdy M. ( 0000-0002-0616-6365 )
Abstract:
Alternative splicing (AS) of precursor RNAs enhances transcriptome plasticity and proteome diversity in response to diverse growth and stress cues. Recent work has shown that AS is pervasive across plant species, with more than 60% of intron-containing genes producing different isoforms. Mammalian cell-based assays have discovered various inhibitors of AS. Here, we show that the macrolide pladienolide B (PB) inhibits constitutive splicing and AS in plants. Also, our RNA sequencing (RNA-seq) data revealed that PB mimics abiotic stress signals including salt, drought and abscisic acid (ABA). PB activates the abiotic stress- and ABA-responsive reporters RD29A
KAUST Department:
Laboratory for Genome Engineering; Division of Biological Sciences; 4700 King Abdullah University of Science and Technology; Thuwal 23955-6900 Saudi Arabia; Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Ling Y, Alshareef S, Butt H, Lozano-Juste J, Li L, et al. (2017) Pre-mRNA splicing repression triggers abiotic stress signaling in plants. The Plant Journal. Available: http://dx.doi.org/10.1111/tpj.13383.
Publisher:
Wiley-Blackwell
Journal:
The Plant Journal
Issue Date:
24-Sep-2016
DOI:
10.1111/tpj.13383
Type:
Article
ISSN:
0960-7412
Sponsors:
We wish to thank members of the Laboratory for Genome Engineering at King Abdullah University of Science and Technology for helpful discussions and comments on the manuscript. We wish to thank Moussa Benhamed for helpful discussions and suggestions and for providing key materials. We wish to thank Sean Cutler for providing Arabidopsis seeds of MAKPKKK18-uidA. This study was supported by King Abdullah University of Science and Technology. Work in PR's laboratory was funded by grant BIO2014-52537-R from MINECO. Work in PD's laboratory is funded by grant PTDC/BIA-PLA/1084/2014 from FCT. The authors declare no conflicts of interest.
Additional Links:
http://onlinelibrary.wiley.com/doi/10.1111/tpj.13383/full
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLing, Yuen
dc.contributor.authorAlshareef, Saharen
dc.contributor.authorButt, Haroonen
dc.contributor.authorLozano-Juste, Jorgeen
dc.contributor.authorLi, Lixinen
dc.contributor.authorGalal, Aya A.en
dc.contributor.authorMoustafa, Ahmeden
dc.contributor.authorMomin, Afaque Ahmad Imtiyazen
dc.contributor.authorTashkandi, Manalen
dc.contributor.authorRichardson, Dale N.en
dc.contributor.authorFujii, Hiroakien
dc.contributor.authorArold, Stefan T.en
dc.contributor.authorRodriguez, Pedro L.en
dc.contributor.authorDuque, Paulaen
dc.contributor.authorMahfouz, Magdy M.en
dc.date.accessioned2017-01-29T13:51:36Z-
dc.date.available2017-01-29T13:51:36Z-
dc.date.issued2016-09-24en
dc.identifier.citationLing Y, Alshareef S, Butt H, Lozano-Juste J, Li L, et al. (2017) Pre-mRNA splicing repression triggers abiotic stress signaling in plants. The Plant Journal. Available: http://dx.doi.org/10.1111/tpj.13383.en
dc.identifier.issn0960-7412en
dc.identifier.doi10.1111/tpj.13383en
dc.identifier.urihttp://hdl.handle.net/10754/622750-
dc.description.abstractAlternative splicing (AS) of precursor RNAs enhances transcriptome plasticity and proteome diversity in response to diverse growth and stress cues. Recent work has shown that AS is pervasive across plant species, with more than 60% of intron-containing genes producing different isoforms. Mammalian cell-based assays have discovered various inhibitors of AS. Here, we show that the macrolide pladienolide B (PB) inhibits constitutive splicing and AS in plants. Also, our RNA sequencing (RNA-seq) data revealed that PB mimics abiotic stress signals including salt, drought and abscisic acid (ABA). PB activates the abiotic stress- and ABA-responsive reporters RD29Aen
dc.description.sponsorshipWe wish to thank members of the Laboratory for Genome Engineering at King Abdullah University of Science and Technology for helpful discussions and comments on the manuscript. We wish to thank Moussa Benhamed for helpful discussions and suggestions and for providing key materials. We wish to thank Sean Cutler for providing Arabidopsis seeds of MAKPKKK18-uidA. This study was supported by King Abdullah University of Science and Technology. Work in PR's laboratory was funded by grant BIO2014-52537-R from MINECO. Work in PD's laboratory is funded by grant PTDC/BIA-PLA/1084/2014 from FCT. The authors declare no conflicts of interest.en
dc.publisherWiley-Blackwellen
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1111/tpj.13383/fullen
dc.subjectAbscisic aciden
dc.subjectAlternative splicingen
dc.subjectAbiotic Stress Responsesen
dc.subjectSerine/arginine-rich Proteinsen
dc.subjectPladienolide Ben
dc.subjectSplicing Inhibitorsen
dc.titlePre-mRNA splicing repression triggers abiotic stress signaling in plantsen
dc.typeArticleen
dc.contributor.departmentLaboratory for Genome Engineering; Division of Biological Sciences; 4700 King Abdullah University of Science and Technology; Thuwal 23955-6900 Saudi Arabiaen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalThe Plant Journalen
dc.contributor.institutionInstituto de Biologia Molecular y Celular de Plantas; Consejo Superior de Investigaciones Cientificas-Universidad Politecnica de Valencia; ES-46022 Valencia Spainen
dc.contributor.institutionDepartment of Biology and Biotechnology Graduate Program; American University in Cairo; New Cairo 11835 Egypten
dc.contributor.institutionInstituto Gulbenkian de Ciência; Rua da Quinta Grande 6 2780-156 Oeiras Portugalen
dc.contributor.institutionMolecular Plant Biology; Department of Biochemistry; University of Turku; FI-20014 Turku Finlanden
kaust.authorLing, Yuen
kaust.authorAlshareef, Saharen
kaust.authorButt, Haroonen
kaust.authorLi, Lixinen
kaust.authorMomin, Afaque Ahmad Imtiyazen
kaust.authorTashkandi, Manalen
kaust.authorArold, Stefan T.en
kaust.authorMahfouz, Magdy M.en
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