Distinct Bacterial Communities Associated with the Coral Model Aiptasia in Aposymbiotic and Symbiotic States with Symbiodinium

Handle URI:
http://hdl.handle.net/10754/622707
Title:
Distinct Bacterial Communities Associated with the Coral Model Aiptasia in Aposymbiotic and Symbiotic States with Symbiodinium
Authors:
Röthig, Till ( 0000-0001-6359-8589 ) ; Costa, Rúben M.; Simona, Fabia; Baumgarten, Sebastian ( 0000-0003-2646-7699 ) ; Torres, Ana F.; Radhakrishnan, Anand; Aranda, Manuel ( 0000-0001-6673-016X ) ; Voolstra, Christian R. ( 0000-0003-4555-3795 )
Abstract:
Coral reefs are in decline. The basic functional unit of coral reefs is the coral metaorganism or holobiont consisting of the cnidarian host animal, symbiotic algae of the genus Symbiodinium, and a specific consortium of bacteria (among others), but research is slow due to the difficulty of working with corals. Aiptasia has proven to be a tractable model system to elucidate the intricacies of cnidarian-dinoflagellate symbioses, but characterization of the associated bacterial microbiome is required to provide a complete and integrated understanding of holobiont function. In this work, we characterize and analyze the microbiome of aposymbiotic and symbiotic Aiptasia and show that bacterial associates are distinct in both conditions. We further show that key microbial associates can be cultured without their cnidarian host. Our results suggest that bacteria play an important role in the symbiosis of Aiptasia with Symbiodinium, a finding that underlines the power of the Aiptasia model system where cnidarian hosts can be analyzed in aposymbiotic and symbiotic states. The characterization of the native microbiome and the ability to retrieve culturable isolates contributes to the resources available for the Aiptasia model system. This provides an opportunity to comparatively analyze cnidarian metaorganisms as collective functional holobionts and as separated member species. We hope that this will accelerate research into understanding the intricacies of coral biology, which is urgently needed to develop strategies to mitigate the effects of environmental change.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Red Sea Research Center (RSRC)
Citation:
Röthig T, Costa RM, Simona F, Baumgarten S, Torres AF, et al. (2016) Distinct Bacterial Communities Associated with the Coral Model Aiptasia in Aposymbiotic and Symbiotic States with Symbiodinium. Frontiers in Marine Science 3. Available: http://dx.doi.org/10.3389/fmars.2016.00234.
Publisher:
Frontiers Media SA
Journal:
Frontiers in Marine Science
Issue Date:
18-Nov-2016
DOI:
10.3389/fmars.2016.00234
Type:
Article
ISSN:
2296-7745
Sponsors:
We would like to thank Mohammad Bin Sarhan for preliminary work on cultural isolates and Craig T. Michell for MiSeq library generation.
Additional Links:
journal.frontiersin.org/article/10.3389/fmars.2016.00234/full
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorRöthig, Tillen
dc.contributor.authorCosta, Rúben M.en
dc.contributor.authorSimona, Fabiaen
dc.contributor.authorBaumgarten, Sebastianen
dc.contributor.authorTorres, Ana F.en
dc.contributor.authorRadhakrishnan, Ananden
dc.contributor.authorAranda, Manuelen
dc.contributor.authorVoolstra, Christian R.en
dc.date.accessioned2017-01-22T10:45:41Z-
dc.date.available2017-01-22T10:45:41Z-
dc.date.issued2016-11-18en
dc.identifier.citationRöthig T, Costa RM, Simona F, Baumgarten S, Torres AF, et al. (2016) Distinct Bacterial Communities Associated with the Coral Model Aiptasia in Aposymbiotic and Symbiotic States with Symbiodinium. Frontiers in Marine Science 3. Available: http://dx.doi.org/10.3389/fmars.2016.00234.en
dc.identifier.issn2296-7745en
dc.identifier.doi10.3389/fmars.2016.00234en
dc.identifier.urihttp://hdl.handle.net/10754/622707-
dc.description.abstractCoral reefs are in decline. The basic functional unit of coral reefs is the coral metaorganism or holobiont consisting of the cnidarian host animal, symbiotic algae of the genus Symbiodinium, and a specific consortium of bacteria (among others), but research is slow due to the difficulty of working with corals. Aiptasia has proven to be a tractable model system to elucidate the intricacies of cnidarian-dinoflagellate symbioses, but characterization of the associated bacterial microbiome is required to provide a complete and integrated understanding of holobiont function. In this work, we characterize and analyze the microbiome of aposymbiotic and symbiotic Aiptasia and show that bacterial associates are distinct in both conditions. We further show that key microbial associates can be cultured without their cnidarian host. Our results suggest that bacteria play an important role in the symbiosis of Aiptasia with Symbiodinium, a finding that underlines the power of the Aiptasia model system where cnidarian hosts can be analyzed in aposymbiotic and symbiotic states. The characterization of the native microbiome and the ability to retrieve culturable isolates contributes to the resources available for the Aiptasia model system. This provides an opportunity to comparatively analyze cnidarian metaorganisms as collective functional holobionts and as separated member species. We hope that this will accelerate research into understanding the intricacies of coral biology, which is urgently needed to develop strategies to mitigate the effects of environmental change.en
dc.description.sponsorshipWe would like to thank Mohammad Bin Sarhan for preliminary work on cultural isolates and Craig T. Michell for MiSeq library generation.en
dc.publisherFrontiers Media SAen
dc.relation.urljournal.frontiersin.org/article/10.3389/fmars.2016.00234/fullen
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subject16S rRNA geneen
dc.subjectCnidarian-dinoflagellate symbiosisen
dc.subjectCoral reefen
dc.subjectFunctional profilingen
dc.subjectMicrobial community profilingen
dc.titleDistinct Bacterial Communities Associated with the Coral Model Aiptasia in Aposymbiotic and Symbiotic States with Symbiodiniumen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalFrontiers in Marine Scienceen
dc.eprint.versionPublisher's Version/PDFen
kaust.authorRöthig, Tillen
kaust.authorCosta, Rúben M.en
kaust.authorSimona, Fabiaen
kaust.authorBaumgarten, Sebastianen
kaust.authorTorres, Ana F.en
kaust.authorRadhakrishnan, Ananden
kaust.authorAranda, Manuelen
kaust.authorVoolstra, Christian R.en
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