Root-associated bacteria promote grapevine growth: from the laboratory to the field

Handle URI:
http://hdl.handle.net/10754/622626
Title:
Root-associated bacteria promote grapevine growth: from the laboratory to the field
Authors:
Rolli, Eleonora; Marasco, Ramona ( 0000-0003-4776-7519 ) ; Saderi, Stefano; Corretto, Erika; Mapelli, Francesca; Cherif, Ameur; Borin, Sara; Valenti, Leonardo; Sorlini, Claudia; Daffonchio, Daniele ( 0000-0003-0947-925X )
Abstract:
Background and Aims: Laboratory and greenhouse experiments have shown that root-associated bacteria have beneficial effects on grapevine growth; however, these effects have not been tested in the field. Here, we aimed to demonstrate whether bacteria of different geographical origins derived from different crop plants can colonize grapevine to gain a beneficial outcome for the plant leading to promote growth at the field scale. Methods: To link the ecological functions of bacteria to the promotion of plant growth, we sorted fifteen bacterial strains from a larger isolate collection to study in vitro Plant Growth Promoting (PGP) traits. We analysed the ability of these strains to colonise the root tissues of grapevine and Arabidopsis using green-fluorescent-protein-labelled strain derivatives and a cultivation independent approach. We assessed the ability of two subsets randomly chosen from the 15 selected strains to promote grapevine growth in two field-scale experiments in north and central Italy over two years. Parameters of plant vigour were measured during the vegetative season in de novo grafted vine cuttings and adult productive plants inoculated with the bacterial strains. Results: Beneficial bacteria rapidly and intimately colonized the rhizoplane and the root system of grapevine. In the field, plants inoculated with bacteria isolated from grapevine roots out-performed untreated plants. In both the tested vineyards, bacteria-promotion effects largely rely in the formation of an extended epigeal system endowed of longer shoots with larger diameters and more nodes than non-inoculated plants. Conclusions: PGP bacteria isolated in the laboratory can be successfully used to promote growth of grapevines in the field. The resulting larger canopy potentially increased the photosynthetic surface of the grapevine, promoting growth.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Rolli E, Marasco R, Saderi S, Corretto E, Mapelli F, et al. (2016) Root-associated bacteria promote grapevine growth: from the laboratory to the field. Plant and Soil. Available: http://dx.doi.org/10.1007/s11104-016-3019-6.
Publisher:
Springer Nature
Journal:
Plant and Soil
Issue Date:
18-Aug-2016
DOI:
10.1007/s11104-016-3019-6
Type:
Article
ISSN:
0032-079X; 1573-5036
Sponsors:
This research was supported by the EU project BIODESERT (European Community’s Seventh Framework Programme CSA-SA REGPOT-2008-2 under grant agreement no. 245746) and King Abdullah University of Science and Technology (KAUST). ER and FM acknowledge support by Università degli Studi di Milano, DeFENS, the European Social Fund (FSE) and Regione Lombardia (contract ‘Dote Ricerca’). The authors would like to thank the “Arnaldo Caprai” and “Castello Bonomi” farms for their kind availability and interest in performing the field trials in their vineyards. The authors would like to thank Usama A. El-Behairy and Ayman F. Abou-Hadid (Ain Shams University, Cairo, Egypt) and Roberto Gerbino (Fondazione Bussolera Branca, Casteggio, Italy) for support in grapevine sampling, Umberto Fascio (CIMA, Centro Interdipartimentale di Microscopia Avanzata, University of Milan, Milan, Italy) for technical support with the confocal microscope and the “Vitis Rauscedo” grapevine nursery for kindly providing the grapevine plantlets. The authors dedicate this work to Nicola Rubaga.
