Hydrocarbonoclastic Alcanivorax Isolates Exhibit Different Physiological and Expression Responses to n-dodecane

Handle URI:
http://hdl.handle.net/10754/622690
Title:
Hydrocarbonoclastic Alcanivorax Isolates Exhibit Different Physiological and Expression Responses to n-dodecane
Authors:
Barbato, Marta; Scoma, Alberto; Mapelli, Francesca; De Smet, Rebecca; Banat, Ibrahim M.; Daffonchio, Daniele ( 0000-0003-0947-925X ) ; Boon, Nico; Borin, Sara
Abstract:
Autochthonous microorganisms inhabiting hydrocarbon polluted marine environments play a fundamental role in natural attenuation and constitute promising resources for bioremediation approaches. Alcanivorax spp. members are ubiquitous in contaminated surface waters and are the first to flourish on a wide range of alkanes after an oil-spill. Following oil contamination, a transient community of different Alcanivorax spp. develop, but whether they use a similar physiological, cellular and transcriptomic response to hydrocarbon substrates is unknown. In order to identify which cellular mechanisms are implicated in alkane degradation, we investigated the response of two isolates belonging to different Alcanivorax species, A. dieselolei KS 293 and A. borkumensis SK2 growing on n-dodecane (C12) or on pyruvate. Both strains were equally able to grow on C12 but they activated different strategies to exploit it as carbon and energy source. The membrane morphology and hydrophobicity of SK2 changed remarkably, from neat and hydrophilic on pyruvate to indented and hydrophobic on C12, while no changes were observed in KS 293. In addition, SK2 accumulated a massive amount of intracellular grains when growing on pyruvate, which might constitute a carbon reservoir. Furthermore, SK2 significantly decreased medium surface tension with respect to KS 293 when growing on C12, as a putative result of higher production of biosurfactants. The transcriptomic responses of the two isolates were also highly different. KS 293 changes were relatively balanced when growing on C12 with respect to pyruvate, giving almost the same amount of upregulated (28%), downregulated (37%) and equally regulated (36%) genes, while SK2 transcription was upregulated for most of the genes (81%) when growing on pyruvate when compared to C12. While both strains, having similar genomic background in genes related to hydrocarbon metabolism, retained the same capability to grow on C12, they nevertheless presented very different physiological, cellular and transcriptomic landscapes.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Barbato M, Scoma A, Mapelli F, De Smet R, Banat IM, et al. (2016) Hydrocarbonoclastic Alcanivorax Isolates Exhibit Different Physiological and Expression Responses to n-dodecane. Frontiers in Microbiology 7. Available: http://dx.doi.org/10.3389/fmicb.2016.02056.
Publisher:
Frontiers Media SA
Journal:
Frontiers in Microbiology
Issue Date:
21-Dec-2016
DOI:
10.3389/fmicb.2016.02056
Type:
Article
ISSN:
1664-302X
Sponsors:
This work was funded by FP-7 project Kill Spill (No. 312139,
Additional Links:
http://journal.frontiersin.org/article/10.3389/fmicb.2016.02056/full
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorBarbato, Martaen
dc.contributor.authorScoma, Albertoen
dc.contributor.authorMapelli, Francescaen
dc.contributor.authorDe Smet, Rebeccaen
dc.contributor.authorBanat, Ibrahim M.en
dc.contributor.authorDaffonchio, Danieleen
dc.contributor.authorBoon, Nicoen
dc.contributor.authorBorin, Saraen
dc.date.accessioned2017-01-15T13:40:35Z-
dc.date.available2017-01-15T13:40:35Z-
dc.date.issued2016-12-21en
dc.identifier.citationBarbato M, Scoma A, Mapelli F, De Smet R, Banat IM, et al. (2016) Hydrocarbonoclastic Alcanivorax Isolates Exhibit Different Physiological and Expression Responses to n-dodecane. Frontiers in Microbiology 7. Available: http://dx.doi.org/10.3389/fmicb.2016.02056.en
dc.identifier.issn1664-302Xen
dc.identifier.doi10.3389/fmicb.2016.02056en
dc.identifier.urihttp://hdl.handle.net/10754/622690-
dc.description.abstractAutochthonous microorganisms inhabiting hydrocarbon polluted marine environments play a fundamental role in natural attenuation and constitute promising resources for bioremediation approaches. Alcanivorax spp. members are ubiquitous in contaminated surface waters and are the first to flourish on a wide range of alkanes after an oil-spill. Following oil contamination, a transient community of different Alcanivorax spp. develop, but whether they use a similar physiological, cellular and transcriptomic response to hydrocarbon substrates is unknown. In order to identify which cellular mechanisms are implicated in alkane degradation, we investigated the response of two isolates belonging to different Alcanivorax species, A. dieselolei KS 293 and A. borkumensis SK2 growing on n-dodecane (C12) or on pyruvate. Both strains were equally able to grow on C12 but they activated different strategies to exploit it as carbon and energy source. The membrane morphology and hydrophobicity of SK2 changed remarkably, from neat and hydrophilic on pyruvate to indented and hydrophobic on C12, while no changes were observed in KS 293. In addition, SK2 accumulated a massive amount of intracellular grains when growing on pyruvate, which might constitute a carbon reservoir. Furthermore, SK2 significantly decreased medium surface tension with respect to KS 293 when growing on C12, as a putative result of higher production of biosurfactants. The transcriptomic responses of the two isolates were also highly different. KS 293 changes were relatively balanced when growing on C12 with respect to pyruvate, giving almost the same amount of upregulated (28%), downregulated (37%) and equally regulated (36%) genes, while SK2 transcription was upregulated for most of the genes (81%) when growing on pyruvate when compared to C12. While both strains, having similar genomic background in genes related to hydrocarbon metabolism, retained the same capability to grow on C12, they nevertheless presented very different physiological, cellular and transcriptomic landscapes.en
dc.description.sponsorshipThis work was funded by FP-7 project Kill Spill (No. 312139,en
dc.publisherFrontiers Media SAen
dc.relation.urlhttp://journal.frontiersin.org/article/10.3389/fmicb.2016.02056/fullen
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectAlcanivoraxen
dc.subjecttranscriptomicsen
dc.subjectalkanesen
dc.subjectbioremediationen
dc.subjectfunctional redundancyen
dc.titleHydrocarbonoclastic Alcanivorax Isolates Exhibit Different Physiological and Expression Responses to n-dodecaneen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalFrontiers in Microbiologyen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionCentre for Microbial Ecology and Technology, Ghent University, Ghent, Belgiumen
dc.contributor.institutionDepartment of Food, Environmental and Nutritional Sciences, University of Milan, Milan, Italyen
dc.contributor.institutionDepartment of Medical and Forensic Pathology, University of Ghent, Ghent, Belgiumen
dc.contributor.institutionSchool of Biomedical Sciences, University of Ulster, Coleraine, UKen
kaust.authorDaffonchio, Danieleen
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