Bacterial diversity shift determined by different diets in the gut of the spotted wing fly Drosophila suzukii is primarily reflected on acetic acid bacteria

Handle URI:
http://hdl.handle.net/10754/622736
Title:
Bacterial diversity shift determined by different diets in the gut of the spotted wing fly Drosophila suzukii is primarily reflected on acetic acid bacteria
Authors:
Vacchini, Violetta; Gonella, Elena; Crotti, Elena; Prosdocimi, Erica M.; Mazzetto, Fabio; Chouaia, Bessem; Callegari, Matteo; Mapelli, Francesca; Mandrioli, Mauro; Alma, Alberto; Daffonchio, Daniele ( 0000-0003-0947-925X )
Abstract:
The pivotal role of diet in shaping gut microbiota has been evaluated in different animal models, including insects. Drosophila flies harbour an inconstant microbiota among which acetic acid bacteria (AAB) are important components. Here, we investigated the bacterial and AAB components of the invasive pest Drosophila suzukii microbiota, by studying the same insect population separately grown on fruit-based or non-fruit artificial diet. AAB were highly prevalent in the gut under both diets (90 and 92% infection rates with fruits and artificial diet, respectively). Fluorescent in situ hybridization and recolonization experiments with green fluorescent protein (Gfp)-labelled strains showed AAB capability to massively colonize insect gut. High-throughput sequencing on 16S rRNA gene indicated that the bacterial microbiota of guts fed with the two diets clustered separately. By excluding AAB-related OTUs from the analysis, insect bacterial communities did not cluster separately according to the diet, suggesting that diet-based diversification of the community is primarily reflected on the AAB component of the community. Diet influenced also AAB alpha-diversity, with separate OTU distributions based on diets. High prevalence, localization and massive recolonization, together with AAB clustering behaviour in relation to diet, suggest an AAB role in the D. suzukii gut response to diet modification. This article is protected by copyright. All rights reserved.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Vacchini V, Gonella E, Crotti E, Prosdocimi EM, Mazzetto F, et al. (2016) Bacterial diversity shift determined by different diets in the gut of the spotted wing fly Drosophila suzukii is primarily reflected on acetic acid bacteria. Environmental Microbiology Reports. Available: http://dx.doi.org/10.1111/1758-2229.12505.
Publisher:
Wiley-Blackwell
Journal:
Environmental Microbiology Reports
Issue Date:
25-Nov-2016
DOI:
10.1111/1758-2229.12505
Type:
Article
ISSN:
1758-2229
Sponsors:
King Abdullah University of Science and Technology supported the study through the baseline research funds to D.D. This work was partially funded by Consorzio di Ricerca Sperimentazione e Divulgazione per l’Ortofrutticoltura Piemontese, within the project “Programma di ricerca, sperimentazione e dimostrazione agricola in frutticoltura e orticoltura – 2014 – Indagini sul nuovo dittero esotico Drosophila suzukii responsabile di gravi danni alle drupacee”. E.C. acknowledges personal support from “Piano Sviluppo di Ateneo: Linea B-Dotazione annuale per attività istituzionale” in the project “Acetic acid bacteria cell factories”.