Additional Links:
http://link.springer.com/article/10.1007%2Fs11104-016-3019-6
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorRolli, Eleonoraen
dc.contributor.authorMarasco, Ramonaen
dc.contributor.authorSaderi, Stefanoen
dc.contributor.authorCorretto, Erikaen
dc.contributor.authorMapelli, Francescaen
dc.contributor.authorCherif, Ameuren
dc.contributor.authorBorin, Saraen
dc.contributor.authorValenti, Leonardoen
dc.contributor.authorSorlini, Claudiaen
dc.contributor.authorDaffonchio, Danieleen
dc.date.accessioned2017-01-02T13:45:00Z-
dc.date.available2017-01-02T13:45:00Z-
dc.date.issued2016-08-18en
dc.identifier.citationRolli E, Marasco R, Saderi S, Corretto E, Mapelli F, et al. (2016) Root-associated bacteria promote grapevine growth: from the laboratory to the field. Plant and Soil. Available: http://dx.doi.org/10.1007/s11104-016-3019-6.en
dc.identifier.issn0032-079Xen
dc.identifier.issn1573-5036en
dc.identifier.doi10.1007/s11104-016-3019-6en
dc.identifier.urihttp://hdl.handle.net/10754/622626-
dc.description.abstractBackground and Aims: Laboratory and greenhouse experiments have shown that root-associated bacteria have beneficial effects on grapevine growth; however, these effects have not been tested in the field. Here, we aimed to demonstrate whether bacteria of different geographical origins derived from different crop plants can colonize grapevine to gain a beneficial outcome for the plant leading to promote growth at the field scale. Methods: To link the ecological functions of bacteria to the promotion of plant growth, we sorted fifteen bacterial strains from a larger isolate collection to study in vitro Plant Growth Promoting (PGP) traits. We analysed the ability of these strains to colonise the root tissues of grapevine and Arabidopsis using green-fluorescent-protein-labelled strain derivatives and a cultivation independent approach. We assessed the ability of two subsets randomly chosen from the 15 selected strains to promote grapevine growth in two field-scale experiments in north and central Italy over two years. Parameters of plant vigour were measured during the vegetative season in de novo grafted vine cuttings and adult productive plants inoculated with the bacterial strains. Results: Beneficial bacteria rapidly and intimately colonized the rhizoplane and the root system of grapevine. In the field, plants inoculated with bacteria isolated from grapevine roots out-performed untreated plants. In both the tested vineyards, bacteria-promotion effects largely rely in the formation of an extended epigeal system endowed of longer shoots with larger diameters and more nodes than non-inoculated plants. Conclusions: PGP bacteria isolated in the laboratory can be successfully used to promote growth of grapevines in the field. The resulting larger canopy potentially increased the photosynthetic surface of the grapevine, promoting growth.en
dc.description.sponsorshipThis research was supported by the EU project BIODESERT (European Community’s Seventh Framework Programme CSA-SA REGPOT-2008-2 under grant agreement no. 245746) and King Abdullah University of Science and Technology (KAUST). ER and FM acknowledge support by Università degli Studi di Milano, DeFENS, the European Social Fund (FSE) and Regione Lombardia (contract ‘Dote Ricerca’). The authors would like to thank the “Arnaldo Caprai” and “Castello Bonomi” farms for their kind availability and interest in performing the field trials in their vineyards. The authors would like to thank Usama A. El-Behairy and Ayman F. Abou-Hadid (Ain Shams University, Cairo, Egypt) and Roberto Gerbino (Fondazione Bussolera Branca, Casteggio, Italy) for support in grapevine sampling, Umberto Fascio (CIMA, Centro Interdipartimentale di Microscopia Avanzata, University of Milan, Milan, Italy) for technical support with the confocal microscope and the “Vitis Rauscedo” grapevine nursery for kindly providing the grapevine plantlets. The authors dedicate this work to Nicola Rubaga.en
dc.publisherSpringer Natureen
dc.relation.urlhttp://link.springer.com/article/10.1007%2Fs11104-016-3019-6en
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectBiofertilizeren
dc.subjectField experimenten
dc.subjectGrapevineen
dc.subjectPlant growth promoting bacteriaen
dc.titleRoot-associated bacteria promote grapevine growth: from the laboratory to the fielden
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalPlant and Soilen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Food, Environmental and Nutritional Sciences, DeFENS, University of Milan, via Celoria 2, Milan, 20133, Italyen
dc.contributor.institutionAustrian Institute of Technology, 2444, Seibersdorf, Austriaen
dc.contributor.institutionLaboratory BVBGR, ISBST, University of Manouba, La Manouba, 2010, Tunisiaen
dc.contributor.institutionDepartment of Agricultural and Environmental Sciences, Production, Landscape, Agroenergy, DISAA, University of Milan, via Celoria 2, Milan, 20133, Italyen
kaust.authorMarasco, Ramonaen
kaust.authorDaffonchio, Danieleen
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