Arabidopsis SUMO protease ASP1 positively regulates flowering time partially through regulating FLC stability 

Handle URI:
http://hdl.handle.net/10754/622703
Title:
Arabidopsis SUMO protease ASP1 positively regulates flowering time partially through regulating FLC stability 
Authors:
Kong, Xiangxiong; Luo, Xi; Qu, Gao Ping; Liu, Peng; Jin, Jing Bo
Abstract:
The initiation of flowering is tightly regulated by the endogenous and environment signals, which is crucial for the reproductive success of flowering plants. It is well known that autonomous and vernalization pathways repress transcription of FLOWERING LOCUS C (FLC), a focal floral repressor, but how its protein stability is regulated remains largely unknown. Here, we found that mutations in a novel Arabidopsis SUMO protease 1 (ASP1) resulted in a strong late-flowering phenotype under long-days, but to a lesser extent under short-days. ASP1 localizes in the nucleus and exhibited a SUMO protease activity in vitro and in vivo. The conserved Cys-577 in ASP1 is critical for its enzymatic activity, as well as its physiological function in the regulation of flowering time. Genetic and gene expression analyses demonstrated that ASP1 promotes transcription of positive regulators of flowering, such as FT, SOC1 and FD, and may function in both CO-dependent photoperiod pathway and FLC-dependent pathways. Although the transcription level of FLC was not affected in the loss-of-function asp1 mutant, the protein stability of FLC was increased in the asp1 mutant. Taken together, this study identified a novel bona fide SUMO protease, ASP1, which positively regulates transition to flowering at least partly by repressing FLC protein stability.
KAUST Department:
Division of Biological Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia
Citation:
Kong X, Luo X, Qu G-P, Liu P, Jin JB (2017) Arabidopsis SUMO protease ASP1 positively regulates flowering time partially through regulating FLC stability . Journal of Integrative Plant Biology 59: 15–29. Available: http://dx.doi.org/10.1111/jipb.12509.
Publisher:
Wiley-Blackwell
Journal:
Journal of Integrative Plant Biology
Issue Date:
7-Dec-2016
DOI:
10.1111/jipb.12509
Type:
Article
ISSN:
1672-9072
Sponsors:
This work was supported by grants from the National Natural Science Foundation of China (31301166 for P.L. and 31471363 for J.B.J), the Ministry of Science and Technology of the People's Republic of China (2012CB114302 for J.B.J) and the Chinese Academy of Sciences (XDA08010105 for J.B.J).
Additional Links:
http://onlinelibrary.wiley.com/doi/10.1111/jipb.12509/abstract
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorKong, Xiangxiongen
dc.contributor.authorLuo, Xien
dc.contributor.authorQu, Gao Pingen
dc.contributor.authorLiu, Pengen
dc.contributor.authorJin, Jing Boen
dc.date.accessioned2017-01-22T10:45:41Z-
dc.date.available2017-01-22T10:45:41Z-
dc.date.issued2016-12-07en
dc.identifier.citationKong X, Luo X, Qu G-P, Liu P, Jin JB (2017) Arabidopsis SUMO protease ASP1 positively regulates flowering time partially through regulating FLC stability . Journal of Integrative Plant Biology 59: 15–29. Available: http://dx.doi.org/10.1111/jipb.12509.en
dc.identifier.issn1672-9072en
dc.identifier.doi10.1111/jipb.12509en
dc.identifier.urihttp://hdl.handle.net/10754/622703-
dc.description.abstractThe initiation of flowering is tightly regulated by the endogenous and environment signals, which is crucial for the reproductive success of flowering plants. It is well known that autonomous and vernalization pathways repress transcription of FLOWERING LOCUS C (FLC), a focal floral repressor, but how its protein stability is regulated remains largely unknown. Here, we found that mutations in a novel Arabidopsis SUMO protease 1 (ASP1) resulted in a strong late-flowering phenotype under long-days, but to a lesser extent under short-days. ASP1 localizes in the nucleus and exhibited a SUMO protease activity in vitro and in vivo. The conserved Cys-577 in ASP1 is critical for its enzymatic activity, as well as its physiological function in the regulation of flowering time. Genetic and gene expression analyses demonstrated that ASP1 promotes transcription of positive regulators of flowering, such as FT, SOC1 and FD, and may function in both CO-dependent photoperiod pathway and FLC-dependent pathways. Although the transcription level of FLC was not affected in the loss-of-function asp1 mutant, the protein stability of FLC was increased in the asp1 mutant. Taken together, this study identified a novel bona fide SUMO protease, ASP1, which positively regulates transition to flowering at least partly by repressing FLC protein stability.en
dc.description.sponsorshipThis work was supported by grants from the National Natural Science Foundation of China (31301166 for P.L. and 31471363 for J.B.J), the Ministry of Science and Technology of the People's Republic of China (2012CB114302 for J.B.J) and the Chinese Academy of Sciences (XDA08010105 for J.B.J).en
dc.publisherWiley-Blackwellen
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1111/jipb.12509/abstracten
dc.rightsThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.en
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en
dc.subjectCONSTANSen
dc.subjectFLOWERING LOCUS Cen
dc.subjectflowering timeen
dc.subjectsmall ubiquitin-related modifier (SUMO)en
dc.subjectSUMO proteaseen
dc.titleArabidopsis SUMO protease ASP1 positively regulates flowering time partially through regulating FLC stability en
dc.typeArticleen
dc.contributor.departmentDivision of Biological Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabiaen
dc.identifier.journalJournal of Integrative Plant Biologyen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionKey Laboratory of Plant Molecular Physiology; Institute of Botany; the Chinese Academy of Sciences; Beijing 100093 Chinaen
dc.contributor.institutionGraduate School of the Chinese Academy of Sciences; Beijing 100049 Chinaen
kaust.authorLiu, Pengen
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