Additional Links:
http://onlinelibrary.wiley.com/doi/10.1111/1758-2229.12505/abstract
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorVacchini, Violettaen
dc.contributor.authorGonella, Elenaen
dc.contributor.authorCrotti, Elenaen
dc.contributor.authorProsdocimi, Erica M.en
dc.contributor.authorMazzetto, Fabioen
dc.contributor.authorChouaia, Bessemen
dc.contributor.authorCallegari, Matteoen
dc.contributor.authorMapelli, Francescaen
dc.contributor.authorMandrioli, Mauroen
dc.contributor.authorAlma, Albertoen
dc.contributor.authorDaffonchio, Danieleen
dc.date.accessioned2017-01-26T13:29:24Z-
dc.date.available2017-01-26T13:29:24Z-
dc.date.issued2016-11-25en
dc.identifier.citationVacchini V, Gonella E, Crotti E, Prosdocimi EM, Mazzetto F, et al. (2016) Bacterial diversity shift determined by different diets in the gut of the spotted wing fly Drosophila suzukii is primarily reflected on acetic acid bacteria. Environmental Microbiology Reports. Available: http://dx.doi.org/10.1111/1758-2229.12505.en
dc.identifier.issn1758-2229en
dc.identifier.doi10.1111/1758-2229.12505en
dc.identifier.urihttp://hdl.handle.net/10754/622736-
dc.description.abstractThe pivotal role of diet in shaping gut microbiota has been evaluated in different animal models, including insects. Drosophila flies harbour an inconstant microbiota among which acetic acid bacteria (AAB) are important components. Here, we investigated the bacterial and AAB components of the invasive pest Drosophila suzukii microbiota, by studying the same insect population separately grown on fruit-based or non-fruit artificial diet. AAB were highly prevalent in the gut under both diets (90 and 92% infection rates with fruits and artificial diet, respectively). Fluorescent in situ hybridization and recolonization experiments with green fluorescent protein (Gfp)-labelled strains showed AAB capability to massively colonize insect gut. High-throughput sequencing on 16S rRNA gene indicated that the bacterial microbiota of guts fed with the two diets clustered separately. By excluding AAB-related OTUs from the analysis, insect bacterial communities did not cluster separately according to the diet, suggesting that diet-based diversification of the community is primarily reflected on the AAB component of the community. Diet influenced also AAB alpha-diversity, with separate OTU distributions based on diets. High prevalence, localization and massive recolonization, together with AAB clustering behaviour in relation to diet, suggest an AAB role in the D. suzukii gut response to diet modification. This article is protected by copyright. All rights reserved.en
dc.description.sponsorshipKing Abdullah University of Science and Technology supported the study through the baseline research funds to D.D. This work was partially funded by Consorzio di Ricerca Sperimentazione e Divulgazione per l’Ortofrutticoltura Piemontese, within the project “Programma di ricerca, sperimentazione e dimostrazione agricola in frutticoltura e orticoltura – 2014 – Indagini sul nuovo dittero esotico Drosophila suzukii responsabile di gravi danni alle drupacee”. E.C. acknowledges personal support from “Piano Sviluppo di Ateneo: Linea B-Dotazione annuale per attività istituzionale” in the project “Acetic acid bacteria cell factories”.en
dc.publisherWiley-Blackwellen
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1111/1758-2229.12505/abstracten
dc.rightsThis is the peer reviewed version of the following article: Vacchini, V., Gonella, E., Crotti, E., Prosdocimi, E. M., Mazzetto, F., Chouaia, B., Callegari, M., Mapelli, F., Mandrioli, M., Alma, A. and Daffonchio, D. (2016), Bacterial diversity shift determined by different diets in the gut of the spotted wing fly Drosophila suzukii is primarily reflected on acetic acid bacteria. Environmental Microbiology Reports. Accepted Author Manuscript. doi:10.1111/1758-2229.12505, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/1758-2229.12505/abstract. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.en
dc.subjectGreen fluorescent proteinen
dc.subjectSymbiontsen
dc.subject16S Rrna Gene Pyrosequencingen
dc.subjectCultivation-dependent Approachen
dc.subjectFluorescent In Situ Hybridization (Fish)en
dc.titleBacterial diversity shift determined by different diets in the gut of the spotted wing fly Drosophila suzukii is primarily reflected on acetic acid bacteriaen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalEnvironmental Microbiology Reportsen
dc.eprint.versionPost-printen
dc.contributor.institutionDipartimento di Scienze per gli Alimenti, la Nutrizione e l'Ambiente (DeFENS); Università degli Studi di Milano; Milano Italyen
dc.contributor.institutionDipartimento di Scienze Agrarie, Forestali e Alimentari (DISAFA); Università degli Studi di Torino; Grugliasco Italyen
dc.contributor.institutionDipartimento di Scienze della Vita (DSV); Università degli Studi di Modena e Reggio Emilia; Modena Italyen
kaust.authorDaffonchio, Danieleen
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